RESUMO
AIM: To study the effect of sex- and age-related features, social aspects and progression of alcoholism on tobacco smoking (TS), tobacco dependence (TD) and alcohol dependence (AD) in the Chuvash Republic. MATERIAL AND METHODS: Authors questioned 769 inpatients with AD and then performed detailed examination and questioning of 390 patients. RESULTS AND CONCLUSION: 74.4% patients with AD (82.2% men and 41.6% women) were cigarette smokers. According to the Fagerström's test, 79.2% had Nicotine Dependence (ND), 80.3% smoked more than 10 cigarettes per day. ND was diagnosed much more often in men, TS in them was more intense in comparison with women. There were highly significant correlations between alcoholism progression and TS or TD. Patients with AD had more significant ND compared to total population. They started TS much earlier, more often didn't prefer a particular brand of cigarettes, smoked high pitch cigarettes. Rejuvenation of TS onset with the fast formation of ND and AD is noted.
Assuntos
Neoplasias Ovarianas , Fatores Etários , Idoso , Animais , Modelos Animais de Doenças , Feminino , Humanos , Inflamação/patologia , Camundongos , Pessoa de Meia-Idade , Mutação , Transplante de Neoplasias , Neoplasias Experimentais , Neoplasias Ovarianas/etiologia , Neoplasias Ovarianas/genética , Neoplasias Ovarianas/patologia , Neoplasias Ovarianas/fisiopatologia , Ovário/patologia , Ovário/fisiopatologia , Fenótipo , Ratos , Fatores de Risco , Viroses/complicaçõesRESUMO
The pregrowth hormone DNA was synthesized by polymerase chain reaction from a pituitary gland cDNA library of Baikal omul (Coregonus autumnalis Pallas). A cDNA was sequenced and found to encode a precursor protein of 210 amino acid residues, which included a putative signal peptide of 22 amino acid residues. Sequence comparison reveals close similarity of the omul growth hormone to those of other Salmoniformes species.
Assuntos
DNA/química , Peixes/genética , Hormônio do Crescimento/genética , RNA Mensageiro/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Dados de Sequência Molecular , Hipófise/metabolismo , Salmoniformes/genética , Homologia de Sequência de AminoácidosRESUMO
Two types of cDNA encoding gonadotropin beta subunits (GTH beta) were isolated from a cDNA library prepared from pituitary gland of Baikal omul (Coregonus autumnalis migratorius Georgi). The nucleotide sequences of cDNA were determined. The CTHI beta and GTHII beta cDNAs code for polypeptides of 137 and 142 amino acids, respectively. Both of them include a putative signal peptide of 24 amino acids. The predicted amino acid structures of omul gonadotropins were compared with those of other vertebrate species.
Assuntos
Peixes/genética , Gonadotropinas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , DNA Complementar , Gonadotropinas/química , Dados de Sequência Molecular , Homologia de Sequência de AminoácidosRESUMO
We constructed a complementary DNA (cDNA) library from Baikal omul (Coregonus autumnalis migratorius Georgi) pituitary glands. Synthetic oligonucleotide probes corresponding to chum salmon prolactin (PRL) cDNA were used to select the recombinant plasmids carrying the omul PRL gene. The larger insert was sequenced and found to encode a polypeptide of 210 amino acid residues, including a putative signal sequence of 23 amino acids. Nucleotide and predicted amino acid sequence of omul PRL showed high homology with other salmonid PRLs.
