Unraveling athletic performance: Transcriptomics and external load monitoring in handball competition.

OBJECTIVE: This study aims to comprehend the impact of handball practice on sub-elite athletes by investigating transcriptomic changes that occur during a match. The primary focus encompasses a dual objective: firstly, to identify and characterize these transcriptomic alterations, and secondly, to establish correlations between internal factors (gene expression), and external loads measured through Electronic Performance and Tracking Systems (EPTS variables). Ultimately, this comprehensive analysis seeks to evaluate both acute and chronic responses to exercise within the context of handball training. METHODS: The study included sixteen elite male athletes from the FC Barcelona handball second team. Blood samples were extracted at three different time points: before the match at baseline levels (T1), immediately upon completion (T2), and 24 hours after completion (T3). Differential gene expression, Gene Ontology Term and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were conducted in two comparisons: Comparison 1 (T1 vs T2) and Comparison 2 (T1 vs T3). Further, the correlation between gene expression levels and training variables (external load) was conducted. RESULTS: In T1 vs T2, 3717 of the 14632 genes detected were differentially expressed (adjusted p-value < 0.05), and enrichment of terms related to the immune system, mitochondria, and metabolic processes was found. Further, significant linear correlations were obtained between High-Speed running (HSR) and high-intensity variables such as acceleration ACC and deceleration DEC values with amino acids, and inflammatory and oxidative environment-related pathways, both in chronic and acute response. CONCLUSIONS: This research highlights the effects of external workload on elite athletes during a handball match and throughout the season. The study identifies deregulation in the immune system, mitochondrial functions, and various metabolic pathways during the match. Additionally, it establishes correlations between the external load and pathways associated with amino acids, inflammation, oxidative environment, and regulation. These findings offer insights into the immediate and chronic responses of athletes to physical effort.

Monitoring external load in elite male handball players depending on playing positions.

Monitoring workload is critical for elite training and competition, as well as preventing potential sports injuries. The assessment of external load in team sports has been provided with new technologies that help coaches to individualize training and optimize their team's playing system. In this study we characterized the physical demands of an elite handball team during an entire sports season. Novel data are reported for each playing position of this highly strenuous body-contact team sport. Sixteen world top players (5 wings, 2 centre backs, 6 backs, 3 line players) were equipped with a local positioning system (WIMU PRO) during fourteen official Spanish first league matches. Playing time, total distance covered at different running speeds, and acceleration variables were monitored. During a handball match, wings cover the greater distance by high-speed running (> 5.0 m·s-1): 410.3 ± 193.2 m, and by sprint (> 6.7 m·s-1): 98.0 ± 75.4 m. Centre backs perform the following playing position that supports the highest speed intensities during the matches: high-speed running: 243.2 ± 130.2 m; sprint: 62.0 ± 54.2 m. Centre backs also register the largest number of high-intensity decelerations (n = 142.7 ± 59.5) compared to wings (n = 112.9 ± 56.0), backs (n = 105.2 ± 49.2) and line players: 99.6 ± 28.9). This study provides helpful information for professional coaches and their technical staff to optimize training load and individualize the physical demands of their elite male handball players depending on each playing position.