Influence of protein nutrition and virginiamycin supplementation on feedlot growth performance and digestive function of calf-fed Holstein steers.

Two experiments were conducted to examine the influence of protein and virginiamycin (VM) supplementation on feedlot growth performance, digestion, and metabolizable AA (MAA) supply of calf-fed Holstein steers. Growth performance and dietary energetics were evaluated in 120 Holstein steers (127 ± 9 kg). During the initial 112-d feeding period, a steam-flaked corn-based diet was balanced to meet either 100% (MAB) or 87% (UREA) of MAA requirements. Diets were supplemented with or without 22.5 mg/kg VM in a 2 × 2 factorial arrangement. Subsequently (d 112 to 308), all steers received the UREA diet with or without VM. During the initial 112-d, MAB increased ADG, G:F, and dietary NE ( < 0.01). Thereafter, when all steers received the UREA diet, ADG, G:F, and dietary NE were not different ( > 0.10) across initial supplementation treatments. Overall (d 1 to 308), MAB did not affect ADG ( > 0.10) but enhanced G:F efficiency ( = 0.03) and dietary NE ( = 0.05). During the initial 112-d period and through the remainder of the experiment, VM increased G:F ( < 0.01) and dietary NE ( < 0.01). Four Holstein steers (146 ± 4 kg) with cannulas in the rumen and proximal duodenum were used in a 4 × 4 Latin square design to evaluate initial 112-d treatment effects on digestive function. There were no treatment effects ( > 0.10) on ruminal digestion of OM, NDF, starch, microbial efficiency, or total tract digestion of OM and NDF. The MAB increased indispensable AA flow to the small intestine ( < 0.01) and total tract digestion of N ( < 0.01) and starch ( = 0.04). Observed AA supply to small intestine was in agreement with expected supply ( = 0.96). Virginiamycin decreased ( = 0.04) nonammonia N flow to the small intestine and did not affect ( > 0.10) total tract N digestion. Extrapolating from AA supplies in the metabolism study, MAB satisfied indispensable AA requirements during the initial 112-d period, whereas the UREA diet met 73.5% and 79.2% of methionine and lysine requirements, respectively. During the subsequent periods (d 112 to 308) indispensable AA supplies exceeded theoretical requirements. We conclude that enhancements in energy utilization when diets are balanced to meet MAA requirements of calf-fed Holstein steers during the initial 112-d feedlot period remain appreciable throughout time on feed. Virginiamycin enhanced efficiency of energy utilization throughout the feedlot growing-finishing period.

Comparative feeding value of distillers dried grains plus solubles as a partial replacement for steam-flaked corn in diets for calf-fed Holstein steers: characteristics of digestion, growth performance, and dietary energetics.

Two experiments were conducted to examine the effect of level of dried distillers grains plus solubles (DDGS) supplementation (0, 10, 20, and 30%; DM basis), replacing steam-flaked (SF) corn in finishing diets, on characteristics of digestion (Exp. 1) and growth performance (Exp. 2) in calf-fed Holstein steers. In Exp.1, 4 cannulated Holstein steers (349 ± 12 kg) were used to evaluate treatment effects on characteristics of digestion. Ruminal NDF digestion tended to increase (quadratic effect, P = 0.09) and ruminal OM digestion decreased (linear effect, P = 0.01) with DDGS substitution. There were no treatment effects on duodenal flow of microbial N (MN). Substitution with DDGS increased (linear effect, P < 0.01) N flow to the small intestine. The undegradable intake protein (UIP) value of DDGS was 35%. Postruminal digestion of OM (linear effect, P = 0.04) and fatty acids (linear effect, P = 0.03) and total tract digestion of OM and GE decreased (linear effect, P < 0.03) with increasing level of DDGS substitution. Substitution with DDGS did not affect (P = 0.80) ruminal pH but increased (linear effect, P = 0.01) acetate:propionate molar ratio. In Exp.2, 144 Holsteins steer (112 ± 6 kg) were used in a 305-d trial to evaluate treatment effects on growth performance and carcass characteristics. During the initial 126 d, DDGS substitution increased ADG (linear effect, P = 0.03), G:F (quadratic effect, P = 0.03), and dietary NE (quadratic effect, P = 0.02), maximal for both at 20% DDGS inclusion rate. Based on estimated indispensable AA supply to the small intestine as a percentage of requirements during the initial 126-d period, histidine was first limiting followed by methionine. During the final 179-d period and overall (305-d feeding period), treatment effects on ADG and G:F were small (P ≥ 0.22). Compared with the other treatments, HCW was greater (3.4; P = 0.03) at the 20% level of DDGS substitution. The NE value for DDGS in SF corn-based diets for the calf-fed Holstein are consistent with current tabular standards. Extra-caloric value of DDGS as a metabolizable AA source is apparent during the initial growing phase. The UIP value of DDGS used in this study (35%) was considerably less than current tabular estimates (52%; NRC, 2000).

