[Campylobacteriosis in a low-income community in Buenos Aires, Argentina]. / Campilobacteriosis en una comunidad de bajos recursos de Buenos Aires, Argentina.

The authors describe the problem of campylobacteriosis in a low-income community located in southern Buenos Aires. Homes in the area were classified according to their total number of inhabitants, the number of children and animals living in the house and the amount of poultry meat consumed. Samples were taken from all the different types of homes that had been identified in order to isolate, identify and type Campylobacter sp., using the modified Skirrow technique, bio-chemical tests and the Lior technique respectively. The prevalences found were 16.96% in dogs, 20% in cats and 40% in poultry. The pathogens isolated were Campylobacter jejuni type II in 94.44% of the cases and Campylobacter upsaliensis in 5.55% of the cases. In poultry meat, 92.85% of the samples were positive (23.07% for type I and 76.92% for type II). The incidence of diarrhoea in children caused by C. jejuni was 0.4/1000 children/month (type I in all cases). A high prevalence of C. jejuni type II was detected in pets and in poultry meat. The source of infection in children may have been poultry meat.

Heterogeneidad entre tres cepas del virus de la fiebre aftosa, subtipo A24 Cruzeiro usadas para la producción de vacunas

Tres cepas del virus de la fiebre aftosa (FA), subtipo A24 Cruzeiro, usadas para la producción de vacuna, se compararon entre sí por mapeamiento T1 (fingerprinting) y por electroforesis en geles de poliacrilamida de las proteínas de la cápside. A pesar de resultar indistinguibles por ensayos de fijación de complemento, el mapeamiento T1 mostró variaciones genómicas entre ellas, que alcanzaron valores de hasta 4.0 por ciento. Estas diferencias se reflejaron en la movilidad electroforética de la principal proteína inmunogénica VP1. En una de las cepas, los padrones de migración de las proteínas estructurales VP2 y VP3 también fueron afectados. Los resultados ilustran la diversidad genómica potencial entre cepas usadas para la producción de vacunas contra la FA.

Three foot-and-outh disease (FMD) subtype A24 Cruzeiro virus strains used for the production of vaccines were compared with each other by T1, mapping (fingerprinting) and by polyacrylamide gel electrophoresis of the capsid proteins. Although indistinguishable by complement fixation tests, genomic variations were found among them by T1 mapping, reaching values of up to 4.0%. These differences were reflected in the electrophoretic mobility of the main immunogenic protein VP1. In one of the strains, migration patterns of structural proteins VP2 and VP3 were also affected. The results illustrate the potential genomic diversity of strains used for production of FMD vaccines.