COVID-19 after two years: trajectories of different components of mental health in the Spanish population.

AIMS: Our study aimed to (1) identify trajectories on different mental health components during a two-year follow-up of the COVID-19 pandemic and contextualise them according to pandemic periods; (2) investigate the associations between mental health trajectories and several exposures, and determine whether there were differences among the different mental health outcomes regarding these associations. METHODS: We included 5535 healthy individuals, aged 40-65 years old, from the Barcelona Brain Health Initiative (BBHI). Growth mixture models (GMM) were fitted to classify individuals into different trajectories for three mental health-related outcomes (psychological distress, personal growth and loneliness). Moreover, we fitted a multinomial regression model for each outcome considering class membership as the independent variable to assess the association with the predictors. RESULTS: For the outcomes studied we identified three latent trajectories, differentiating two major trends, a large proportion of participants was classified into 'resilient' trajectories, and a smaller proportion into 'chronic-worsening' trajectories. For the former, we observed a lower susceptibility to the changes, whereas, for the latter, we noticed greater heterogeneity and susceptibility to different periods of the pandemic. From the multinomial regression models, we found global and cognitive health, and coping strategies as common protective factors among the studied mental health components. Nevertheless, some differences were found regarding the risk factors. Living alone was only significant for those classified into 'chronic' trajectories of loneliness, but not for the other outcomes. Similarly, secondary or higher education was only a risk factor for the 'worsening' trajectory of personal growth. Finally, smoking and sleeping problems were risk factors which were associated with the 'chronic' trajectory of psychological distress. CONCLUSIONS: Our results support heterogeneity in reactions to the pandemic and the need to study different mental health-related components over a longer follow-up period, as each one evolves differently depending on the pandemic period. In addition, the understanding of modifiable protective and risk factors associated with these trajectories would allow the characterisation of these segments of the population to create targeted interventions.

The potential of a light spot, heat area, and novel object to attract laying hens and induce piling behaviour.

Piling behaviour of laying hens often results in smothering or death due to suffocation. Mechanisms leading to piling are not yet understood though various potential factors have been suggested. In this experimental study, we predicted that the presence of a light spot, a novel object (metal foil), or a heat area within animal pens would increase animal numbers around the stimulus leading to piling behaviour. We presented the cues in a 4 × 2 Latin-square design in eight identical experimental pens including each 55 Lohmann Selected Leghorn hens. The cues were presented in two test areas per pen, at two bouts per day in the morning, consecutively for 5 days, over four periods (age: 20, 22, 24, 26 weeks). Each pen received a cue and control condition simultaneously (test areas without cue presentation) once. For a bout, each cue was presented for 35 min except for the light spot where the duration was 10 min. Birds' responses to the cues during bout and non-bout times were video recorded and analysed for the first bout of each period. To assess the cues' attractiveness, the number of hens during bout times was counted at predefined times within the test and control areas. To assess the cues' effects on piling, we described piling behaviour (pile number, duration, animal numbers, trigger) in control and test areas during bout times. Furthermore, we described piling behaviour during bout times and non-bout times on the first day of the first period and fourth period. The best model explaining the number of hens included the interactions of treatment and bout time, and treatment and area. Over the bout's time course, more hens were attracted to the light spot compared to the control condition, and more to test areas compared to control areas. In the novel object condition, more hens were drawn to the test areas compared to the control areas. Hens were not attracted to the heat area. Piling in bout times was observed twice when hens pecked at the novel object. During non-bout times, piling behaviour occurred frequently at midday and in the late morning compared to the afternoon, mostly in corners and mainly preceded by the mutual attraction of hens. Overall, hens were attracted to light spots and less so to the novel object though neither reliably induced piling behaviour. The occurrence of piling behaviour in non-bout times shows that more work is needed to understand mechanisms eliciting piling behaviour.

The role of the Sapienza GLObal Bedside Evaluation of Swallowing after Stroke (GLOBE-3S) in the prevention of stroke-associated pneumonia (SAP).

