RESUMO
Members of the genus Armillaria are widespread forest pathogens against which effective protection has not yet been developed. Due to their longevity and the creation of large-scale cloning of Armillaria individuals, the use of mycoviruses as biocontrol agents (BCAs) against these pathogens could be an effective alternative. This work describes the detection and characterization of viruses in Armillaria spp. collected in the Czech Republic through the application of stranded total RNA sequencing. A total of five single-stranded RNA viruses were detected in Armillaria ostoyae and A. cepistipes, including viruses of the family Tymoviridae and four viruses belonging to the recently described "ambivirus" group with a circular ambisense genome arrangement. Both hammerhead (HHRz) and hairpin (HpRz) ribozymes were detected in all the ambiviricot sequences. Armillaria viruses were compared through phylogenetic analysis and confirmed their specific host by direct RT-PCR. One virus appears to infect both Armillaria species, suggesting the occurrence of interspecies transmission in nature.
Assuntos
Armillaria , Micovírus , Genoma Viral , Filogenia , RNA Viral , República Tcheca , Armillaria/genética , Armillaria/virologia , Micovírus/classificação , Micovírus/genética , Micovírus/isolamento & purificação , RNA Viral/genética , Vírus de RNA/genética , Vírus de RNA/classificação , Vírus de RNA/isolamento & purificação , Doenças das Plantas/virologia , Doenças das Plantas/microbiologia , Análise de Sequência de RNARESUMO
Letter to the editor (No abstract) Keywords.
Assuntos
Armillaria , Micovírus , Armillaria/genética , Micovírus/genética , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Letter to the editor (no abstract) Keywords.
Assuntos
Micovírus , Armillaria , Florestas , Micovírus/genética , FungosRESUMO
Chytridiopsis typographi Weiser, 1954, the microsporidian pathogen of the spruce bark beetle, Ips typographus L. (Coleoptera: Scolytidae), has an early developmental period with plurinucleate mother cells, each of which produces a single bud. The globular bud is connected with the mother cell by a collar and the cellular constituents are pushed to the distant end of the bud. Both the mother cell and the bud continue to develop; the bud then separates from the mother cell and grows to produce a cell of the same type. Both cells then continue sporogonial development and produce sporophorous vesicles with 16-32 spores. The process of a single mother cell producing a single bud that grows to an identical stage is new in the development of C. typographi and has no analogy in other Microsporidia.
