Depression of glutathione content, elevation of CYP2E1-dependent activation, and the principal determinant of the fasting-mediated enhancement of 1,3-dichloro-2-propanol hepatotoxicity in the rat.

The influence of fasting (18 hours) on the hepatotoxicity of 1,3-dichloro-2-propanol (1,3-DCP) and on various hepatic parameters has been assessed in the rat. Fasting produced an enhancement of the hepatotoxicity which was associated with alterations in a variety of hepatic parameters when measured relative to protein content, most notably glutathione (GSH) levels (decrease) and CYP2E1-mediated enzyme activity (increase), two parameters previously identified as being important determinants to the toxicity. Fasting also decreased the liver weight normalized to body weight. When this was taken into account, total liver CYP2E1-mediated enzyme activity was not significantly altered whereas the total liver GSH level was markedly reduced following fasting. These results imply that the reduction in hepatic GSH is the principal determinant of the enhanced susceptibility to 1,3-DCP hepatotoxicity following fasting.

Cancer of the cervix (stages 1B, 2A and 2B): treatment and results.

Combined methods of treatment of carcinoma of the cervix Stage 1B and 2(A and B) are described using irradiation and surgery. In a personally conducted series of 101 patients, a 5-year survival rate of 86% for Stage 1 and 65.4% for Stage 2 was achieved. The urinary tract morbidity associated with combined methods of treatment and the modifications adopted to prevent it are described. It is concluded that the preferred method of treatment of carcinoma of the cervix Stages 1B, 2A and 2B is irradiation (intracavitary + external megavoltage irradiation) followed by modified radical hysterectomy.