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Proteins ; 71(4): 1843-52, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18175312

RESUMO

Sulfolobus solfataricus carboxypeptidase (CPSso) is a thermostable zinc-metalloenzyme, consisting of four identical subunits with a M(r) of 43,000. In a previous paper (Occhipinti et al., Biophys J 2003; 85:1165-1175), we developed a structure of the enzyme by molecular modeling and validated it by site-directed mutagenesis and small angle X-ray scattering. Here, we report investigations aimed at further validating the model, as well as at identifying molecular determinants responsible for thermostability. To this end, we took advantage of mass spectrometry techniques, notably LC-MS/MS. The structure was confirmed by such approaches, in that they lead to the identification of a disulfide bridge formed by Cys286 and Cys293, whose location in the model is well suited for giving rise to the crosslink. More notably, we also identified a protease-resistant core consisting of the N- and C-terminal antiparallel alpha-helices, which in the model are predicted to interact with each other via hydrophobic quadrants. On the basis of the model, we also tentatively identified the most tightly interacting residues as Leu7, Ala380, and Leu376. Although the replacement of Ala380 by serine did not detectably impair protein stability, a dramatic drop in thermostability was observed when the two leucines were replaced by either aspartate (L7D; L376D) or asparagine (L7N; L376N). We then investigated the kinetic thermal stability of the wild type and the mutants by determining the thermodynamic activation parameters, DeltaG++, DeltaH++, and DeltaS++. Besides highlighting the key role of the hydrophobic core in thermostability, these results suggest clearly different mechanisms of destabilization by the single mutations, depending on whether the leucines are replaced by asparagines or aspartates.


Assuntos
Carboxipeptidases , Temperatura Alta , Espectrometria de Massas , Modelos Moleculares , Mutagênese Sítio-Dirigida , Sulfolobus solfataricus/enzimologia , Alquilação , Sequência de Aminoácidos , Substituição de Aminoácidos , Asparagina/metabolismo , Ácido Aspártico/metabolismo , Carboxipeptidases/análise , Carboxipeptidases/química , Carboxipeptidases/genética , Cisteína/química , Dissulfetos/química , Ativação Enzimática/efeitos dos fármacos , Estabilidade Enzimática , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Cinética , Modelos Químicos , Dados de Sequência Molecular , Peso Molecular , Oxirredução , Pepsina A/farmacologia , Engenharia de Proteínas/métodos , Estrutura Secundária de Proteína , Subunidades Proteicas/química , Serina/metabolismo , Termodinâmica , Tripsina/farmacologia
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