BACKGROUND AND PURPOSE: Stroke-associated pneumonia (SAP) affects 10 to 38% of patients in the acute phase of stroke. Stroke patients diagnosed with dysphagia have an 11-fold higher risk of developing SAP. Thus, identifying dysphagic patients through a highly accurate screening tool might be crucial in reducing the incidence of SAP. We present a case-control study designed to evaluate efficacy in reducing the risk of SAP between two swallowing screening tools, the classic water swallow test (WST) and a recently validated tool such as the GLOBE-3S (the Sapienza GLObal Bedside Evaluation of Swallowing after Stroke), which is a highly sensitive swallowing screening tool particularly accurate in detecting silent aspiration as well. METHODS: We analyzed the occurrence of dysphagia in 100 acute stroke patients distributed in two groups: half were screened with WST and the other half with GLOBE-3S. RESULTS: Dysphagia was diagnosed in 28 patients. The main result is that, among patients who passed the dysphagia screenings, none of those screened with the GLOBE-3S method developed pneumonia compared to 31.82% in the WST group. Discriminant function analysis (DFA) showed that NIH Stroke Scale (NIHSS) score and the dysphagia screening method (i.e., GLOBE-3S vs. WST) were the two main factors in the SAP's predicting model and the only significant ones per se. CONCLUSIONS: The new GLOBE-3S screening test can reduce the risk of SAP compared to WST.

Influence of a raised slatted area in front of the nest on leg health, mating behaviour and floor eggs in broiler breeders.

European farms for broiler breeders often have raised slatted areas in front of the nests, but in other regions of the world no raised slatted areas are provided. This study aimed to investigate the effects of a raised slatted area on leg health, mating behaviour and floor laying behaviour. Ten groups of 33 broiler breeder hens and three males were housed in two pen types: with or without a raised slatted area in front of the nests. Each pen had one plastic and one wooden nest. Between 25 and 31 weeks of age, ten marked hens per pen were weighed and assessed weekly on foot pad dermatitis, hock burn and wounds. At the end of week 31, animals were euthanized and bone strength of the tibia and humerus of these individuals was assessed. At 24, 27 and 30 weeks of age, mating behaviour was observed for an hour per pen, noting both numbers of successful and unsuccessful copulations. The number of eggs laid in the nests and on the floor was recorded daily between 20 and 31 weeks of age. Foot pad dermatitis scores were affected by age, but not by pen type. Generally, there were only minor issues with foot pad dermatitis (scores <11 on a 0-100 scale), probably due to the young age of the hens. Body weight was not affected by pen type, while the prevalence of hock burns was too low to analyse and no difference in bone strength was found for the tibia and the humerus. Overall, mating behaviour was less frequent in pens with raised slats than in pens without raised slats (29 ±â€¯2 vs 35 ±â€¯3 times/h) and more frequent at 27 weeks of age than at 24 and 30 weeks of age (38 ±â€¯1 vs 31 ±â€¯4 and 27 ±â€¯2 times/h). The pens with raised slats had a lower percentage of floor eggs than pens without raised slats (11.2 ±â€¯0.4 vs 19.3 ±â€¯0.5%). The wooden nest was preferred over the plastic nest as on average 63% of the eggs were laid in the wooden nest. This study shows that providing raised slats decreases mating behaviour and percentage of floor eggs, although its effects on leg health remain inconclusive.

Mexican population sub-analysis of the lixilan clinical program with the fixed ratio combination of insulin glargine and lixisenatide (iGlarLixi).

