[Expression in mouse L cells of mos-related ORAgp5 locus cloned from the human genome]. / Ekspressiia v L-kletkakh myshi mos-rodstvennogo lokusa ORAgp5, klonirovannogo iz genoma cheloveka.

The recombinant locus ORAgp5 containing the regions homologous to protooncogene mos was previously cloned from the human genome. In order to monitor the expression of this human genome region, we have transfected the recombinant phage gp5 into mouse LMtk cells. One of these transfectants contained several copies of gp5. ORAgp5 sequences were found to be expressed in 1.5 kb RNA from this clone.

[Tissue specificity of expression of k51 gene in rodents]. / Tkanevaia spetsifichnost' ékspressii kena k51 u gryzunov.

A new method for studding gene expression--the histoblotting was used for the detection of k51 gene expression in rodent tissues. We have demonstrated several advantages of this method over in situ hybridization for the study of gene expression in epitelial tissues in particular. The transcription of the new gene k51 was detected in the upper layers of mouse and rat skin during epidermis development before and after rat birth. These data were confirmed by Northern hybridization analysis. We found that the expression of k51 occurs mainly in flat multilayer epithelium and is not simply connected with the degree of days of rat embryo development; it is high in newborn skin and lowers during hair growth. The expression of k51 can serve as a marker of a certain stage of adult skin development.

[Structural characteristics and availability of DNA for solvent molecules in nuclei of Physarum polycephalum]. / Strukturnaia kharakteristika i dostupnost' DNK dlia molekul rastvoritelia v iadrakh Physarum polycephalum.

The rate constants of 1H--3H exchange between water and C8H-groups of purinic residues of DNA in solution and in nuclei of synchronous Physarum polycephalum at different phases of the cell cycle were measured. The nuclear membranes and nonhistone proteins were shown not to create additional hindrances and not to diminish the availability of DNA in nuclei for solvent molecules. A small increase of the rate of the 1H--3H exchange between water and the DNA of nuclei upon transition from the S-phase to the late G2-phase seems to reflect the process of chromatin decondensation connected with activation of the transcription and local changes of the secondary structure of DNA at the late G2-phase of the cell cycle.

[Regions of the human genome containing homologs of the oncogenes and retrovirus genes. 3. The primary structure of the mos-related region CL-1 from the ORAgp5 locus]. / Uchastki genoma cheloveka, soderzhashchie analogi onkogenov i genov retrovirusov. 3. Pervichnaia struktura mos-rodstvennogo uchastka CL-1 iz lokusa ORAgp5.

The nucleotide sequence of 686 bp from the cloned human genome locus gp5 has been determined. Analysis of this sequence has revealed a statistically significant homology with both the viral and murine mos genes. The region of mos homology contains two adjacent homologous domains, whereas their counterparts in viral mos gene are separated by 471 bp. The position of mos homologous region in the close vicinity to LTR of endogenous human viral-like repeat is in accordance with the hypothesis of retroviral involvement in the process of mos gene amplification.