Synergistic Cytotoxicity of Chemotherapeutic Agents and Tumor Necrosis Factor ? against Hepatic Carcinoma Cells in vitro / 中国肿瘤生物治疗杂志

The synergistic cytotoxicity of rhTNF-? or/and six chemotherapeutic agents namely ADM, MMC, DDP, VP-16, GBP and IFO, against two human hepatic carcinoma cell lines, SMMC-7721 and BEL-7402, was examined by MTT assay. The results showed that the cytotoxic effects of six chemotherapeutic agents with rhTNF-? against two cell lines were significant. Examined cytotoxicity of rhTNF-? combined with chemotherapeutic agents, we found that rhTNF-? singnificantly enhanced cytotoxic efficacy of ADM, DDP, VP-16. The study suggested that rhTNF-? with some chemotherapeutic agents had synergistic effects. This and other preclinical studies with rhTNF-? will form the basis of clinical treatments for hepatic carcinoma patient.