AIM: To evaluate the efficacy and safety of iGlarLixi in Mexican patients with type 2 diabetes who participated in the LixiLan clinical trials and compare results with the rest of the patients. METHODS: Data was collected for Mexican patients who participated in either of three studies: phase 2 trial LixiLan-POC, that compared iGlarLixi vs insulin glargine (iGlar) on inadequately controlled patients with metformin; phase 3 trial LixiLan-O, comparing iGlarLixi vs iGlar and lixisenatide on inadequately controlled patients with oral antidiabetic agents; and finally the phase 3 trial LixiLan-L, comparing iGlarLixi vs iGlar on inadequately controlled patients with basal insulin. The primary endpoint was the change in HbA1c from baseline to end of treatment. RESULTS: In the Mexican population, treatment with iGlarLixi significantly improved HbA1c compared with each component alone achieving an average of 6.5%; (6.17%, 6.63% and 6.73% for the LixiLan-POC, O and L studies respectively) and an average HbA1c reduction from baseline of 1.6%, for the three studies at end of treatment period. CONCLUSION: The efficacy and safety profile of iGlarLixi demonstrate a fair or better composite endpoint of HbA1c without hypoglycemia and no weight gain compared to overall trial population, which could help improve Mexican patients' outcomes.

Influence of later exposure to perches and nests on flock level distribution of hens in an aviary system during lay.

Aviaries provide hens with many resources, but birds must develop motor and cognitive skills to use them properly. Introducing birds to aviaries at older ages has been reported to result in less use of perches, nests, and vertical space, which can reduce productivity and hen welfare. The objectives of this study were to examine (1) how enrichment influenced distribution of hens in the aviary during the day and (2) how enrichment influenced the distribution and roosting substrate of birds at night. Hy-Line W36 pullets were raised in floor pens before moving to laying aviaries (100 hens/aviary unit × 4 units/treatments). Control (CON) pullets were placed into aviaries at 17 wk of age (WOA). Floor (FLR) and enriched (ENR) pullets remained in floor pens until 25 WOA, and ENR birds were provided with perches and nests at 17 WOA. Birds were counted in tiers and litter areas of the aviary at morning, midday and evening at 36 and 54 WOA. Data were analyzed using generalized linear mixed models in R statistical software. At 36 WOA, ENR and CON birds occupied aviary areas at similar rates but differently from FLR birds. For example, in the morning 34% of CON hens and 30% of ENR hens occupied the highest tier compared to 15% of FLR hens (P < 0.01). At midday, 57% of CON and 57% of ENR birds were counted in litter compared with 77% of FLR birds (P < 0.01). In the evening, CON and ENR hens moved to the top tier of the aviary in greater numbers than FLR hens (22 and 17%, respectively, vs. 7%, P < 0.01). At 54 WOA, differences between FLR hens and CON/ENR hens were less pronounced, suggesting FLR hens were adapting to the aviary. Overall, we conclude that birds exposed to aviaries at 25 WOA can adapt to aviary systems, but take more time to do so than birds exposed to aviaries or vertical enrichment at 17 WOA.

Keel bone fractures induce a depressive-like state in laying hens.

In commercial flocks of laying hens, keel bone fractures (KBFs) are prevalent and associated with behavioural indicators of pain. However, whether their impact is severe enough to induce a depressive-like state of chronic stress is unknown. As chronic stress downregulates adult hippocampal neurogenesis (AHN) in mammals and birds, we employ this measure as a neural biomarker of subjective welfare state. Radiographs obtained longitudinally from Lohmann Brown laying hens housed in a commercial multi-tier aviary were used to score the severity of naturally-occurring KBFs between the ages of 21-62 weeks. Individual birds' transitions between aviary zones were also recorded. Focal hens with severe KBFs at 3-4 weeks prior to sampling (n = 15) had lower densities of immature doublecortin-positive (DCX+) multipolar and bipolar neurons in the hippocampal formation than focal hens with minimal fractures (n = 9). KBF severity scores at this time also negatively predicted DCX+ cell numbers on an individual level, while hens that acquired fractures earlier in their lives had fewer DCX+ neurons in the caudal hippocampal formation. Activity levels 3-4 weeks prior to sampling were not associated with AHN. KBFs thus lead to a negative affective state lasting at least 3-4 weeks, and management steps to reduce their occurrence are likely to have significant welfare benefits.

