[Mechanism of the negative action of a bacterial lipopolysaccharide (pyrogenal) on the course of experimental ornithosis infection]. / O mekhanizme otritsatel'nogo deistviia bakterial'nogo lipopolisakharida (pirogenala) na techenie éksperimental'noi ornitoznoi infektsii.

The intravenous injection of pyrogenal into mice following their intravenous infection aggravates the course of ornithosis infection and leads to more intensive multiplication of the infective agent. After the injection of pyrogenal is discontinued a change in the course of the infectious process occurs and the infective agent is quickly eliminated from the body. This is accompanied by increased formation of specific antibodies. The negative effect of pyrogenal is due to its high toxicity for macrophages containing the colonies of the infective agent, the suppression of the reaction of neutrophil leukocytes at the sites of the destruction of infected cells and the suppression of the general reaction of the mononuclear phagocytic system. Pyrogenal does not damage lymphoid tissue and even produces an immunostimulant effect on it, but the increased immune response can be completely realized only after pyrogenal injections are stopped and the reaction of mononuclear phagocytes is restored.

[Effect of leukocyte cation proteins in vitro on the activity of the pathogen of meningopneumonitis]. / O vliianii in vitro kationovykh belkov leikotsitov na aktivnost' vozbuditelia meningopnevmonii.

A study was made of the influence of cation proteins from the lysosomes and nuclei of rabbit polymorphonuclear leukocytes on the survival of the causative agent of meningopneumonia in vitro. As revealed, the greatest antibiotic activity was possessed by the nuclear histones and the low molecular basic proteins of lysosomes. Biological significance of the detected effects is discussed.

[Role of leukocyte disintegration products in increasing macrophage resistance to the causative agent of orinthosis]. / O roli produktov raspada leikotsitov v povyshenii rezistentnosti makrofagov k vozubditeliu ornitoza

The dymanics of formation of macrophagal granulomas in the liver of albino mice was studied after intravenous infection with the causative agent of ornithosis. In formation of granulomas macrophages underwent the stage of reutilization of the leukocyte disintergration and became resistant to the causative agent of ornithosis. Together with the leukocyte remants histones and cation proteins could penetrate into the macrophages cytoplasm; these displayed a high antironithosis activity in vitro. An increase of macrophage resistance to the causative agent after reutilization of the products of leukocyte disintegration apparently sreved as one of the mechanisms of resistance formation in the foci of inflammation in ornithosis.

[Morphological manifestations of secretory activity of the neutrophils in inflammation]. / Morfologicheskie proiavleniia sekretornoi aktivnosti neitrofil'nykh leikotsitov pri vospalenii

Neutropholic leukocytes of aseptic peritoneal exudate of rabbits differed from neutrophilic leukocytes of the blood in greater variability in the content of cationic proteins. Some lysosomes ceased to be stained on cationic proteins, which produced a false impression of degranulation of leukocytes. Ultramicroscopically, there was observed a "clearing" of the lysosomes content and its outflow into cytoplasm. Lysosomes acquired the shape of spherical "holed" bodies and were transformed into clear vacuoles with remnants of the electron-dense material. The rupture of lysosome membranes led to an "explosive" type of liberation of the lysosome content into the cytoplasm. Partly degranulated leukocytes remained vital and activity moved about in a homologous serum. Following contacts with histone F1 leukocytes lost cationic proteins. The liberation of cationic proteins from leukocytic took place without destruction of leukocytes and lysosomes themselves.

[Effect of dilysosomal macrophages on the development and outcome of pneumonia induced by the causative agent of enzootic abortion of sheep]. / Vliianie dilizosomnykh makrofagov na razvitie i iskhod pnevmonii, vyzvannoi vozbuditelem énzooticheskogo aborta ovets

Repeated intranasal injections of increasing doses of bacterial lipopolysaccharide pyrogenal resulted in accumulation of numerous macrophages in the upper and middle parts of the mouse lungs; these macrophages were filled with leukocyte lysosomes. In these animals dilysosomal macrophages increased local resistance to the agent of enzootic ewe abortion and thus could change the lethal form of induced pneumonia into the nonlethal one.

[In vitro effect of leukocytic and thymic histones and their fractions on the activity of the causative agent of meningopneumonia]. / O vliianii in vitro leikotsitarnogo i timusnogo gistonov i ikh fraktsii na aktivnost' vozbuditelia meningopnevmonii

It was found in studying the antimeningococcus activity of the leukocytic and thymus histones and their fractions that both histones were capable of neutralizing in vitro the activity of the causative agent of meningopneumonia (MP). The neutralization effect was chiefly associated with the F3 fraction rich in arginine and depended on the duration of the histone fraction contact with the MP causative agent, the weight concentration of the histone and the pH of the incubation medium.

[Morphological manifestations of the intracellular toxic effect of microorganisms of the ornithosis-lymphogranuloma-trachoma group]. / Morfologicheskie proiavleniia vnutrikletochnogo toksicheskogo deistviia u vozbuditelei iz gruppy ornitoza-limfogranulemy-trakhomy

Interactions of the causative agents of meningopneumonia and ovine abortion with pulmonary macrophages in experimental infection in white mice were studied by histological, immunofluorescent and electron microscopic methods. The causative agent of meningopneumonia did not multiply in colonies and caused toxic lesions in the cells and formed cytoplasmic oxiphilic inclusion bodies. Being the product of cell degeneration, these oxiphilic inclusion bodies were conducive to long-term survival of the cell and slow release from them of elementary bodies of meningopneumonia agent. The agent of ovine abortion multiplied in colonies without toxic affection of the cells or formation of oxiphilic inclusion bodies. It is suggested that oxiphilic inclusion bodies represent morphological manifestation of the intracellular toxic effect of rickettsial and ornithosis-like agents possessing dissociated (non-colonial) mode of multiplication.