RESUMO
We have constructed a public gene expression data repository and online data access and analysis, WWW and FTP sites for serial analysis of gene expression (SAGE) data. The WWW and FTP components of this resource, SAGEmap, are located at http://www.ncbi.nlm.nih. gov/sage and ftp://ncbi.nlm.nih.gov/pub/sage, respectively. We herein describe SAGE data submission procedures, the construction and characteristics of SAGE tags to gene assignments, the derivation and use of a novel statistical test designed specifically for differential-type analyses of SAGE data, and the organization and use of this resource.
Assuntos
Bases de Dados Factuais , Expressão Gênica/genética , Internet , Feminino , Biblioteca Gênica , Humanos , Masculino , Análise de Sequência de DNA/métodos , Sitios de Sequências Rotuladas , Processamento de Sinais Assistido por ComputadorAssuntos
Perfilação da Expressão Gênica/métodos , Neoplasias da Próstata/genética , Bases de Dados Factuais , Expressão Gênica , Perfilação da Expressão Gênica/estatística & dados numéricos , Biblioteca Gênica , Humanos , Masculino , Análise de Sequência com Séries de Oligonucleotídeos , Patologia Clínica/métodos , Polimorfismo de Nucleotídeo ÚnicoAssuntos
Sequência de Bases , DNA/genética , Bases de Dados Factuais , Animais , DNA/química , Expressão Gênica , HumanosRESUMO
A computer program, OLIGO, is described which was designed to manage projects involving the synthesis of large numbers of oligonucleotide fragments using cellulose discs as immobilizing support. The program performs the functions of data entry, verification, analysis and modification; the sorting of filters before each step and project status updating after each reaction step; output of the project status at any point and storage of the finalized project data in a permanent reference file.
Assuntos
Computadores , Oligonucleotídeos/síntese química , Software , Sequência de Bases , Celulose , FiltraçãoRESUMO
A set of computer programs is described which constitutes a clone database management system. Maintenance of the database and the stocks of material is designed to be under the control of one person or group of people, who may insert, delete or modify data entries, and who may interrogate the database as to which stocks are in need of checking. The system is organised in such a way that information is freely and speedily available to all users. Database entries may be accessed by name or key word.
Assuntos
Clonagem Molecular , DNA Recombinante , Animais , Bacteriófagos/genética , Galinhas , Computadores , Enzimas de Restrição do DNA , Coleta de Dados , Eritrócitos , Escherichia coli/genética , Genes , PlasmídeosRESUMO
A computer program (RSITE) was developed which predicts the recognition sequence of a restriction endonuclease. The sizes of fragments experimentally determined on cleavage of a DNA of known sequence were input. Possible recognition sequences producing fragments of sizes matching those determined empirically were printed out. The program faithfully predicted the specificity of restriction enzymes of known recognition sequence and also determined the recognition sequence of a new restriction enzyme from Haemophilus influenzae GU (HinGU II).