Later exposure to perches and nests reduces individual hens' occupancy of vertical space in an aviary and increases force of falls at night.

Tiered aviaries are intended to improve laying hen welfare by providing resources that enable them to perform essential behaviors. However, hens must be able to navigate these complex systems efficiently and safely. This study investigated the influence of providing perches and nests starting at 17 or 25 wk of age (WOA) on hens' use of vertical space in an aviary at 36 and 54 WOA. Three treatments were applied to pullets raised in floor pens until 17 WOA (4 units/treatment; 100 hens/unit). Control (CON) pullets were placed into aviaries at 17 WOA. Floor (FLR) pullets were placed into aviaries at 25 WOA. Perches and nests were placed in enriched (ENR) pullets' floor pens at 17 WOA prior to moving ENR birds to aviaries at 25 WOA. Five focal hens/unit (n = 20 total hens/treatment) were fitted with accelerometers, and their diurnal movement (g) and frequency (n) and acceleration (g) of falls at night were recorded. Direct observation of focal hens was conducted for 6 min/hen at morning, midday, and evening for 3 consecutive days at 36 and 54 WOA, and location and time spent on vertical tiers were recorded. At 36 WOA, FLR hens spent more time on litter than CON and ENR, which spent more time in the top tier (all P ≤ 0.05). ENR hens exhibited higher vertical movement than CON and FLR hens (0.8, 0.6, and 0.3 g; P = 0.003). CON hens fell most often at night (16 vs. 9 FLR and 5 ENR), whereas FLR hens had higher acceleration and calculated collision force than CON and ENR hens during falls (0.8, 0.5, 0.3 g and 15, 10, 5 N, respectively; P ≤ 0.05). At 54 WOA, hens' movement and vertical distribution were similar across treatments. Delaying birds' access to perches and nests until 25 WOA impacted their movement, vertical space use, and falls at night for at least 10 wk. However, providing perches and nests at 17 WOA, even in floor pens, considerably mitigated such impacts.

Sapienza Global Bedside Evaluation of Swallowing after Stroke: the GLOBE-3S study.

BACKGROUND AND PURPOSE: Dysphagia occurs in up to 50% of all patients with acute stroke. There is debate regarding which is the most effective screening tool in identifying aspiration in patients with acute stroke. We assessed the accuracy of the Sapienza Global Bedside Evaluation of Swallowing after Stroke (GLOBE-3S), which combines the Toronto Bedside Swallowing Screening Test (TOR-BSST©) with oxygen desaturation and laryngeal elevation measurement during swallowing. METHODS: We prospectively enrolled consecutive patients with stroke within 72 h of symptom onset. All patients with stroke firstly underwent a standard neurological examination, then the GLOBE-3S evaluation and finally the fiberoptic endoscopic evaluation of swallowing (FEES). Two different assessors, a neurologist and a speech pathologist, blind to both the clinical data and each other's evaluation, administered the GLOBE-3S and FEES examination. We assessed the accuracy of the GLOBE-3S in detecting post-stroke swallow impairment with aspiration using the FEES as the standard. RESULTS: We enrolled 50 patients with acute stroke, 28 of whom (56%) had swallowing impairment with aspiration at FEES evaluation. A total of 33 patients (66%) failed the GLOBE-3S evaluation. The GLOBE-3S reached a sensitivity of 100% and a specificity of 77.3% (negative predictive value, 100%; positive likelihood ratio, 4.34). The median time required for the GLOBE-3S to be performed was 297 s. CONCLUSIONS: GLOBE-3S is quick to perform at the bedside and can accurately identify aspiration in patients with acute stroke. By including the measurement of laryngeal elevation and monitoring of oxygen desaturation, it could represent a highly sensitive instrument to avoid the misdiagnosis of silent aspirators.

