RESUMO
Iron dysregulation is implicated in numerous diseases, and iron homeostasis is profoundly influenced by the labile iron pool (LIP). Tools to easily observe changes in the LIP are limited, with calcein AM-based assays most widely used. We describe here FlCFe1, a ratiometric analogue of calcein AM, which also provides the capacity for imaging iron in 3D cell models.
Assuntos
Neoplasias Colorretais/metabolismo , Fluoresceínas/metabolismo , Corantes Fluorescentes/metabolismo , Quelantes de Ferro/metabolismo , Ferro/metabolismo , Esferoides Celulares/metabolismo , Neoplasias Colorretais/patologia , Cumarínicos/química , Fluoresceínas/química , Corantes Fluorescentes/química , Humanos , Ferro/análise , Esferoides Celulares/patologia , Células Tumorais CultivadasRESUMO
The effect of antiretroviral therapy on the natural history of human papillomavirus (HPV)-associated genital lesions was evaluated in 201 human immunodeficiency virus (HIV)-infected women who were followed-up for 1-6 years. Gynecologic examinations were performed every 6-12 months. HPV sequences in cervico-vaginal cells, analyzed by polymerase chain reaction and typed by restriction fragment-length polymorphism analysis, were repeatedly detected in 126 women; 29 had transient HPV infection. Genital lesions were found in 137 patients; prevalence was comparable in women who were receiving different antiretroviral regimens. Regression of low-grade lesions was more prevalent among patients receiving highly active antiretroviral therapy than among those receiving other regimens; high-grade lesions regressed in the majority of cases, regardless of antiretroviral therapy. HPV infection persisted in nearly 80% of the cases. In conclusion, our data show that antiretroviral therapy does not prevent the development of HPV-associated lesions and does not eliminate HPV infection; therefore, early and strict gynecologic follow-up of HIV-infected women is warranted.
Assuntos
Doenças dos Genitais Femininos/patologia , Infecções por Papillomavirus/patologia , Adulto , Idoso , Terapia Antirretroviral de Alta Atividade , Antivirais/uso terapêutico , Feminino , Doenças dos Genitais Femininos/tratamento farmacológico , Doenças dos Genitais Femininos/virologia , Infecções por HIV/complicações , Infecções por HIV/patologia , Soropositividade para HIV , Humanos , Pessoa de Meia-Idade , Papillomaviridae/efeitos dos fármacos , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/tratamento farmacológico , Infecções por Papillomavirus/virologiaRESUMO
Prostaglandin synthesis inhibition have been proposed as an effective alternative to prevent preterm labour. This case report shows a pregnancy with anhydramnios and maternal thrombocytopenia, as side effects of the nimesulide therapy.
