Epidemiological Aspects of Escherichia albertii Outbreaks in Japan and Genetic Characteristics of the Causative Pathogen.

Zoonotic pathogen Escherichia albertii has been identified as the cause of several human disease outbreaks; however, factors such as the general symptoms and incubation period of E. albertii infection have yet to be defined. Therefore, we aimed to determine the unique aspects of E. albertii outbreaks in Japan and to examine the genetic characteristics of the causative pathogen. We studied all known E. albertii outbreaks that occurred in Japan up until 2015, which consisted of five confirmed outbreaks and one putative outbreak (Outbreaks 1-6). Outbreaks were re-examined based on personal communications between researchers in prefectural and municipal public health institutes, and through examination of any published study conducted at the time. Draft genome sequences of outbreak-associated E. albertii isolates were also generated. The most common symptom displayed by patients across the six episodes was watery diarrhea (>80%), followed by abdominal pain (50-84%) and fever (37.0-39.5°C) (26-44%). The estimated average incubation period of E. albertii infection was 12-24 h. We assumed that most of the outbreaks were foodborne or waterborne, with restaurant foods, restaurant water, and boxed lunches being the suspected transmission vehicles. Three of the six outbreak-associated E. albertii isolates possessed intact ETT2 regions, while the remaining isolates contained disrupted ETT2-encoding genes. Virulence gene screening revealed that more than half (44/70) of the tested genes were present in all 5 strains examined, and that each of the strains contained more than 1 gene from 14 out of the 21 groups of virulence genes examined in this study. The five E. albertii strains were classified into four of the five known phylogroups. Therefore, we determined that multiple E. albertii genotypes in Japan have the potential to cause outbreaks of diarrhea, abdominal pain, and/or fever following infection of a human host.

The effect of pregnancy on the progression of diabetic retinopathy.

PURPOSE: To evaluate the progression and risk factors of diabetic retinopathy during and after pregnancy in patients with diabetes. METHODS: We checked the medical records of the patients with diabetes who were referred to the Diabetes Center, Tokyo Women's Medical University Hospital (Tokyo, Japan), and were pregnant between August 2004 and September 2010. The patients whose ophthalmic examinations records could not be found (n = 15) were excluded, and thus 93 patients (type 1 = 68, type 2 = 25) were included in the analyses. Data were obtained from the medical record and evaluated. RESULTS: The mean pre-pregnancy HbA1c was 7.1 ± 0.8 %. All patients were encouraged to maintain HbA1c level of under 7.4 % before pregnancy. At baseline, the percentage of patients with no retinopathy was 78 %, with nonproliferative retinopathy 14 %, and with proliferative retinopathy, 8 %. Progression of retinopathy occurred in 16 (17 %) patients. Two (2 %) had loss of visual acuity and 1 (1 %) underwent laser treatment due to progression of retinopathy. Compared with patients who did not show progression, the patients with progression were characterized by longer duration of diabetes (p < 0.00001), presence of diabetic retinopathy before pregnancy (p < 0.00001) and higher blood pressure in the second trimester (p < 0.05). CONCLUSIONS: Frequent ophthalmic examination, especially in patients with a long history of diabetes, is recommended, as well as for patients with presence of diabetic retinopathy before pregnancy. In contrast to previous reports, the progression of retinopathy in this study was lower. This is probably due to our patients' pre-pregnancy tight glucose control and the high ratio of patients with no retinopathy at baseline.

Learning curve for paramedic endotracheal intubation and complications.

