RESUMO
BACKGROUND: Wound contractures can cause severe deformities and disabilities. Recent studies have suggested that leukotriene receptor antagonists have an inhibitory effect on the healing contraction process. This study aimed to evaluate the influence of the leukotriene inhibitor montelukast on the cutaneous healing process and the wound contraction phenomenon in rats. METHODS: For this study, 60 male rats were randomly divided into four groups (MK-7d, SF-7d, MK-14d, and SF-14d) according to the drug given through a rigid orogastric tube (MK group: montelukast 10 mg/kg/day; SF group: normal saline solution) and the day the animals were killed (7d: postoperative day 7; 14d: postoperative day 14). An excisional wound (2 x 2 cm) was created on the dorsum of each rat. The wounds were left open to heal spontaneously and documented by standard digital photographs on different postoperative days. Wound contraction rates were calculated with specific software, and specimens were histologically evaluated using picrosirius red stain. Results were analyzed using the Aspin-Welch, Mann-Whitney, and t tests, assuming a significance level of 5%. RESULTS: The wound contraction rates were similar between the control and study groups (p > 0.05). On postoperative day 7, the wounds showed a marginally significant reduction in collagen maturation in the study group (40.1% +/- 6.88% vs 61.2% +/- 8.02%; p = 0.0607). On postoperative day 14, this reduction was statistically significant in the MK group (26% +/- 5.66% vs 68.3% +/- 7.76%; p = 0.0001). CONCLUSIONS: Montelukast does not alter the contraction rate of excisional wounds in rats but has a significant and progressive inhibitory effect on collagen maturation.
Assuntos
Acetatos/administração & dosagem , Colágeno/efeitos dos fármacos , Procedimentos Cirúrgicos Dermatológicos , Antagonistas de Leucotrienos/administração & dosagem , Quinolinas/administração & dosagem , Cicatrização/efeitos dos fármacos , Animais , Colágeno/metabolismo , Ciclopropanos , Modelos Animais de Doenças , Masculino , Probabilidade , Distribuição Aleatória , Ratos , Ratos Wistar , Valores de Referência , Sensibilidade e Especificidade , Pele/lesões , Estatísticas não Paramétricas , SulfetosRESUMO
INTRODUCTION: Wound healing is a complex process that deals with different biological and immunological systems and is essential to keep the organism integrity. Three well-defined phases occur: inflammatory, proliferative and maturation. A failure or lengthy phase may result in a delay or absence of it. PURPOSE: The aim of this paper is to analyse comparatively the histological alteration provided by the use of the aqueous extract of Orbignya phalerata, in the healing process of bladder surgical wounds. METHODS: Forty adult, male Wistar rats were used. The experimental procedure consisted of a longitudinal 2 cm long bladder incision and single layer interrupted suture of 5-0 polyglactine 910. Post-operatively, the rats were randomly divided into two groups of 20. The substance was not used in the control group. The Orbignya phalerata aqueous solution was used in the study group. The animals were observed and killed three and seven days later. Comparative histological analysis was accomplished between the groups. RESULTS: Significant statistical differences were observed in the neo-formation variables (p= 0.001), chronic inflammation (p= 0.002) and fibroblastic proliferation (p= 0.023). Acute inflammation was very evident in the control group. CONCLUSION: The time factor of wound healing showed homogeneity between experimental and control groups, however faster in the experimental one. The Orbignya phalerata had a favoring healing effect in the surgical incision on rats bladder.
Assuntos
Arecaceae/química , Fitoterapia , Extratos Vegetais/uso terapêutico , Bexiga Urinária/cirurgia , Cicatrização/efeitos dos fármacos , Animais , Anti-Inflamatórios/uso terapêutico , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Inflamação/tratamento farmacológico , Masculino , Ratos , Ratos Wistar , Técnicas de Sutura , Bexiga Urinária/lesões , Bexiga Urinária/patologiaRESUMO
INTRODUÇÃO: A cicatrização constitui processo complexo, envolvendo diferentes sistemas biológicos e imunológicos, sendo essencial para manter a integridade do organismo. Três fases bem definidas ocorrem: inflamatória, proliferativa e maturação. Falha ou prolongamento em uma delas pode resultar em retardo ou ausência da cicatrização. OBJETIVO: Analisar comparativamente as alterações histológicas proporcionadas pelo uso do extrato aquoso da Orbignya phalerata na cicatrização de lesões cirúrgicas da bexiga. MÉTODOS: Quarenta ratos da linhagem Wistar, adultos e machos foram utilizados. O procedimento experimental constituiu-se em incisão longitudinal de 2cm na bexiga e síntese em plano único com pontos separados de poliglactina 910 cinco zeros. Após este procedimento comum, os animais foram distribuídos aleatoriamente em dois subgrupos contendo 20 animais cada. No grupo controle não foi utilizada a substância Orbignya phalerata. No grupo-experimento utilizou-se solução aquosa de Orbignya phalerata na dose de 50mg/kg por via intraperitoneal. Os animais foram acompanhados e mortos em três e sete dias. Foi feita análise histológica comparativa entre os grupos. RESULTADOS: Os resultados mostraram que foi observada diferença estatística significante nas variáveis neoformação (p= 0,001) e proliferação fibroblástica (p= 0,010) nos subgrupos controle e experimento do 3º dia. Ainda no grupo experimento a neoformação capilar foi mais intensa que no grupo controle, apresentando significância estatística. No grupo de sete dias observou-se que as variáveis inflamação aguda (p= 0,001), inflamação crônica (p= 0,002) e proliferação fibroblástica (p=0,023) apresentaram significância estatística, sendo bem evidente a inflamação aguda no grupo controle. CONCLUSÃO: Foi observada homogeneidade no fator tempo da cicatrização nos grupos experimento e controle sendo mais rápida no grupo experimento. Houve efeito favorecedor cicatrizante na utilização do extrato aquoso do mesocarpo da Orbignya phalerata na bexiga do ratos.
INTRODUCTION: Wound healing is a complex process that deals with different biological and immunological systems and is essential to keep the organism integrity. Three well-defined phases occur: inflammatory, proliferative and maturation. A failure or lengthy phase may result in a delay or absence of it. PURPOSE: The aim of this paper is to analyse comparatively the histological alteration provided by the use of the aqueous extract of Orbignya phalerata, in the healing process of bladder surgical wounds. METHODS: Forty adult, male Wistar rats were used. The experimental procedure consisted of a longitudinal 2cm long bladder incision and single layer interrupted suture of 5-0 poliglactine 910. Post-operatively, the rats were randomly divided into two groups of 20. The substance was not used in the control group. The Orbignya phalerata aqueous solution was used in the study group. The animals were observed and killed three and seven days later. Comparative histological analysis was accomplished between the groups. Resuts: Significant statistical differences were observed in the neo-formation variables (p= 0,001), chronic inflammation (p= 0,002) and fibroblastic proliferation (p= 0,023). Acute inflammation was very evident in the control group. CONCLUSION: The time factor of wound healing showed homogeneity between experimental and control groups, however faster in the experimental one. The Orbignya phalerata had a favoring healing effect in the surgical incision on rats bladder.
