Murine Hox-1.7 homeo-box gene: cloning, chromosomal location, and expression.

A new murine homeo-box, called Hox-1.7, has been identified in a rare cDNA from F9 teratocarcinoma stem cells. The Hox-1.7 homeo-box is 68 and 72% homologous to the Drosophila antennapedia (Antp) and iab-7 homeo-boxes, respectively. A major 2.5-kilobase transcript and several minor transcripts were detected by Northern blot (RNA blot) analysis in adult tissues as well as in midgestational embryos. The posterior spinal cord was found to be a major site of Hox-1.7 expression in 12.5-day-old embryos. Somatic cell hybrids were used to map the Hox-1.7 gene to mouse chromosome 6. Restriction fragment length polymorphisms associated with either the Hox-1.7 gene or the previously known Hox-1 complex were identified. Their distribution patterns in recombinant inbred mouse strains were used to determine the linkage between the two loci as well as to other loci on chromosome 6. This maps Hox-1 and Hox-1.7 close to two mouse loci that affect morphogenesis, postaxial hemimelia (px) and hypodactyly (Hd).

Region-specific expression of mouse homeobox genes in the embryonic mesoderm and central nervous system.

The homeobox is a 180-base-pair sequence characteristically found in homeotic and segmentation genes in Drosophila. Several copies of homeoboxes are also found in the mammalian genome, but it is not known whether these are components of morphogenetic loci in mammals as well. As a step toward understanding the function of mammalian homeoboxes, we have used in situ hybridization to define the spatial pattern of expression of two mouse homeobox genes in the midgestational mouse embryo. The two mouse homeoboxes studied here, Hox 1.2 and Hox 1.4, are located 20 kilobases apart on mouse chromosome 6. Our results demonstrate the following: (i) Hox 1.2 transcripts are localized mainly in the posterior myelencephalon, in the cervical central nervous system (CNS), and in several thoracic prevertebrae; (ii) Hox 1.4 transcripts are localized mainly in the posterior myelencephalon and in the cervical CNS; (iii) within the CNS region expressing Hox 1.4, the level of Hox 1.4 transcripts is higher in the mantle layer than in the ependymal layer and higher in the dorsal than in the ventral area. The specific localization of Hox 1.2 and Hox 1.4 transcripts in the embryonic CNS and the restricted pattern of expression along the rostrocaudal axis are strikingly reminiscent of the expression pattern of Drosophila homeoboxes in the fly embryo and larvae. Despite the different developmental strategies adopted by Drosophila and mammals, functional similarities may exist between Drosophila and mammalian homeobox genes.

A mouse homeo box gene is expressed in spermatocytes and embryos.

The MH-3 gene, which contains a homeo box that is expressed specifically in the adult testis, was identified and mapped to mouse chromosome 6. By means of in situ hybridization with adult testis sections and Northern blot hybridization with testis RNA from prepuberal mice and from Sl/Sld mutant mice, it was demonstrated that this gene is expressed in male germ cells during late meiosis. In the embryo, MH-3 transcripts were present at day 11.5 post coitum, a stage in mouse development when gonadal differentiation has not yet occurred. The MH-3 gene may have functions in spermatogenesis and embryogenesis.