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1.
Nat Commun ; 12(1): 2939, 2021 05 19.
Artigo em Inglês | MEDLINE | ID: mdl-34011960

RESUMO

Elucidation of non-canonical protein functions can identify novel tissue homeostasis pathways. Herein, we describe a role for the Bcl-2 family member BAD in postnatal mammary gland morphogenesis. In Bad3SA knock-in mice, where BAD cannot undergo phosphorylation at 3 key serine residues, pubertal gland development is delayed due to aberrant tubulogenesis of the ductal epithelium. Proteomic and RPPA analyses identify that BAD regulates focal adhesions and the mRNA translation repressor, 4E-BP1. These results suggest that BAD modulates localized translation that drives focal adhesion maturation and cell motility. Consistent with this, cells within Bad3SA organoids contain unstable protrusions with decreased compartmentalized mRNA translation and focal adhesions, and exhibit reduced cell migration and tubulogenesis. Critically, protrusion stability is rescued by 4E-BP1 depletion. Together our results confirm an unexpected role of BAD in controlling localized translation and cell migration during mammary gland development.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Proteínas de Ciclo Celular/metabolismo , Glândulas Mamárias Animais/crescimento & desenvolvimento , Glândulas Mamárias Animais/metabolismo , Glândulas Mamárias Humanas/crescimento & desenvolvimento , Glândulas Mamárias Humanas/metabolismo , Proteína de Morte Celular Associada a bcl/metabolismo , Substituição de Aminoácidos , Animais , Linhagem Celular , Movimento Celular/genética , Feminino , Técnicas de Introdução de Genes , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Modelos Animais , Morfogênese , Proteínas Mutantes/química , Proteínas Mutantes/genética , Proteínas Mutantes/metabolismo , Organoides/crescimento & desenvolvimento , Organoides/metabolismo , Fosforilação , Biossíntese de Proteínas , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Serina/química , Proteína de Morte Celular Associada a bcl/deficiência , Proteína de Morte Celular Associada a bcl/genética
2.
Chromosoma ; 123(4): 373-83, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24676866

RESUMO

Sex chromosomes differ from autosomes by dissimilar gene content and, at a more advanced stage of their evolution, also in structure and size. This is driven by the divergence of the Y or W from their counterparts, X and Z, due to reduced recombination and the resulting degeneration as well as the accumulation of sex-specific and sexually antagonistic genes. A paradigmatic example for Y-chromosome evolution is found in guppies. In these fishes, conflicting data exist for a morphological and molecular differentiation of sex chromosomes. Using molecular probes and the previously established linkage map, we performed a cytogenetic analysis of sex chromosomes. We show that the Y chromosome has a very large pseudoautosomal region, which is followed by a heterochromatin block (HCY) separating the subtelomeric male-specific region from the rest of the chromosome. Interestingly, the size of the HCY is highly variable between individuals from different population. The largest HCY was found in one population of Poecilia wingei, making the Y almost double the size of the X and the largest chromosome of the complement. Comparative analysis revealed that the Y chromosomes of different guppy species are homologous and share the same structure and organization. The observed size differences are explained by an expansion of the HCY, which is due to increased amounts of repetitive DNA. In one population, we observed also a polymorphism of the X chromosome. We suggest that sex chromosome-linked color patterns and other sexually selected genes are important for maintaining the observed structural polymorphism of sex chromosomes.


Assuntos
Poecilia/genética , Polimorfismo Genético , Cromossomo X/genética , Cromossomo Y/genética , Animais , Bandeamento Cromossômico , Impressões Digitais de DNA , Feminino , Indóis/metabolismo , Cariotipagem , Masculino , Meiose/genética , Metáfase/genética
3.
Proc Biol Sci ; 276(1665): 2195-208, 2009 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-19324769

RESUMO

We report construction of a genetic linkage map of the guppy genome using 790 single nucleotide polymorphism markers, integrated from six mapping crosses. The markers define 23 linkage groups (LGs), corresponding to the known haploid number of guppy chromosomes. The map, which spans a genetic length of 899 cM, includes 276 markers linked to expressed genes (expressed sequence tag), which have been used to derive broad syntenic relationships of guppy LGs with medaka chromosomes. This combined linkage map should facilitate the advancement of genetic studies for a wide variety of complex adaptive phenotypes relevant to natural and sexual selection in this species. We have used the linkage data to predict quantitative trait loci for a set of variable male traits including size and colour pattern. Contributing loci map to the sex LG for many of these traits.


Assuntos
Tamanho Corporal/genética , Pigmentos Biológicos/genética , Poecilia/anatomia & histologia , Poecilia/fisiologia , Locos de Características Quantitativas/genética , Animais , Mapeamento Cromossômico , Feminino , Ligação Genética , Masculino , Dados de Sequência Molecular , Poecilia/genética , Sintenia
4.
Genetics ; 182(1): 365-74, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19299341

RESUMO

Among different teleost fish species, diverse sex-determining mechanisms exist, including environmental and genetic sex determination, yet chromosomal sex determination with male heterogamety (XY) prevails. Different pairs of autosomes have evolved as sex chromosomes among species in the same genus without evidence for a master sex-determining locus being identical. Models for evolution of Y chromosomes predict that male-advantageous genes become linked to a sex-determining locus and suppressed recombination ensures their co-inheritance. In the guppy, Poecilia reticulata, a set of genes responsible for adult male ornaments are linked to the sex-determining locus on the incipient Y chromosome. We have identified >60 sex-linked molecular markers to generate a detailed map for the sex linkage group of the guppy and compared it with the syntenic autosome 12 of medaka. We mapped the sex-determining locus to the distal end of the sex chromosome. We report a sex-biased distribution of recombination events in female and male meiosis on sex chromosomes. In one mapping cross, we observed sex ratio and male phenotype deviations and propose an atypical mode of genetic sex inheritance as its basis.