Influence of Ionophore Supplementation on Growth Performance, Dietary Energetics and Carcass Characteristics in Finishing Cattle during Period of Heat Stress.

Forty-eight crossbred heifers (378.1±18 kg) were used in a 56-d feeding trial (four pens per treatment in a randomised complete block design) to evaluate the influence of ionophore supplementation on growth performance, dietary energetics and carcass characteristics in finishing cattle during a period of heat stress. Heifers were fed a diet based on steam-flaked corn (2.22 Mcal NEm/kg) with and without an ionophore. Treatments were: i) control, no ionophore; ii) 30 mg/kg monensin sodium (RUM30); iii) 20 mg/kg lasalocid sodium (BOV20), and iv) 30 mg/kg lasalocid sodium (BOV30). Both dry matter intake (DMI) and climatic variables were measured daily and the temperature humidity index (THI) was estimated. The maximum THI during the study averaged 93, while the minimum was 70 (THI average = 79.2±2.3). Compared to controls, monensin supplementation did not influence average daily gain, the estimated NE value of the diet, or observed-to-expected DMI, but tended (p = 0.07) to increase (4.8%) gain to feed. Compared to controls, the group fed BOV30 increased (p≤0.03) daily gain (11.8%), gain to feed (8.3%), net energy of the diet (5%), and observed-to-expected DMI (5.2%). Daily weight gain was greater (7.6%, p = 0.05) for heifers fed BOV30 than for heifers fed MON30. Otherwise, differences between the two treatments in DMI, gain to feed, and dietary NE were not statistically significant (p>0.11). Plotting weekly intakes versus THI, observed intake of controls was greater (p<0.05) at THI values ≤77 than ionophore groups. When THI values were greater than 79, DMI of control and MON30 were not different (p = 0.42), although less than that of groups fed lasalocid (p = 0.04). Variation in energy intake was lower (p>0.05) in the ionophores group (CV = 1.7%) than in the control group (CV = 4.5%). Inclusion of ionophores in the diet resulted in relatively minor changes in carcass characteristics. It is concluded that ionophore supplementation did not exacerbate the decline of DM intake in heat-stressed cattle fed a high-energy finishing diet; on the contrary, it stabilised feed intake and favoured feed efficiency. Ionophore supplementation reduced estimated maintenance coefficients around 10% in finishing cattle during a period of heat stress. This effect was greatest for heifers supplemented with 30 mg lasalocid/kg of diet.

Feeding value of dried shredded sugarbeets as a partial replacement for steam-flaked corn in finishing diets for feedlot cattle.

