RESUMO
BACKGROUND: It is important to identify the behavior of colorectal cancer (CRC) individually, so more accurate laboratory index is urgently demanded. Chaperonin are key molecules in tumor cell cycle. Our study aims at revealing the expression and correlation of chaperonin containing TCP1 complex 6A (CCTζ-1) in CRC. METHODS: Fifty-eight patients with CRC admitted from 2005 to 2008 were selected. CCTζ-1 expression in cell, tumor, and non-tumor colorectal tissues was detected by Western blot, and their protein was localized by immunohistochemical stain. After HCT116 cells were transfected with CCTζ-1 siRNA, real-time PCR, and Western blot were used to examine gene expression. Cell multiplication and apoptosis were examined by Cell Counting Kit-8 and Annexin V kit. RESULTS: CCTζ-1 ptotein expression was detected in 51 of 58 (87.9%) CRC specimens, which was much higher than those in normal mucosa (P < 0.01), and it was correlated with tumor invasion (P < 0.01) and tumor size (P < 0.05). The levels of CCTζ-1 mRNA and protein were inhibited by CCTζ-1 siRNA in HCT116 cells transfected with CCTζ-1 siRNA, which resulted in growth arrest but not apoptosis. CONCLUSION: CCTζ-1 could be a new prognostic marker for CRC and involve in tumorigenesis. CCTζ-1 inhibition in vivo might therefore become a new therapy for CRC.
Assuntos
Proliferação de Células , Chaperonina com TCP-1/metabolismo , Neoplasias Colorretais/metabolismo , Regulação Neoplásica da Expressão Gênica , Proteínas de Neoplasias/metabolismo , Idoso , Chaperonina com TCP-1/antagonistas & inibidores , Chaperonina com TCP-1/genética , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Feminino , Células HCT116 , Humanos , Técnicas In Vitro , Masculino , Pessoa de Meia-Idade , Proteínas de Neoplasias/antagonistas & inibidores , Proteínas de Neoplasias/genética , RNA Mensageiro/metabolismo , RNA Interferente PequenoRESUMO
Prof. XIE Xi-liang in his early years was a disciple of Mr. CHENG Dan-an. He has been engaged in clinical work and teaching for over 60 years with rich experience, and he pays attention to application of acupuncture, moxibustion and medicine for different persons, especially pays attention to moxibustion methods, such as direct moxibustion, with less points selected, and good results, convenient manipulation, moxibustion of one session only for 3-5 min. He teaches the patients and their family for self-moxibustion at home, saving time. This method has better short-term and long-term therapeutic effects for hypo-immune patients, pain diseases, and it is suitable to treatment of diseases and health-care.