Assuntos
Mapeamento Cromossômico , Ligação Genética , Poecilia/genética , Cromossomos Sexuais , Cromossomo Y/genética , Animais , Cruzamentos Genéticos , Marcadores Genéticos , Fenótipo , Recombinação Genética
5.
Zebrafish ; 5(4): 265-78, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19133825

RESUMO

Male ornamental traits of the guppy, Poecilia reticulata, provide an outstanding example of natural variation in sex-linked male-advantageous traits that are shaped by both sexual and environmental selection. A substantial fraction of the underlying genes is known to be genetically linked to the sex-determining region on the differentiating Y-chromosome. Intercrosses between parental populations originating from geographically distant locations in East Trinidad and Cumaná (Venezuela) were used to study segregation of ornamental traits in male progeny. In addition, we performed backcrosses to compare segregation of ornaments in presence or absence of prominent traits linked to the Y-chromosome. Another backcross strategy involving XY females from the laboratory strain zebrinus maculatus allowed studying additive and dominant effects of alleles on two different Y-chromosomes on pattern formation. For genetic mapping, we have previously developed nuclear SNP markers linked to expressed genes, including several genes known to be important for pattern formation in other species. Of these candidate genes 15 were placed on 11 different linkage groups. Our phenotypic and genotypic analysis of progeny from mapping crosses and backcrosses suggests several genetic mechanisms that enhance natural variation, namely, additive effects of codominant alleles, suppressive actions of dominant alleles, and a complex interplay between sex-linked and autosomal cofactors.


Assuntos
Poecilia/genética , Poecilia/fisiologia , Caracteres Sexuais , Pigmentação da Pele/genética , Pigmentação da Pele/fisiologia , Animais , Feminino , Genes Ligados ao Cromossomo Y , Genótipo , Masculino , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas
6.
BMC Genomics ; 8: 269, 2007 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-17686157

RESUMO

BACKGROUND: The guppy, Poecilia reticulata, is a well-known model organism for studying inheritance and variation of male ornamental traits as well as adaptation to different river habitats. However, genomic resources for studying this important model were not previously widely available. RESULTS: With the aim of generating molecular markers for genetic mapping of the guppy, cDNA libraries were constructed from embryos and different adult organs to generate expressed sequence tags (ESTs). About 18,000 ESTs were annotated according to BLASTN and BLASTX results and the sequence information from the 3' UTRs was exploited to generate PCR primers for re-sequencing of genomic DNA from different wild type strains. By comparison of EST-linked genomic sequences from at least four different ecotypes, about 1,700 polymorphisms were identified, representing about 400 distinct genes. Two interconnected MySQL databases were built to organize the ESTs and markers, respectively. A robust phylogeny of the guppy was reconstructed, based on 10 different nuclear genes. CONCLUSION: Our EST and marker databases provide useful tools for genetic mapping and phylogenetic studies of the guppy.


Assuntos
Etiquetas de Sequências Expressas , Filogenia , Poecilia/genética , Polimorfismo de Nucleotídeo Único , Animais , Sequência de Bases , Primers do DNA , DNA Complementar , Bases de Dados Genéticas , Poecilia/classificação , Cromossomos Sexuais
7.
Proc Biol Sci ; 274(1606): 33-42, 2007 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-17015333

RESUMO

Identification of genes that control variation in adaptive characters is a prerequisite for understanding the processes that drive sexual and natural selection. Male coloration and female colour perception play important roles in mate choice in the guppy (Poecilia reticulata), a model organism for studies of natural and sexual selection. We examined a potential source for the known variation in colour perception, by analysing genomic and complementary DNA sequences of genes that code for visual pigment proteins. We find high sequence variability, both within and between populations, and expanded copy number for long-wave sensitive (LWS) opsin genes. Alleles with non-synonymous changes that suggest dissimilar spectral tuning properties occur in the same population and even in the same individual, and the high frequency of non-synonymous substitutions argues for diversifying selection acting on these proteins. Therefore, variability in tuning amino acids is partitioned within individuals and populations of the guppy, in contrast to variability for LWS at higher taxonomic levels in cichlids, a second model system for differentiation owing to sexual selection. Since opsin variability parallels the extreme male colour polymorphism within guppy populations, we suggest that mate choice has been a major factor driving the coevolution of opsins and male ornaments in this species.


Assuntos
Duplicação Gênica , Modelos Genéticos , Poecilia/genética , Opsinas de Bastonetes/genética , Alelos , Sequência de Aminoácidos , Animais , Clonagem Molecular , Percepção de Cores/genética , Evolução Molecular , Dosagem de Genes , Haplótipos , Dados de Sequência Molecular , Filogenia , Polimorfismo Genético , Opsinas de Bastonetes/química , Opsinas de Bastonetes/fisiologia , Seleção Genética , Alinhamento de Sequência
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