Two experiments were conducted to evaluate the comparative feeding value of dried shredded sugarbeets (DSSB; 0, 20, and 40% of diet DM) as a replacement for steam-flaked corn (SFC) in finishing diets for feedlot cattle. In Exp. 1, 60 calf-fed Holstein steers (476 ± 6.3 kg) were used in a 97-d finishing trial. Substitution of SFC with DSSB did not affect ADG or DMI (P > 0.20). Increasing DSSB decreased gain efficiency (ADG:DMI; linear effect, P = 0.04) and dietary NE (linear effect, P = 0.03). Given that SFC has a NE(m) value of 2.38 Mcal/kg, the replacement NE(m) and NE(g) values for DSSB were 1.94 and 1.29 Mcal/kg, respectively. There were no treatment effects (P > 0.20) on carcass characteristics. In Exp. 2, 6 cannulated Holstein steers (205 kg) were used in a replicated 3 × 3 Latin square design to evaluate treatment effects on digestion. Ruminal digestion of starch, NDF, and feed N were not affected (P > 0.10) by DSSB, although ruminal OM digestion tended to increase (linear effect, P < 0.08). Replacing SFC with DSSB decreased flow of starch to the small intestine, but it increased flow of microbial N (linear effect, P = 0.05). There were no treatment effects (P > 0.14) on postruminal digestion of OM, NDF, starch, or feed N or total tract digestion of OM, starch, and N. Substitution of DSSB increased (linear effect, P = 0.05) total tract NDF digestion and decreased (linear effect, P = 0.05) dietary DE (Mcal/kg). Given that SFC has a DE value of 4.19 Mcal/kg, the replacement DE value of DSSB was 3.68 Mcal/kg. There were no treatment effects (P > 0.12) on ruminal pH or total VFA; however, DSSB decreased propionate (linear effect, P = 0.05) and increased acetate (linear effect, P = 0.07), butyrate (linear effect, P = 0.05), valerate (linear effect, P = 0.04), and estimated methane production (linear effect, P = 0.05). We concluded that DSSB may replace SFC in finishing diets at levels of up to 40% without detrimental effects on ADG and carcass characteristics. The NE value of DSSB is 82% that of SFC (DM basis). Partial replacement of SFC with DSSB alters ruminal VFA patterns, increasing estimated methane energy loss and slightly decreasing the efficiency of DE utilization.

Influence of processing method on comparative digestion of white corn versus conventional steam-flaked yellow dent corn in finishing diets for feedlot cattle.

Four Holstein steers (137 ± 2 kg) with cannulas in the rumen and proximal duodenum were used in a 4 × 4 Latin square design to evaluate the influence of processing method on comparative digestion of white corn. Treatments consisted of a basal finishing diet containing 80% corn grain (DM basis) as 1) dry-rolled white corn (DRWC), 2) steam-flaked white corn (SFWC) with 0.36 kg/L flake density (SFWC36), 3) SFWC, 0.31 kg/L flake density (SFWC31), and 4) steam-flaked yellow corn (SFYC) with 0.31 kg/L flake density (SFYC31). Characteristics of ruminal, postruminal, and apparent total tract digestion of OM, starch, and N were similar (P ≥ 0.08) for SFYC31 and SFWC31 treatments. Decreasing flake density of white corn (from 0.36 to 0.31 kg/L) did not affect (P = 0.22) ruminal OM digestion, but increased (1.9%, P = 0.07) apparent total tract OM digestion. Compared with dry rolling, steam flaking white corn increased ruminal (9.4%, P = 0.05), postruminal (14.4%, P < 0.01), and apparent total tract OM digestion (8.2%, P < 0.01), reflecting corresponding increases in ruminal (13.3%, P < 0.01), postruminal (43%, P < 0.01), and apparent total tract (12.3%, P < 0.01) starch digestion. Apparent postruminal and apparent total-tract N digestion also were greater (6.5 and 5.6%, respectively, P = 0.04) for SFWC than for DRWC. The DE value of SFWC and SFYC diets was similar, averaging 3.39 Mcal/kg. The DE value of SFWC was greater (8.1%, P < 0.01) than that of DRWC. Ruminal pH (4 h postprandial) averaged 5.74 and was not affected (P ≥ 0.48) by dietary treatments. Compared with dry rolling, steam flaking markedly enhances the feeding value of white corn, with optimal flake density being less than 0.36 kg/L. Although white corn has greater vitreous endosperm content, characteristics of ruminal starch digestion and undegradable intake protein are similar to conventional yellow dent corn when processed to a similar flake density (0.31 kg/L). However, postruminal and apparent total tract starch digestion tends to be slightly less for flaked white corn than for yellow corn.

Comparative effects of virginiamycin supplementation on characteristics of growth-performance, dietary energetics, and digestion of calf-fed Holstein steers.

