RESUMO
Radicals induced by gamma-irradiation of sucrose, in the solid state at different temperatures and in aqueous solution, have been investigated by the spin trapping method. Electron spin resonance (ESR) combined with high performance liquid chromatography (HPLC), followed by spectral analysis with a simulation program (Voyons) revealed seven main radical species. A comparative study of the ESR signals from spin trapped gamma-induced radicals in some glycosides, disaccharides, 13C specifically labelled carbohydrates, as well as in several deoxysucroses and fructans, led to the assignment of a chemical structure to five out of the seven sucrose-nitroxide adducts previously evidenced. Sucrose is shown to be a conceivable model for the study of fructans gamma-radiolysis mechanism in aqueous solution.
Assuntos
Sacarose/efeitos da radiação , Sequência de Carboidratos , Espectroscopia de Ressonância de Spin Eletrônica , Radicais Livres/química , Raios gama , Dados de Sequência Molecular , Estrutura Molecular , Marcadores de Spin , Sacarose/químicaRESUMO
Radicals induced by gamma-irradiation in sucrose, in the solid state at different temperatures and in aqueous solution, have been studied by the spin-trapping method. Electron spin resonance (ESR) combined with high performance liquid chromatography (HPLC) then spectra analysis with a simulation program (VOYONS), revealed seven main radical species. Their nitrogen and hydrogen splitting constants were compared with those obtained from fructose and glucose units. Assignments of chemical structures are discussed for three radical species.
