RESUMO
Leishmaniasis are neglected diseases transmitted by vectors that affect domestic and wild animals, including humans. Due to its incidence and lethality, this zoonosis is a worrying public health problem, making it essential to identify all links in the transmission chain. Infection of wild mammals by Leishmania spp. remains poorly understood, especially in southern Brazil. Therefore, the objective was to research, using the PCR technique, the presence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil. Carcasses of 96 animals were collected from highways in the Pelotas microregion, Rio Grande do Sul, southern Brazil and subjected to necropsies. Tissue fragments (spleen, skin, liver, kidney, heart, lung, lymph nodes, bone marrow and blood) were collected and genomic DNA was extracted. PCR protocols targeting the ITS1, kDNA and 18S genes were tested. We found no evidence of Leishmania spp. circulation in the studied population. However, epidemiological studies like this one are of great relevance, as they allow monitoring of the occurrence of pathogens and help identify possible risk areas. As these animals act as epidemiological markers for the presence of the microorganism, studies must be carried out continuously to understand whether there are sources of infection in the region.
Assuntos
Animais Selvagens , DNA de Protozoário , Leishmania , Mamíferos , Animais , Brasil/epidemiologia , Leishmania/isolamento & purificação , Leishmania/genética , DNA de Protozoário/análise , Animais Selvagens/parasitologia , Mamíferos/parasitologia , Reação em Cadeia da PolimeraseRESUMO
Abstract Lagochilascariasis is a parasitic disease caused by nematodes of the genus Lagochilascaris. These parasites occur in the neotropical region and their definitive hosts are wild animals, domestic dogs and felids and, accidentally, humans. Here, infection by Lagochilascaris minor is recorded for the first time in a wild felid, in Brazil.
Resumo A lagochilascaríase é uma doença parasitária causada por nematoides do gênero Lagochilascaris. Com distribuição neotropical, este parasito tem como hospedeiros definitivos animais silvestres, canídeos e felídeos domésticos e, acidentalmente, seres humanos. Registra-se a primeira ocorrência de Lagochilascaris minor em um felídeo silvestre no Brasil.
Assuntos
Animais , Masculino , Feminino , Ascaridoidea/isolamento & purificação , Panthera/parasitologia , Ascaridoidea/anatomia & histologia , Ascaridoidea/classificação , Brasil , Animais SelvagensRESUMO
Lagochilascariasis is a parasitic disease caused by nematodes of the genus Lagochilascaris. These parasites occur in the neotropical region and their definitive hosts are wild animals, domestic dogs and felids and, accidentally, humans. Here, infection by Lagochilascaris minor is recorded for the first time in a wild felid, in Brazil.
Assuntos
Ascaridoidea/isolamento & purificação , Panthera/parasitologia , Animais , Animais Selvagens , Ascaridoidea/anatomia & histologia , Ascaridoidea/classificação , Brasil , Feminino , MasculinoRESUMO
Abstract Parasitic diseases affecting wild carnivores remain largely unknown or poorly described. Dioctophymosis is a parasitosis caused by the nematode Dioctophyme renale that is found worldwide. It affects domestic and wild animals and has been reported frequently in Brazil. This paper reports dioctophymosis in a wild felid for the first time.
Resumo As doenças parasitárias que acometem os carnívoros silvestres ainda são desconhecidas ou pouco descritas. A dioctofimatose, parasitose de distribuição mundial causada pelo nematoide Dioctophyme renale, acomete animais domésticos e silvestres e tem sido relatada com frequência no Brasil. Este estudo reporta o primeiro caso de dioctofimatose em um felídeo silvestre.
Assuntos
Animais , Feminino , Dioctophymatoidea/isolamento & purificação , Panthera/parasitologia , Clima Tropical , Brasil , Rim/parasitologiaRESUMO
Parasitic diseases affecting wild carnivores remain largely unknown or poorly described. Dioctophymosis is a parasitosis caused by the nematode Dioctophyme renale that is found worldwide. It affects domestic and wild animals and has been reported frequently in Brazil. This paper reports dioctophymosis in a wild felid for the first time.
