Infant limbus: an immunohistological study.

All published literature to date has identified the human corneo-scleral limbus as the site within which stem cells of the ocular surface reside. Recently we described a unique anatomical structure at the limbus, termed the Limbal Epithelial Crypt (LEC) that has features of a putative stem cell niche. In this study we examined infant limbus tissue (donor age 4 months) for evidence of LEC and performed immunohistological comparison between infant limbus and adult LEC. No defined LEC were detected in the infant limbus. However, the entire infant limbus has characteristics resembling adult LEC. Both infant limbus and LEC demonstrated negative expression for desmoglein 3. p63 and integrin beta1 expressions were located to the distal region of the infant limbus and to the basal region of the LEC. ABCG2 expression was positive throughout most of the infant limbus as was connexin 43. Infant limbus and in particular the distal region, appeared to house cells that are more "stem-like" in nature. The LEC may be a result of normal physiological developmental in order to protect and maintain stem cells at the ocular surface.

Patients' attitudes towards trainee surgeons performing cataract surgery at a teaching hospital.

AIM: To evaluate patients' preferences of surgeon to perform their cataract surgery if given a choice between consultant and trainee. METHODS: A questionnaire based patient satisfaction survey was conducted in a large University Teaching Hospital in the UK. One hundred and eighty patients undergoing first eye cataract surgery between January and March 2006 were asked a number of set questions on their preferences regarding the surgeon performing the operation. Primary outcome measure was the patient's preference for who would perform their cataract surgery (consultant or trainee). RESULTS: Overall, 126 (70%) accepted that trainee surgeons should operate as part of their training. Only 102 (81%) of these (57% of the total) would be happy to be operated on themselves by a supervised surgical trainee. Ninety-eight (78%) patients objected to being operated on by a trainee if they were to be unsupervised. One hundred and forty-two (79%) patients stated they would choose to wait longer for their surgery if it meant that a consultant would perform their operation. This preference was held significantly more strongly among patients who had been listed for surgery from a consultant's clinic rather than from the pooled 'cataract clinic' (P=0.048). One hundred and forty-four (80%) patients thought they should be told the name and designation of the surgeon who was to perform their operation. CONCLUSIONS: Patients undergoing their first cataract procedure appear to have a preference for their named consultant to perform their surgery. If 'patient choice' extends to the choice of operating surgeon, then there are clear implications for the training of future UK ophthalmologists.

Impression cytology of the ocular surface.

Impression cytology refers to the application of a cellulose acetate filter to the ocular surface to remove the superficial layers of the ocular surface epithelium. These cells can then be subjected to histological, immunohistological, or molecular analysis. Proper technique is essential as the number of cells sampled can vary considerably. Generally two to three layers of cells are removed in one application but deeper cells can be accessed by repeat application over the same site. Applications for impression cytology include diagnosing a wide range of ocular surface disorders, documenting sequential changes in the conjunctival and corneal surface over time, staging conjunctival squamous metaplasia, and monitoring effects of treatment. It is also a useful investigational tool for analysing ocular surface disease with immunostaining and DNA analysis. It is non-invasive, relatively easy to perform, and yields reliable information about the area sampled with minimal discomfort to the patient. Major ophthalmic centres should develop and introduce this technique into routine clinical practice. This is best achieved with a team approach including the ophthalmologist, pathologist, microbiologist, and the immunologist.