[Fluconazole susceptibility of invasive Candida sp isolates as determined by three methods: Bogotá - Colombia]. / Evaluación mediante tres técnicas de susceptibilidad a fluconazol en especies de Candida aisladas en pacientes con infecciones invasoras: Bogotá - Colombia.

UNLABELLED: The frequency of invasive mycosis caused by Candida sp has increased significantly in immuno-compromised patients and in those under medical/surgical treatment; along with it, the use of azoles has fostered resistance. OBJECTIVE: To compare in vitro susceptibility techniques, susceptibility to fluconazol was determined by the M27-A2 method of CLSI (Clinical Laboratory Standard Institute), the disk diffusion technique in 90 invasive Candida sp isolated species (49 C albicans, 30 C. tropicalis, 5 C parapsilosis, 4 C . guilliermondii and 2 C. lusitaniae) and the E-test in 16 isolated. RESULTS: Over 90% of strains were susceptible to fluconazol. A straight correlation between disk-diffusion technique and the E-test was observed regarding the reference method (0.944 and 0.938, respectively). CONCLUSION: In our institution the use of fluconazol for invasive candidiasis is recommended, as well as the routine use of the disk-diffusion or E-test techniques in high complex hospitals in Colombia.

Evaluación mediante tres técnicas de susceptibilidad a fluconazol en especies de Candida aisladas en pacientes con infecciones invasoras: Bogotá - Colombia / Fluconazole susceptibility of invasive Candida sp isolates as determined by three methods: Bogotá - Colombia

The frequency of invasive mycosis caused by Candida sp has increased significantly in immuno-compromised patients and in those under medical/surgical treatment; along with it, the use of azoles has fostered resistance. Objective: To compare in vitro susceptibility techniques, susceptibility to fluconazol was determined by the M27-A2 method of CLSI (Clinical Laboratory Standard Institute), the disk diffusion technique in 90 invasive Candida sp isolated species (49 C albicans, 30 C. tropicalis, 5 C parapsilosis, 4 C . guilliermondii and 2 C. lusitaniae) and the E-test in 16 isolated. Results: Over 90 percent of strains were susceptible to fluconazol. A straight correlation between disk-diffusion technique and the E-test was observed regarding the reference method (0.944 and 0.938, respectively). Conclusión: In our institution the use of fluconazol for invasive candidiasis is recommended, as well as the routine use of the disk-diffusion or E-test techniques in high complex hospitals in Colombia.

La frecuencia de micosis invasoras causadas por Candida sp en pacientes inmunocomprometidos o con tratamientos médico-quirúrgicos ha aumentado y con ello, el uso de azoles, facilitando la aparición de resistencia. Objetivo: Comparar tres métodos de detección de susceptibilidad in vitro a fluconazol: el método de referencia M27-A2 del CLSI (Clinical Laboratory Standard Institute), técnica de difusión en disco a 90 aislados obtenidos en procesos infecciosos invasores (49 C. albicans; 30 C. tropicalis; 5 C. parapsilosis; 4 C. guilliermondii y 2 C. lusitaniaé) y la epsilometría (E-test®) en 16 aislados. Resultados: La susceptibilidad fue mayor a 90 por ciento. Se encontró un buen porcentaje de concordancia entre las técnicas de difusión de disco y E-test® con respecto al método de referencia (0,944 y 0,938, respectivamente). Conclusión: En nuestra institución se considera de elección fluconazol para tratar infecciones micóticas invasoras y recomendamos las técnica de difusión en disco o E-test® para determinar la susceptibilidad en la rutina en hospitales de alta complejidad en Colombia.