Postoperative intra-abdominal infection is an independent prognostic factor of disease-free survival and disease-specific survival in patients with stage II colon cancer

Background: Recurrence occurs in up to 20% of patients with stage II colon cancer operated on for cure. Although postoperative intra-abdominal infection has been linked with an increased risk of recurrence, the association is controversial. The aim was to investigate the impact of postoperative intra-abdominal infection on disease-free survival and disease-specific survival in patients with stage II colon cancer. Methods: Patients undergoing elective surgery for colon cancer stage II, between 2003 and 2014, were included. Patients with anastomotic leak or intra-abdominal abscess were included in the infection group. We used the Kaplan-Meier method to represent the distribution of survival and the Cox proportional hazards model to estimate the contribution of relevant clinicopathological factors with prognosis. Results: Postoperative intra-abdominal infection was diagnosed in 37 of 363 (10.2%) patients. Perioperative blood transfusion was more frequent in patients with infection (p = 0.008). Overall 5-year disease-free survival rate was 85.1%. Disease-free survival at 5 years was lower in patients with postoperative intra-abdominal infection (52.8 vs 88.7%; p < 0.001), perineural invasion (p = 0.001), lymphovascular invasion (p = 0.001), pT4 (p = 0.013), and in patients with adjuvant chemotherapy (p = 0.013). Multivariate analysis showed that postoperative intra-abdominal infection (HR 4.275; p < 0.001), perineural invasion (HR 2.230; p = 0.007), and lymphovascular invasion (HR 2.052; p = 0.016) were all significant independent predictors of reduced disease-free survival. Regarding specific survival, independent significant prognostic factors were the number of lymph nodes, lymphovascular invasion, and postoperative intra-abdominal infection. Conclusion: In this series of patients with stage II colon cancer, postoperative intra-abdominal infection has an independent negative impact on disease-free survival and disease-specific survival

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Finding hens in a haystack: Consistency of movement patterns within and across individual laying hens maintained in large groups.

We sought to objectively quantify and compare the recorded movement and location patterns of laying hens within a commercial system. Using a custom tracking system, we monitored the location within five zones of a commercial aviary for 13 hens within a flock of 225 animals for a contiguous period of 11 days. Most hens manifested a hen-specific pattern that was (visually) highly consistent across days, though, within that consistency, manifested stark differences between hens. Three different methods were used to classify individual daily datasets into groups based on their similarity: (i) Linear Discriminant Analysis based on six summary variables (transitions into each zone) and total transitions; (ii) Hierarchical Clustering, a naïve clustering analysis technique, applied to summary variables and iii) Hierarchical Clustering applied to dissimilarity matrices produced by Dynamic Time Warping. The three methods correctly classified more than 85% of the hen days and provided a unique means to assess behaviour of a system indicating a considerable degree of complexity and structure. We believe the current effort is the first to document these location and movement patterns within a large, complex commercial system with a large potential to influence the assessment of animal welfare, health, and productivity.

Postoperative intra-abdominal infection is an independent prognostic factor of disease-free survival and disease-specific survival in patients with stage II colon cancer.

