RESUMO
The relevance of comprehensive studies of the Rapana vital functions is determined by its considerable negative impact on the ecosystem of the Black Sea. The aim of the work was to find out the polymorphism and activity of the main hydrolases in the different parts of the digestive system of Rapana. Hydrolases (proteases, amylases, esterases, lipases and phosphatases) in glandular structures of the Rapana digestive system were studied by electrophoresis. It was found that different sets of hydrolytic enzymes are functioning in certain parts of the Rapana digestive tract. The gland of Leiblein and hepatopancreas played the most important role in the digestion of food components. The salivary glands had the significant influence on proteolysis.
Assuntos
Mucosa Gástrica/enzimologia , Gastrópodes/enzimologia , Expressão Gênica , Hepatopâncreas/enzimologia , Espécies Introduzidas , Glândulas Salivares/enzimologia , Amilases/classificação , Amilases/genética , Amilases/metabolismo , Animais , Mar Negro , Ensaios Enzimáticos , Esterases/classificação , Esterases/genética , Esterases/metabolismo , Gastrópodes/genética , Lipase/classificação , Lipase/genética , Lipase/metabolismo , Peptídeo Hidrolases/classificação , Peptídeo Hidrolases/genética , Peptídeo Hidrolases/metabolismo , Monoéster Fosfórico Hidrolases/classificação , Monoéster Fosfórico Hidrolases/genética , Monoéster Fosfórico Hidrolases/metabolismo , Comportamento Predatório/fisiologiaRESUMO
Penetration of 35S-lipoate into nucleated and nucleus-free erythrocytes, isolated cells (enterocytes) and accumulating tissue preparations from intestinal mucosa was studied in presence or in absence of other vitamins. Effect on this process of overloading the penetration of 35S-lipoate into rat and pigeon erythrocytes was found to be increased; under these conditions the enterocytes bind less 35S-lipoate than in control animals. Water-soluble vitamins (nicotinate, riboflavine, thiamin and, especially, panthotenate) decreased the rate of 35S-lipoate penetration into the cells. The mixture of the vitamins caused less distinct inhibitory effect, than the panthotenate only.
