RESUMO
We studied blood serum levels of neurosteroids, dehydroepiandrosterone and its sulfate, in individuals with personality disorders convicted of serious violent crimes. The data were compared with that of a group of mentally and physically healthy persons convicted of acquisitive crimes, and with that of the control group. Significant increase in DHEA in both groups of convicts in comparison with the control was shown. The level of dehydroepiandrosterone sulfate remained unchanged. Increased dehydroepiandrosterone level in the convicted individuals with personality disorders is probably more associated with detention stress than directly with psychopathology or criminal aggression.
Assuntos
Agressão/psicologia , Criminosos/psicologia , Sulfato de Desidroepiandrosterona/sangue , Desidroepiandrosterona/sangue , Transtornos da Personalidade/sangue , Adulto , Crime/psicologia , Humanos , Masculino , Pessoa de Meia-Idade , Estresse Psicológico/sangue , Violência/psicologia , Adulto JovemRESUMO
The present paper deals with the study of the efficiency of oral use of the antioxidative drug Immugen (a complex of alpha-tocopherol, oubichinone, selenium aspartate, methionine, and soyabean phospholipids) on a rabbit model of severe alkaline-induced corneal burn. The investigations have indicated that addition of Immugen to the rabbit feed exerts a significant positive effect on the parameters of the local antioxidative system of the eye and causes an increase in the activity of superoxide dismutase and catalase, and, on day 14, in antioxidative activity. The early experimental periods were marked by a slight rise in the frequency of deep corneal ulcerations. Moreover, the long-term clinical effect of use of Immugen appears as a significant increase in the area of the transparency-preserving affected cornea. The findings suggest that the antioxidants can show their optimal effect in the complex therapy for burn processes, including the use of proteinase inhibitors.
Assuntos
Antioxidantes , Queimaduras Químicas/metabolismo , Lesões da Córnea , Queimaduras Oculares/metabolismo , Lágrimas/metabolismo , Álcalis/toxicidade , Animais , Antioxidantes/metabolismo , Antioxidantes/uso terapêutico , Queimaduras Químicas/tratamento farmacológico , Queimaduras Químicas/patologia , Catalase/metabolismo , Córnea/efeitos dos fármacos , Córnea/patologia , Neovascularização da Córnea/metabolismo , Neovascularização da Córnea/patologia , Neovascularização da Córnea/prevenção & controle , Modelos Animais de Doenças , Queimaduras Oculares/induzido quimicamente , Queimaduras Oculares/tratamento farmacológico , Seguimentos , Coelhos , Superóxido Dismutase/metabolismo , Resultado do TratamentoRESUMO
Five complementing recessive mutations that exhibit increased bacterial transposon Tn5 precise excision in yeast Saccharomyces cerevisiae were obtained by ethylmethanesulfonate treatment. One of these mutations (tex1) was submitted to extensive genetic analysis. tex1 is a recessive temperature-sensitive mutation resulting in a 20-100-fold increase in Tn5 excision. It also has increased frequencies of ochre mutation reversion, of forward mutation to canavanine resistance, and loss of chromosome III or its right arm. The possible mechanism of tex1 effects is discussed.
Assuntos
Elementos de DNA Transponíveis , Mutagênese Insercional , Saccharomyces cerevisiae/genética , Alelos , Plasmídeos , Mapeamento por Restrição , Transformação GenéticaRESUMO
The collection of overlapping lys2 deletions (five in the chromosomal and seven in the plasmid LYS2 gene) is constructed in this work. The deletions overlap the whole coding region of the gene and provide the system for intragenic recombinational mapping of lys2 mutations in one of 14 controlled regions. A portion of these regions can be correlated with the regions on the physical map of LYS2. Mutations in two regions can be easily cloned. The system constructed gives the possibility for the study of intragenic and molecular specificity of mutagenesis.
Assuntos
Genes Fúngicos , Mutação , Saccharomyces cerevisiae/genética , Alelos , Deleção Cromossômica , Cromossomos Fúngicos , Engenharia Genética , Plasmídeos , Mapeamento por RestriçãoAssuntos
Hérnia/mortalidade , Adulto , Fatores Etários , Idoso , Feminino , Hérnia/complicações , Hérnia Femoral/mortalidade , Hérnia Inguinal/mortalidade , Hérnia Umbilical/mortalidade , Hospitalização , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/mortalidade , Federação Russa , Fatores de TempoRESUMO
We have demonstrated that precise excision of bacterial transposon Tn5 can occur in the yeast, Saccharomyces cerevisiae. Tn5 insertions in the yeast gene LYS2 were generated by transposon mutagenesis made in Escherichia coli by means of a lambda::Tn5 vector. Nine insertions of Tn5 into the structural part of the yeast LYS2 gene situated in a shuttle episomal plasmid were selected. All the plasmids with a Tn5 insertion were used to transform yeast strains carrying a deletion of the entire LYS2 gene or a deletion of the part of LYS2 overlapping the point of insertion. All insertions inactivated the LYS2 gene and were able to revert with low (about 10(-8) frequencies to lysine prototrophy. Restriction analysis of revertant plasmids revealed them to be indistinguishable from the original plasmid without Tn5 insertion. DNA sequencing of the regions containing the points of insertions, made for two revertants, proved that Tn5 excision was completely precise.