BACKGROUND: Pre-hospital laryngoscopic endotracheal intubation (ETI) is potentially a life-saving procedure but is a technique difficult to acquire. This study aimed to obtain a recommendation for the number of times ETI should be practiced by constructing the learning curve for endotracheal intubation by paramedics, as well as to report the change in the frequency of complications possibly associated with intubation over the training period. METHODS: Under training conditions, 32 paramedics performed a total of 1,045 ETIs in an operating room. Trainees performed ETIs until they succeeded in 30 cases. For each patient, the number of laryngoscopic maneuvers and any complications potentially associated with ETI were recorded. We built a generalized logistic model to construct the learning curve for ETI and the frequency of complications. RESULTS: During the training on the first 30 patients the rate of ETI success at the first attempt improved from 71% to 87%, but there was little improvement during the first 13 cases. The frequency of complications decreased from 53% to 31%. More laryngoscopic maneuvers and longer operation time increased complications. CONCLUSIONS: It seems that 30 live experiences of performing an ETI is sufficient for obtaining a 90% ETI success rate, but there seems to be little benefit with fewer than 13 experiences. The frequency of complications remained at a high level even after the training. It is desirable to conduct a more detailed and rigorous evaluation of the benefit of pre-hospital ETI by controlling for the skill level of paramedics.

Systemic complications of posterior subtenon injection of triamcinolone acetonide in type 2 diabetes patients.

We evaluated the influence of posterior subtenon injection of triamcinolone acetonide on blood glucose and blood pressure in type 2 diabetes patients. This treatment, in this limited series, did not significantly increase HbA1c levels or blood pressures. But spike-like increases of fasting blood glucose were observed in some cases.

Injection of triamcinolone acetonide into the posterior sub-tenon capsule for treatment of diabetic macular edema.

PURPOSE: To compare eyes that received treatment with untreated fellow eyes to assess the effectiveness of injection of 20 mg of triamcinolone acetonide (TA) into the posterior sub-Tenon capsule for treatment of diabetic macular edema (DME). METHODS: Nine adult patients (mean age, 60.4 years) with DME in both eyes were followed up for 3 months after unilateral treatment. We compared central macular thickness before and after treatment with those of the untreated fellow eye. RESULTS: In 9 eyes injected with 20 mg of TA, the mean central macular thickness +/- SD was 624.8 +/- 173.7 microm before treatment. In the fellow eyes, it was 452.8 +/- 235.2 microm. There was no significant difference between the two groups (P=0.10, unpaired t-test). One month after injection, it was 434.0 +/- 120.7 microm in injected eyes (P=0.017, paired t-test) and 462.2 +/- 232.7 microm in fellow eyes (P=0.70, paired t-test), and after 3 months, the respective values were 423.1 +/- 186.3 microm (P=0.052, paired t-test) and 478.3 +/- 269.1 microm (P=0.65, paired t-test). CONCLUSION: Injection of 20 mg of TA into the posterior sub-Tenon capsule effectively reduces macular thickening due to DME, at least in the short term.

Posterior capsule opacification after combined cataract surgery and vitrectomy.

PURPOSE: To compare the degree of posterior capsule opacification (PCO) after combined cataract surgery and vitrectomy and after cataract surgery alone in age-matched control patients and in patients with and without diabetes mellitus (DM). SETTING: Sugita Eye Hospital, Nagoya, Japan. METHOD: In a prospective study, patients were divided into 2 groups: those having cataract surgery alone (cataract group) and those having combined cataract and vitrectomy surgery (combined group). The 2 groups were further divided into patients without DM (non-DM subgroup) and patients with DM (DM subgroup). The PCO rates were determined 3, 6, and 12 months after surgery using the POCO system. RESULTS: In the cataract group, the non-DM subgroup and the DM subgroup each comprised 42 eyes of 42 patients. In the combined group, the non-DM subgroup comprised 26 eyes of 26 patients and the DM subgroup, 23 eyes of 23 patients. In the cataract group, the PCO rate in the DM subgroup was significantly higher than in the non-DM subgroup at every postoperative examination (P<.05). In the non-DM subgroup, the PCO rate was significantly higher at every postoperative examination in patients having combined surgery than in patients having cataract surgery alone (P<.05). In the combined group, the PCO rate did not differ significantly between the non-DM subgroup and the DM subgroup. CONCLUSION: Even patients without DM must be carefully monitored for PCO development after combined surgery.