Two trials were conducted to evaluate the influence of virginiamycin supplementation on growth performance and digestive function. In trial 1, 144 Holstein steer calves (119 kg of initial BW) were used in a 340-d trial. Treatments were (DM basis) 1) control (no antibiotic); 2) 16 mg/kg of virginiamycin; 3) 22.5 mg/kg of virginiamycin; and 4) 28 mg/kg of monensin. Calves received a steam-flaked corn-based growing diet for the first 112 d and thereafter were fed a finishing diet from d 112 until slaughter. Steers were divided into 2 BW groups (lighter-half and heavier-half), and assigned within groups to 24 pens (6 steers/pen). Virginiamycin did not affect (P > 0.20) ADG, but increased 340-d G:F (linear; P = 0.02) and dietary NE (linear; P = 0.04). Improvements in dietary NE were most pronounced during the final 116 d of the feeding period (linear; P = 0.04) that comprised the hot summer months (June through September). Monensin did not affect (P > 0.20) growth performance or dietary NE. Although average initial BW of the lighter-half and heavier-half BW groups differed by only 4 kg, the heavier BW group had greater HCW (13 kg, P = 0.02), LM area (5.4%, P = 0.01), ADG (4.3%, P = 0.02), G:F (2.3%, P = 0.08), and dietary NE (3.2%. P = 0.01) than the lighter BW group. In trial 2, 4 Holstein steers (269 +/- 3.5 kg) with cannulas in the rumen and proximal duodenum were used in a 4 x 4 Latin square design to evaluate treatment effects on digestion. Virginiamycin did not affect (P > 0.20) ruminal digestion of OM, NDF, starch, and N, and microbial efficiency, but tended to linearly increase (P = 0.09) ruminal N efficiency (nonammonia N flow to the small intestine/N intake). Likewise, virginiamycin did not affect (P > 0.20) postruminal or total tract digestion of OM, starch, NDF, and N. Compared with the control diet, monensin depressed total tract digestion of OM (4.2%, P = 0.03) and NDF (29.5%, P = 0.02). There were no treatment effects (P > 0.20) on ruminal pH, or ruminal lactate and total VFA molar concentration. Virginiamycin increased ruminal molar proportion of acetate (quadratic; P = 0.04) and estimated methane production (quadratic; P = 0.07), and decreased propionate molar proportion (quadratic; P = 0.09). Monensin did not affect (P > 0.20) ruminal VFA molar proportions or estimated methane production. We concluded that virginiamycin supplementation can enhance feedlot growth-performance and dietary energetic efficiency of calf-fed Holstein steers.

Effects of preslaughter withdrawal period on response of feedlot heifers to zilpaterol hydrochloride supplementation: growth performance and carcass characteristics.

Sixty-four crossbred heifers (451 +/- 23 kg) were used in a 42-d feeding trial (4 pens per treatment in a randomized complete block design) to evaluate the influence of preslaughter zilpaterol hydrochloride withdrawal period on growth performance and carcass characteristics. Heifers were fed a diet based on steam-flaked corn (2.13 Mcal of NE(m)/kg). Treatments were 1) control, no zilpaterol supplementation; 2) zilpaterol supplementation for 30 d, drug withdrawn from the diet 3 d preslaughter (ZIL-3); 3) zilpaterol supplementation for 30 d, drug withdrawn 6 d preslaughter (ZIL-6), and 4) zilpaterol supplementation for 30 d, drug withdrawn 12 d preslaughter (ZIL-12). Zilpaterol was supplemented at the rate of 0.15 mg/kg of BW daily. Intake of DM averaged 9.2 +/- 0.26 kg/d and was not affected (P > or = 0.36) by treatment. Compared with control heifers, ZIL-3 increased (P < 0.01) carcass-adjusted ADG (59%), G:F (57%), apparent dietary NE(m) (31%), and decreased observed/expected DMI (25%). Treatment with ZIL-3 did not affect marbling score (P = 0.49) or backfat thickness (P = 0.17), but compared with the control group, increased HCW (3.6%, P = 0.03), carcass dressing percentage (3.2%, P = 0.02), LM area (6.3%, P = 0.05), and reduced trimmed fat (31%, P = 0.03). Prolonging the period of zilpaterol withdrawal preslaughter tended to decrease carcass-adjusted ADG (linear, P = 0.11), G:F (linear, P = 0.08), apparent dietary NE(m) (linear, P = 0.11), and carcass dressing percentage (linear, P = 0.11). We conclude that growth performance and carcass yield responses to zilpaterol supplementation are negatively affected by prolonging the period of zilpaterol withdrawal beyond 3 d (the required minimum withdrawal period according to label). Drug withdrawal period may be a relevant factor in explaining variation in performance response to zilpaterol supplementation in commercial feedlots.

