Assuntos
Infecções por Pseudomonas , Pseudomonas aeruginosa , beta-Lactamases , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/enzimologia , Pseudomonas aeruginosa/isolamento & purificação , Pseudomonas aeruginosa/efeitos dos fármacos , Brasil , Humanos , beta-Lactamases/genética , Infecções por Pseudomonas/microbiologia , Infecções por Pseudomonas/epidemiologia , Genoma Bacteriano , Antibacterianos/farmacologia , Testes de Sensibilidade Microbiana , GenômicaRESUMO
We aimed to analyze the molecular epidemiology of 46 methicillin-resistant Staphylococcus aureus (MRSA) isolated from breast infections. The USA300 lineage carrying SCCmecIVa, arginine catabolic mobile element, t008, ST8, and Panton-Valentine leukocidin genes was predominant (93%). This is the first study that describes the spread of the USA300 methicillin-resistant Staphylococcus aureus clone in breast infections in Brazil.
Assuntos
Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Humanos , Staphylococcus aureus Resistente à Meticilina/genética , Brasil/epidemiologia , Infecções Estafilocócicas/epidemiologia , Exotoxinas/genética , Leucocidinas/genética , Epidemiologia Molecular , DNA Bacteriano/genéticaRESUMO
Noroviruses are the leading cause of acute gastroenteritis (AGE) in all age groups worldwide. Despite the high genetic diversity of noroviruses, most AGE outbreaks are caused by a single norovirus genotype: GII.4. Since 1995, several different variants of norovirus GII.4 have been associated with pandemics, with each variant circulating for 3 to 8 years. The Sydney_2012 variant was first reported in Australia and then in other countries. A new variant, GII.P16-GII.4, was recently described in Japan and South Korea and then in the USA, France, Germany and England. In our study, 190 faecal specimens were collected from children admitted to a paediatric hospital and a public health facility during a surveillance study of sporadic cases of AGE conducted between January 2015 and July 2016. The norovirus was detected by RT-qPCR in 51 samples (26.8%), and in 37 of them (72.5%), the ORF1-2 junction was successfully sequenced. The new recombinant GII.P16-GII.4 Sydney was revealed for the first time in Brazil in 2016 and predominated among other strains (9 GII.Pe-GII.4, 3 GII.P17-GII.17, 1 GII.Pg-GII.1, 1 GII.P16-GII.3 and 1 GII.PNA-GII.4). The epidemiological significance of this new recombinant is still unknown, but continuous surveillance studies may evaluate its impact on the population, its potential to replace the first recombinant GII.Pe-GII.4 Sydney 2012 variant, and the emergence of new recombinant forms of GII.P16.
Assuntos
Norovirus/genética , Recombinação Genética , Sequência de Aminoácidos , Brasil/epidemiologia , Infecções por Caliciviridae/epidemiologia , Criança , Pré-Escolar , Humanos , Lactente , Norovirus/classificação , Filogenia , Homologia de Sequência de Aminoácidos , Proteínas Virais/químicaRESUMO
INTRODUCTION: We investigated autochthonous canine visceral leishmaniasis (CVL) in the metropolitan region of Vitória (MRV), an area in which a human case was previously reported. METHODS: Serological, parasitological, and molecular tests were performed in 201 dogs. RESULTS: Twenty-six (13%) and 12 (6%) dogs were identified as positive using in-house enzyme-linked immunosorbent assay (ELISA) and rK39 tests, respectively. Two dogs had a positive culture for Leishmania chagasi, and 4 were polymerase chain reaction (PCR)-positive for Leishmania spp. One positive dog belonged to the aforementioned patient. CONCLUSIONS: Although the responsible vector was not found, our results provide evidence of autochthonous CVL in the MRV, a non-endemic area for VL.
INTRODUÇÃO: Descrevemos um foco de leishmaniose visceral canina (LVC) autóctone na Região Metropolitana de Vitória (RMV) onde um caso humano foi registrado anteriormente. MÉTODOS: Testes sorológicos, parasitológicos e moleculares foram realizados em 201 cães. RESULTADOS: Vinte e seis (13%) e 12 (6%) foram positivos para um teste ELISA in house e rK39, respectivamente. Dois cães apresentaram cultura positiva para Leishmania (Leishmania) chagasi e quatro PCR positivo para Leishmania spp. Um dos cães positivo pertencia ao paciente supracitado. CONCLUSÕES: Embora o vetor não tenha sido encontrado, nossos resultados fornecem evidências da LVC autóctone na RMV, área não-endêmica para leishmaniose visceral.
Assuntos
Animais , Cães , Feminino , Humanos , Masculino , Anticorpos Antiprotozoários/sangue , DNA de Protozoário/sangue , Doenças do Cão/epidemiologia , Leishmaniose Visceral/veterinária , Brasil/epidemiologia , Doenças do Cão/sangue , Ensaio de Imunoadsorção Enzimática/veterinária , Insetos Vetores , Leishmaniose Visceral/epidemiologia , Psychodidae , Reação em Cadeia da Polimerase/veterinária , População UrbanaRESUMO
INTRODUCTION: We investigated autochthonous canine visceral leishmaniasis (CVL) in the metropolitan region of Vitória (MRV), an area in which a human case was previously reported. METHODS: Serological, parasitological, and molecular tests were performed in 201 dogs. RESULTS: Twenty-six (13%) and 12 (6%) dogs were identified as positive using in-house enzyme-linked immunosorbent assay (ELISA) and rK39 tests, respectively. Two dogs had a positive culture for Leishmania chagasi, and 4 were polymerase chain reaction (PCR)-positive for Leishmania spp. One positive dog belonged to the aforementioned patient. CONCLUSIONS: Although the responsible vector was not found, our results provide evidence of autochthonous CVL in the MRV, a non-endemic area for VL.