BACKGROUND: Recurrence occurs in up to 20% of patients with stage II colon cancer operated on for cure. Although postoperative intra-abdominal infection has been linked with an increased risk of recurrence, the association is controversial. The aim was to investigate the impact of postoperative intra-abdominal infection on disease-free survival and disease-specific survival in patients with stage II colon cancer. METHODS: Patients undergoing elective surgery for colon cancer stage II, between 2003 and 2014, were included. Patients with anastomotic leak or intra-abdominal abscess were included in the infection group. We used the Kaplan-Meier method to represent the distribution of survival and the Cox proportional hazards model to estimate the contribution of relevant clinicopathological factors with prognosis. RESULTS: Postoperative intra-abdominal infection was diagnosed in 37 of 363 (10.2%) patients. Perioperative blood transfusion was more frequent in patients with infection (p = 0.008). Overall 5-year disease-free survival rate was 85.1%. Disease-free survival at 5 years was lower in patients with postoperative intra-abdominal infection (52.8 vs 88.7%; p < 0.001), perineural invasion (p = 0.001), lymphovascular invasion (p = 0.001), pT4 (p = 0.013), and in patients with adjuvant chemotherapy (p = 0.013). Multivariate analysis showed that postoperative intra-abdominal infection (HR 4.275; p < 0.001), perineural invasion (HR 2.230; p = 0.007), and lymphovascular invasion (HR 2.052; p = 0.016) were all significant independent predictors of reduced disease-free survival. Regarding specific survival, independent significant prognostic factors were the number of lymph nodes, lymphovascular invasion, and postoperative intra-abdominal infection. CONCLUSION: In this series of patients with stage II colon cancer, postoperative intra-abdominal infection has an independent negative impact on disease-free survival and disease-specific survival.

Blood-Brain Barrier in a Haemophilus influenzae Type a In Vitro Infection: Role of Adenosine Receptors A2A and A2B.

The blood-brain barrier (BBB) is mainly made up of tightly connected microvascular endothelial cells (BMECs), surrounded by pericytes (BMPCs) which regulate BBB tightness by providing soluble factors that control endothelial proliferation. Haemophilus influenzae type a (Hia) is able to reach the BBB, crossing it, thus causing meningitis. In this study, by using an in vitro model of BBB, performed with human BMECs and human BMPCs in co-culture, we demonstrated that, after Hia infection, the number of hBMPCs decreased whereas the number of hBMECs increased in comparison with non-infected cells. SEM and TEM images showed that Hia was able to enter hBMECs and reduce TEER and VE-cadherin expression. When the cells were infected in presence of SCH58261 and PSB603 but not DPCPX, an increase in TEER values was observed thus demonstrating that A2A and A2B adenosine receptors play a key role in BBB dysfunction. These results were confirmed by the use of adenosine receptor agonists CGS21680, CCPA, and NECA. In infected co-cultures cAMP and VEGF increased and TEER reduction was counter-balanced by VEGF-R1 or VEGF-R2 antibodies. Moreover, the phosphorylated CREB and Rho-A significantly increased in infected hBMECs and hBMPCs and the presence of SCH58261 and PSB603 significantly abrogated the phosphorylation. In conclusion, this study demonstrated that the infection stimulated A2A and A2B adenosine receptors in hBMECs and hBMPCs thus inducing the pericytes to release large amounts of VEGF. The latter could be responsible for both, pericyte detachment and endothelial cell proliferation, thus provoking BBB impairment.

Perch use by broiler breeders and its implication on health and production.

Broiler breeders are commonly kept without perches, although perching has been shown to be a high-priority behavior in laying hens. We studied whether broiler breeders used elevated perches of different lengths during the night and how access to perches affected health and production. Using the Ross 308 hybrid, pens offering 4 different perch spaces per bird (5, 10, 14, and 20 cm) in a cross-over design were compared with pens without perches. The number of birds on perches at midnight were recorded 7 times during production. Prevalence of keel bone fractures, breast blisters, pododermatitis, and plumage quality of hens was assessed at 45 wk, and production was monitored daily. To determine subsequent effects on offspring, chicks from hens with and without perches were reared and their growth rate was assessed. Analysis found more broiler breeders perched at night when 14 cm perch length per bird was provided than with less available perch length (P = 0.0005), but there was no difference between 14 and 20 cm per bird. Perch use declined with age from about 50 to 20% (P < 0.0001). The number of eggs and hatchability were not affected by treatment. During a period of high temperatures, mortality was lower in pens with perches (P = 0.001). Keel bone fractures were present in 1/4 of hens and were not affected by the presence of perches. The growth of chicks was not affected by the parent treatment. In conclusion, our results suggest that perches were chosen for roosting by broiler breeders depending on their age and did not impair production.