The effect of feeding low-phytate barley-soybean meal diets differing in protein content to growing pigs on the excretion of phosphorus and nitrogen.

An experiment was conducted with growing pigs to determine the excretion of P and N in 4 barley-based diets formulated to contain 18 or 15% CP by using a normal barley (NB) or a low-phytate barley (LPB). The NB contained 0.31% total P and 0.19% phytate P; the LPB contained 0.32% total P and 0.01% phytate P. The diets were supplemented, when so required, with lysine, methionine, threonine, and tryptophan to meet their apparent ileal digestible supplies according to the NRC (1998). The diets containing NB were supplemented with inorganic P to meet the NRC (1998) recommendation for available P (0.23%). The diets containing LPB were not supplemented with inorganic P because these contained sufficient available P (0.27%). Eight barrows with an average BW of 20.9 kg were assigned to the 4 dietary treatments according to a repeated 4 x 4 Latin square design. The diets were fed at a rate of 2.5 times the ME requirement for maintenance. The barrows were fed twice daily, at 0800 and 1500, equal amounts each meal. Water was added to the feed at a ratio of 2.5:1. Each experimental period consisted of a 7-d adaptation period followed by a 5-d collection of feces and urine. The substitution of NB with LPB decreased (P < 0.001) the total P excretion by 38 and 43% for the 18 and 15% CP diets, respectively. Reducing the CP content from 18 to 15% decreased (P < 0.001) the N excretion by 29 and 32% for the NB and LPB diets, respectively. With the reduction in CP content, there was a decrease (P < 0.001) in the amount of N retained. The N:P ratio in manure of pigs fed the LPB diets was greater (P < 0.001) than from pigs fed the NB diets. These data indicate that P and N excretion can be greatly reduced by substitution of NB by LPB, and also by the reduction of the CP content, in diets for growing pigs.

Ileal amino acid digestibility and performance of growing pigs fed wheat-based diets supplemented with xylanase.

Two experiments were conducted to determine the effect of supplementation of xylanase to a wheat-based diet on the apparent ileal digestibility (AID) of AA and the performance of growing pigs fed diets limiting in AA. In Exp. 1, eight pigs (average initial BW = 20.5+/-1.2 kg) fitted with a simple T-cannula at the distal ileum, were fed four diets according to a repeated 4 x 4 Latin square design. Diet 1 was a basal diet that contained 97.6% wheat. Diets 2, 3, and 4 were the basal diet supplemented with xylanase at rates of 5,500, 11,000, and 16,500 units of xylanase activity (XU), respectively (as-fed basis). There were linear and quadratic effects (0.062 < P < 0.001) of xylanase supplementation on the AID of CP and most of the AA. The largest increases in AID of CP and AA were obtained when xylanase was supplemented at a rate of 11,000 XU; no further increases were observed with xylanase supplementation at a rate of 16,500 XU. In Exp. 2, 30 pigs (average initial BW 21.4+/-1.8 kg) were randomly allotted to six dietary treatments. Diets 1 to 4 were similar to those used in Exp. 1. Diet 5 was the same as Diet 1, but supplemented with 0.53% lysine, 0.12% threonine, and 0.05% methionine. Diet 6 (positive control diet) was a wheat-soybean meal diet that contained 18.2% CP (as-fed basis). The total contents of lysine, threonine, and methionine were similar for Diets 5 and 6. There was a linear effect of xylanase supplementation on ADG (P = 0.093) and feed:gain ratio (P = 0.089), and a quadratic effect on ADG (P = 0.067) and feed:gain ratio (P = 0.074). But, the greatest response was obtained with the supplementation of 11,000 XU. The supplementation of lysine, threonine, and methionine to Diet 1 increased (P = 0.001) ADG and ADFI and improved (P = 0.01) feed:gain ratio. There was no difference (P = 0.508) in the performance of pigs fed the AA-supplemented or control diet. In conclusion, the supplementation of xylanase to a diet in which wheat provided the sole source of protein and energy improved the AID of AA, ADG, and feed:gain ratio; however, this improvement was very small compared with that obtained with the supplementation of synthetic amino acids.