Streptococcus mitis and Prevotella melaninogenica Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients.

Oral microbiome disruptions in periodontitis are related to the chronic inflammatory reactions that could in turn lead to the development of multiple oral diseases. The objective of the study was to assess the frequencies of Streptococcus mitis, Prevotella melaninogenica, and Prevotella intermedia in oral benign lesions, oral potentially malignant disorders (OPMDs), and oral squamous cell carcinomas (OSCCs) and investigate the impact of these bacteria on the expression patterns of the selected (potential) target genes (PI3CA/AKT2/mTOR, DUSP16/MAPK14, and COX2). After sample collection (25 benign lesions, 30 OPMDs, and 35 OSCCs) and DNA/RNA extraction, quantitative real-time polymerase chain reaction (qPCR) was performed to detect bacterial presence and assess relative gene expression levels in different lesion groups. Prevotella melaninogenica was the most prevalent of the three analyzed bacteria, with the frequency being 60% in benign lesions, 87% in OPMDs (p = 0.024), and 77% in OSCC. The OPMD tissues in which Prevotella melaninogenica was present exhibited a higher expression level of AKT2 (p = 0.042). Significantly lower expression of DUSP16 was observed in OSCC tissues containing Streptococcus mitis (p = 0.011). The obtained results indicate a substantial contribution of P. melaninogenica and Str. mitis in the pathogenesis of oral mucosal lesions, possibly via AKT2 upregulation and DUSP16 downregulation.

The MOBIS dataset: a large GPS dataset of mobility behaviour in Switzerland.

This article presents the MOBIS dataset and underlying survey methods used in its collection. The MOBIS study was a nation-wide randomised controlled trial (RCT) of transport pricing in Switzerland, utilising a combination of postal recruitment, online surveys, and GPS tracking. 21,571 persons completed the first online survey, and 3680 persons completed 8 weeks of GPS tracking. Many continued tracking for over a year after the study was completed. In the field experiment, participants participated through the use of a GPS tracking app, Catch-my-Day, which logged their daily travel on different transport modes and imputed the trip segments and modes. The experiment lasted 8 weeks, bookended by two online surveys. After the first 4-week control phase, participants were split into two different treatment groups and a continued control group. An analysis of the survey participation shows that the technology is capable of supporting such an experiment on both Android and iOS, the two main mobile platforms. Significant differences in the engagement and attrition were observed between iOS and Android participants over the 8-week period. Finally, the attrition rate did not vary between treatment groups. This paper also reports on the wealth of data that are being made available for further research, which includes over 3 million trip stages and activities, labelled with transport mode and purpose respectively. Supplementary Information: The online version contains supplementary material available at 10.1007/s11116-022-10299-4.

Exploring the role of positive direct experience in the adoption of energy efficient technologies: evidence from a Swiss field study on the promotion of low-flow showerheads.

Despite the considerable potential of low-flow showerheads to reduce household energy demand, their widespread implementation is still far from being realised. In this study, we compare the joint effect of a contextually embedded intervention in a public swimming pool to promote low-flow showerheads coupled with a mass campaign by a Swiss city's utility to the stand-alone effect of the mass campaign. We also explore the factors that influence the outcome of the contextually embedded intervention. The quasi-experimental design of the study was possible due to the co-occurring installation of low-flow showerheads in a local public swimming pool and a campaign of a local utility, which offered low-flow showerheads for domestic use at a substantially reduced price. Our findings showed that the combined intervention was substantially more effective than the mass campaign alone. However, this result has to be interpreted with caution owing to the imperfect comparability of the two campaigns. Based on a survey of 402 swimming pool visitors, the study findings demonstrate the crucial role of a positive direct experience in the promotion of low-flow showerheads. This had a significant positive impact on attitudes towards low-flow showerheads, which in turn was found to be the most important determinant of purchase intention. The results suggest that more active communication of energy efficiency measures in public facilities might contribute to reductions in household energy use. Such campaigns can be used to share experiences of energy efficiency technologies and, therefore, promote the use of similar systems at home.