RESUMO
Transsynaptic regulation is one mechanism that controls expression of several calmodulin (CaM)-dependent enzymes. This observation and the demonstration that expression of several CaM-dependent enzymes in developing striatum occurred with a spatial and temporal pattern similar to that seen for dopamine and tyrosine hydroxylase suggested that the nigrostriatal pathway may influence the expression of CaM-binding proteins (CaM-BPs) during striatal development. Therefore, the possible role of nigrostriatal dopamine systems regulating the expression of CaM-dependent enzymes was studied in Sprague-Dawley rats by using surgical hemitransections of brain, 6-hydroxydopamine lesions, and chronic haloperidol treatments. Alterations in CaM-BP expression following perturbation of the developing nigrostriatal tract were analyzed by using immunoblots, biotinylated CaM overlays, and enzyme assays. The extent of nigrostriatal lesions was assessed by using depletion of immunoreactive tyrosine hydroxylase levels in striatum. All three experimental paradigms failed to alter the normal developmental expression of CaM-dependent enzymes. From these results we conclude that the increased expression of CaM-dependent enzymes during striatal development is not directly dependent on synaptic input from the nigrostriatal dopamine system.
