RESUMO
The paper shows it possible to use stained polymeric microspheres, 1.7 microm in diameter, that contain viruses onto the surface, in the latex agglutination test to detect antibodies to the bovine serum viruses of infective rhinotracheitis, parainfluenza-3, viral diarrhea, respiratory syncytial infection, and adenoviral infection.
Assuntos
Infecções por Adenoviridae/veterinária , Anticorpos Antivirais/sangue , Doenças dos Bovinos/sangue , Rinotraqueíte Infecciosa Bovina/diagnóstico , Testes de Fixação do Látex/veterinária , Microesferas , Infecções por Respirovirus/veterinária , Viroses/veterinária , Vírus/metabolismo , Adenoviridae/imunologia , Adenoviridae/metabolismo , Infecções por Adenoviridae/sangue , Infecções por Adenoviridae/diagnóstico , Animais , Bovinos , Doenças dos Bovinos/diagnóstico , Herpesviridae/imunologia , Herpesviridae/metabolismo , Rinotraqueíte Infecciosa Bovina/sangue , Testes de Fixação do Látex/métodos , Vírus da Parainfluenza 3 Bovina/imunologia , Vírus da Parainfluenza 3 Bovina/metabolismo , Polímeros/metabolismo , Infecções por Respirovirus/sangue , Infecções por Respirovirus/diagnóstico , Suspensões/metabolismo , Viroses/sangue , Viroses/diagnóstico , Vírus/imunologiaRESUMO
The sbr gene of Drosophila melanogaster belongs to the NXF (nuclear export factor) family responsible for the mRNA transport from nucleus to cytoplasm. We have shown that in the heat-exposed (37 degrees C, 1 h) females, the l(1)ts403 (sbr10) mutation leads, in particular, to the high-frequency nondisjunction and loss of sex chromosomes in meiosis. For this trait, the incomplete dominance of the sbr10 mutation is observed. At the same time, the sbr10 mutation is recessive for many other traits of the heat-exposed flies: reduced viability, low fertility, impaired synthesis of the heat shock proteins, etc. The females heterozygous for the null allele (Df(1)vL4, a deletion eliminating gene srb) do not differ from females homozygous for the wild-type allele in frequency of the heat shock-induced nondisjunction and loss of sex chromosomes in meiosis. Because of this, the sbr10 mutation can be assigned to the gain-of-function alleles (those gaining the dominance function). Expression of the mutant sbr10 allele against the background of the wild-type allele suggests that in the heat shock-exposed females, the heat-modified product of this ts allele has a strong effect on sex chromosome disjunction in meiosis.
Assuntos
Drosophila melanogaster/genética , Meiose , Mutação , Não Disjunção Genética , Cromossomos Sexuais/genética , Animais , Proteínas de Drosophila/genética , Feminino , Fertilidade , Genes Dominantes , Resposta ao Choque Térmico/genética , Temperatura Alta , Perda de Heterozigosidade , Proteínas Nucleares/genética , Proteínas de Ligação a RNA/genéticaRESUMO
A DNA sequence from the 9F region of Drosophila melanogaster polytene chromosomes was cloned. Sequencing the cloned region and its comparison with the known sequences of the D. melanogaster genome showed that the cloned DNA part contains gene sbr and adjacent sequences. The literature data on the structure and functions of genes TAP in humans and Mex67 in yeast are discussed. These genes are orthologous to the sbr gene of Drosophila and control mRNA export from the nucleus to the cytoplasm. The literature evidence is consistent with the recessive expression of mutation l(1)ts403 (sbr10) upon heat treatment that is manifested as impaired HSP synthesis at the posttranscriptional level. However, it fails to explain the semidominant effect of the mutation manifested in high frequency of meiotic sex-chromosome nondisjunction in heat-treated females. A comparison of amino-acid sequences corresponding to the products of the three orthologous genes, TAP, Mex67, and sbr, showed that the sbr gene product of Drosophila is more similar to the human TAP factor than to the Mex67 factor in yeast.