Optic Nerve Sheath Diameter Ultrasound Evaluation in Intensive Care Unit: Possible Role and Clinical Aspects in Neurological Critical Patients' Daily Monitoring.

Background. The increase of the optic nerve sheath diameter (ONSD) is a reliable, noninvasive sonographic marker of intracranial hypertension. Aim of the study was to demonstrate the efficacy of ONSD evaluation, when monitoring neurocritical patients, to early identify malignant intracranial hypertension in patients with brain death (BD). Methods. Data from ultrasound ONSD evaluation have been retrospectively analyzed in 21 sedated critical patients with neurological diseases who, during their clinical course, developed BD. 31 nonneurological controls were used for standard ONSD reference. Results. Patients with neurological diseases, before BD, showed higher ONSD values than control group (CTRL: RT 0.45 ± 0.03 cm; LT 0.45 ± 0.02 cm; pre-BD: RT 0.54 ± 0.02 cm; LT 0.55 ± 0.02 cm; p < 0.000) even without intracranial hypertension, evaluated with invasive monitoring. ONSD was further significantly markedly increased in respect to the pre-BD evaluation in neurocritical patients after BD, with mean values above 0.7 cm (RT 0.7 ± 0.02 cm; LT 0.71 ± 0.02 cm; p < 0.000), with a corresponding dramatic raise in intracranial pressure. Logistic regression analysis showed a strong correlation between ONSD and ICP (R 0,895, p < 0.001). Conclusions. ONSD is a reliable marker of intracranial hypertension, easy to be performed with a minimal training. Routine ONSD daily monitoring could be of help in Intensive Care Units when invasive intracranial pressure monitoring is not available, to early recognize intracranial hypertension and to suspect BD in neurocritical patients.

Electrical activity of cellobiose dehydrogenase adsorbed on thiols: Influence of charge and hydrophobicity.

The interface between protein and material surface is of great research interest in applications varying from implants, tissue engineering to bioelectronics. Maintaining functionality of bioelements depends greatly on the immobilization process. In the present study direct electron transfer of cellobiose dehydrogenase from Humicola insolens (HiCDH), adsorbed on four different self-assembled monolayers (SAMs) formed by 5-6 chain length carbon thiols varying in terminal group structure was investigated. By using a combination of quartz crystal microbalance with dissipation, ellipsometry and electrochemistry the formation and function of the HiCDH film was studied. It was found that the presence of charged pyridinium groups was needed to successfully establish direct electron contact between the enzyme and electrode. SAMs formed from hydrophilic charged thiols achieved nearly two times higher current densities compared to hydrophobic charged thiols. Additionally, the results also indicated proportionality between HiCDH catalytic constant and water content of the enzyme film. Enzyme films on charged pyridine thiols had smaller variations in water content and viscoelastic properties than films adsorbed on the more hydrophobic thiols. This work highlights several perspectives on the underlying factors affecting performance of immobilized HiCDH.

Cerebral venous hemodynamic abnormalities in episodic and chronic migraine.

Alterations of cerebral venous drainage have been demonstrated in chronic migraine (CM), suggesting that cerebral venous hemodynamic abnormalities (CVHAs) play a role in this condition. The aim of the present study was to look for a correlation between CM and CVHAs. We recruited 33 subjects suffering from CM with or without analgesic overuse, 29 episodic migraine (EM) patients with or without aura, and 21 healthy subjects as controls (HCs). CVHAs were evaluated by transcranial and extracranial echo-color Doppler evaluation of five venous hemodynamic parameters. CVHAs were significantly more frequent in the CM and EM patients than in the HCs. In the migraine patients, CVHAs were not correlated with clinical features. The significantly greater frequency of CVHAs observed in the migraineurs may reflect a possible relationship between migraine and these abnormalities. Prospective longitudinal studies are needed to investigate whether CVHAs have a role in the processes of migraine chronification.