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Mitochondrial DNA A DNA Mapp Seq Anal ; 27(3): 1959-60, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-25329254

RESUMO

Three individual-specific DNA libraries of the deep-sea scleractinian coral Solenosmilia variabilis (Duncan, 1873) were constructed to obtain complete mitochondrial genomes using the 454 Life Science pyrosequencing system. Two mitogenomes were successfully assembled: both were 15,968 bp in length, with base composition of A (24.2%), T (41.1%), C (13.7%) and G (21.0%). The genome contains 13 protein-coding genes, 2 ribosomal RNA genes, 2 transfer RNA genes and a D-loop region. The two mitogenomes were almost identical, with only 5 nucleotide differences (0.03%), including a synonymous substitution within the nad1, nad2 and nad4L genes, and two transversions in the D-loop region. This inter-individual variation indicates that these genes and/or region are potential candidates as molecular markers for population genetic research. The mitogenome of S. variabilis will be useful for future phylogenetic and phylogeographic studies of deep-sea corals.


Assuntos
Antozoários/genética , Variação Genética , Genoma Mitocondrial , Animais , Pareamento de Bases/genética , Sequência de Bases , DNA Mitocondrial/genética , Genes Mitocondriais , RNA Ribossômico/genética , RNA de Transferência/genética
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