RESUMO
A UV-generated mutant of Fusarium sporotrichioides NRRL 3299 was altered in its ability to biosynthesize T-2 toxin, as shown by a rapid screen with monoclonal antibodies to T-2. This stable mutant accumulated two trichothecenes that were not observed in liquid cultures of the parent strain. The two compounds were identified as 3,15-diol 12,13-epoxytrichothec-9-ene and 3,15-diol 12,13-epoxytrichothec-9-ene 3-acetate on the basis of their nuclear magnetic resonance and mass spectra. This is the first report of either of these two compounds as secondary metabolites of F. sporotrichioides and of a trichothecene acetylated at C-3 by this species.
Assuntos
Fusarium/crescimento & desenvolvimento , Mutação , Sesquiterpenos/metabolismo , Tricotecenos/metabolismo , Cromatografia Líquida de Alta Pressão , Meios de Cultura , Fusarium/genética , Fusarium/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Tricotecenos/isolamento & purificaçãoAssuntos
Ácidos Anacárdicos/química , Ácidos Anacárdicos/isolamento & purificação , Myristicaceae/química , Plantas Medicinais/química , Alcanos/química , Alcanos/isolamento & purificação , Derivados de Benzeno/química , Derivados de Benzeno/isolamento & purificação , Lignanas/química , Lignanas/isolamento & purificação , Estrutura Molecular , Óleos/química , Sementes/químicaRESUMO
Curvularin, a fungal metabolite similar in chemical structure to zearalenone, a potent estrogen, was tested for its estrogenic effects to gilts. No estrogenicity was observed to 60 kg gilts after feeding curvularin per os at a rate of 10 mg per day for 5 days. Curvularin was also nontoxic to mice and chick embryo.
Assuntos
Estrogênios não Esteroides , Penicillium/metabolismo , Resorcinóis , Zearalenona , Animais , Embrião de Mamíferos/efeitos dos fármacos , Estrogênios não Esteroides/análise , Estrogênios não Esteroides/biossíntese , Estrogênios não Esteroides/toxicidade , Feminino , Penicillium/classificação , Resorcinóis/análogos & derivados , Resorcinóis/análise , Resorcinóis/biossíntese , Resorcinóis/toxicidade , Suínos , Zearalenona/análogos & derivados , Zearalenona/análise , Zearalenona/biossíntese , Zearalenona/toxicidadeRESUMO
Interesting differences were found in oils from two samples ofStenachaenium macrocephalum (Compositae) seed with dissimilar storage histories. One contained significant amounts of epoxy acids (6.5%) and hydroxy conjugated dienoic acids (5.6%), but the other contained no more than 1% of these oxygenated acids. Characterization of components in the former oil established that the principal epoxy acid (4.0%) is the previously unknowncis-9, 10-epoxy-trans-3,cis-12-octadecadienoic acid. The conjugated dienols include two additional new acids with Δ3 unsaturation (2.5%): 9-hydroxy-trans-3,-trans-10,cis-12-octadecatrienoic and 13-hydroxy-trans-3,cis-9,trans-11-octadecatrienoic acoids. The nonoxygenated acids, except for the large amount (40%) oftrans-3,cis-9,cis-12-octadecatrienoic, are those that commonly occur in seed oils.
