RESUMO
Observations on 277 children aged 3 months to 4 years with lesions of the nervous system, immunized with vaccine Act-HIB (Aventis Pasteur, France), were carried out in groups of children. The significant decrease of the level of Hib nasopharyngeal carrier state in 3.3 times and the decrease of total morbidity in acute infections of respiratory and ENT organs in 2.7 times occurred after immunization was revealed. The immunological effect of immunization on the level of protective titers against H. influenzae, type b, in children with organic CNS lesions in children was shown.
Assuntos
Anticorpos Antibacterianos/sangue , Portador Sadio/imunologia , Portador Sadio/prevenção & controle , Doenças do Sistema Nervoso Central/imunologia , Infecções por Haemophilus/imunologia , Infecções por Haemophilus/prevenção & controle , Vacinas Anti-Haemophilus/administração & dosagem , Haemophilus influenzae tipo b/imunologia , Polissacarídeos Bacterianos/administração & dosagem , Vacinação , Doença Aguda , Cápsulas Bacterianas , Doenças do Sistema Nervoso Central/complicações , Pré-Escolar , Infecções por Haemophilus/complicações , Haemophilus influenzae tipo b/isolamento & purificação , Humanos , Lactente , Injeções Intramusculares , Infecções Respiratórias/complicações , Infecções Respiratórias/imunologia , Infecções Respiratórias/prevenção & controle , SibériaRESUMO
Multiplex methylation-sensitive PCR was employed in studying the methylation of CpG islands in the RB1, p16/CDKN2A, p15/CDKN2B, p14/ARF, CDH1, MGMT, HIC1, and N33 promoter regions in breast carcinoma (105 tumors). Methylation was often observed for the two major suppressor genes involved in cell-cycle control through the Cdk-Rb-E2F signaling pathway, RB1 (18/105, 17%) and p16 (59/105, 56%); both genes were methylated in 13 tumors. Methylation involved p15 in two (2%) tumors; CDH1, in 83 (79%) tumors; MGMT, in eight (8%) tumors, and N33, in nine (9%) tumors. The p14 promoter was not methylated in the tumors examined.
Assuntos
Neoplasias da Mama/genética , Metilação de DNA , Genes Supressores de Tumor , Proteínas Supressoras de Tumor , Proteínas de Ciclo Celular/genética , Ilhas de CpG , Inibidor de Quinase Dependente de Ciclina p15 , Inibidor p16 de Quinase Dependente de Ciclina/genética , Feminino , Humanos , Fatores de Transcrição Kruppel-Like , O(6)-Metilguanina-DNA Metiltransferase/genética , Valores de Referência , Proteína do Retinoblastoma/genética , Fatores de Transcrição/genética , Proteína Supressora de Tumor p14ARF/genéticaRESUMO
Three experimental series, in which 20 test subjects took part, were carried out to investigate the effect of super-erythemic doses of ultraviolet irradiation on metabolic parameters. It was found that UV irradiation of 12-15% of the skin area led not only to skin changes but also to functional changes in certain organs and systems, which was indicated by variations of metabolic parameters.
Assuntos
Metabolismo/efeitos da radiação , Raios Ultravioleta , 11-Hidroxicorticosteroides/metabolismo , Colesterol/metabolismo , Diurese/efeitos da radiação , Contagem de Eritrócitos , Hematócrito , Hemoglobinas/análise , Humanos , Contagem de Leucócitos , Metabolismo dos Lipídeos , Proteínas/metabolismo , Doses de Radiação , Pele/efeitos da radiaçãoRESUMO
From the cell-free extract of fodder yeast Candida tropicalis NADP-specific glutamate dehydrogenase was isolated and partially purified (75-fold) by means of fractional precipitation by ammonium sulphate and ion-exchange chromatography on DEAE-cellulose. The preparation was investigated with the aid of polyacrylamide gel electrophoresis. Kinetic characteristics of the enzyme in the cell-free extract and partially purified preparation were derived.
