A pilot study on active and passive ex vivo characterisation of the urinary bladder and its impact on three-dimensional modelling.

Insight into the global deformation of the urinary bladder during passive and active phases is crucial for understanding the biomechanics and function of the organ. Therefore, in the present study, the three-dimensional deformations of the porcine urinary bladder were investigated using 10 cameras in ex vivo experiments. Voltages between 20 V and 40 V were applied to induce contraction without outflow (isovolumetric) and against different back pressures (isobaric). The fluid volume in the bladder and the fluid volume pushed out of the bladder in the active state were measured. During filling, a roughly constant pressure of 2.5-4 cmH2O was measured for a large volume range, followed by a steep increase. Overall, the urinary bladder shape changes from elliptical to spherical in the active phase, resulting in a more homogeneous stress field. The active pressure decreases with increasing volume, while the actively generated stress increases up to 65 kPa at the maximum volume examined. Smaller filling volumes and lower back pressures allowed complete emptying, whereas higher back pressures prevent full emptying from larger filling states. Finally, a recently developed three-dimensional model was used to describe the active and passive bladder characteristics in order to qualitatively represent the mechanical properties. Overall, this study provides for the first time a comprehensive experimental data set at organ level that leads to an improved understanding of load transfer mechanisms within the urinary bladder and serves to validate corresponding models.

Predicting muscle tissue response from calibrated component models and histology-based finite element models.

Skeletal muscle is an anisotropic soft biological tissue composed of muscle fibres embedded in a structurally complex, hierarchically organised extracellular matrix. In a recent work (Kuravi et al., 2021) we have developed 3D finite element models from series of histological sections. Moreover, based on decellularisation of fresh tissue samples, a novel set of experimental data on the direction dependent mechanical properties of collagenous ECM was established (Kohn et al., 2021). Together with existing information on the material properties of single muscle fibres, the combination of these techniques allows computing predictions of the composite tissue response. To this end, an inverse finite element procedure is proposed in the present work to calibrate a constitutive model of the extracellular matrix, and supplementary biaxial tensile tests on fresh and decellularised tissues are performed for model validation. The results of this rigorously predictive and thus unforgiving strategy suggest that the prediction of the tissue response from the individual characteristics of muscle cells and decellularised tissue is only possible within clear limits. While orders of magnitude are well matched, and the qualitative behaviour in a wide range of load cases is largely captured, the existing deviations point at potentially missing components of the model and highlight the incomplete experimental information in bottom-up multiscale approaches to model skeletal muscle tissue.

Location- and layer-dependent biomechanical and microstructural characterisation of the porcine urinary bladder wall.

The knowledge of the mechanical properties of the urinary bladder wall helps to explain its storage and micturition functions in health and disease studies; however, these properties largely remain unknown, especially with regard to its layer-specific characteristics and microstructure. Consequently, this study entails the assessment of the layer-specific differences in the mechanical properties and microstructure of the bladder wall, especially during loading. Accordingly, ninety-two (n=92) samples of porcine urinary bladder walls were mechanically and histologically analysed. Generally, the bladder wall and different tissue layers exhibit a non-linear stress-stretch relationship. In this study, the load transfer mechanisms were not only associated with the wavy structure of muscular and mucosal layers, but also with the entire bladder wall microstructure. Contextually, an interplay between the mucosal and muscular layers could be identified. Therefore, depending on the region and direction, the mucosal layer exhibited a stiffer mechanical response to equi-biaxial loading than that offered by the muscular layer when deformed to stretch levels higher than λ=1.6 to λ=2.2. For smaller stretches, the mucosal layer evinces no significant mechanical reaction, while the muscular layer bears the load. Owing to the orientation of its muscle fibres, the muscular layer shows an increased degree of anisotropy compared to the mucosal layer. Furthermore, the general incompressibility assumption is analysed for different layers by measuring the change in thickness during loading, which indicated a small volume loss.