[The immunological activity of Neisseria meningitidis lipo-oligosaccharide incorporated into liposomes]. / Immunologicheskaia aktivnost' lipooligosakharida Neisseria meningitidis, vkliuchennogo v liposomy.

Immune response to lipooligosaccharide (LOS), isolated from group B N. meningitidis cell wall, was studied after its incorporation into liposomes. The adjuvant effect produced by liposomal LOS, more pronounced in comparison with that produced by native LOS, was observed after the injection of the preparation by different routes (intravenous, intraperitoneal and subcutaneous injections), but the highest level of response was achieved after the intravenous and intraperitoneal injections of the preparation. Experiments aimed at the study of the dynamics of plaque-forming cells and the level of antibodies after intraperitoneal immunization of CBA mice with LOS revealed that immune response to liposomal LOS was more intense and prolonged than that to the free antigen. Liposomal LOS induced genetically nonrestricted and T-independent immune response. This fact was confirmed in experiments on mice having different haplotypes, such as CBA (k), C57BL/6 (b), BALB/c (d) and in nude mice with congenital thymic aplasia. In addition, liposomal LOS induced mainly the accumulation of IgM and IgG and did not produce the effect of immunological memory.

[Possibility of the unification of directed drug transport as illustrated by liposome transport to target antigens]. / Vozmozhnost' unifikatsii napravlennogo transporta lekarstv na primere transporta liposom k naboru antigenov misheni.

A new approach to the targeted drug delivery is described. Unlike previous methods, associated with the necessity of specific immunoglobulin immobilization on the surface of drug-containing microcontainer, the present approach permits targeted transport of standardized container to a set of target antigens, using intermediate molecules-mediators possessing high and specific affinity to both vector antibody and standardized container. It was shown that simultaneous targeting of 14C-labeled liposomes to three target antigens using avidin-biotin system permits the increase in liposome binding to target monolayer by 30-50%, as compared to targeting of the same amount of liposomes to one antigen. The method developed is particularly promising in cases when relative availability of target antigens in the target organ is unknown.

[Glyceraldehyde-3-phosphate dehydrogenase stabilization by the introduction of intersubunit linkages]. / Stabilizatsiia glitseral'degid-3-fosfatdegidrogenazy putem vvedeniiia mezhsub"edinichnykh sshivok.

To work out methods of synthesis of stabilized enzymatic preparations for clinical application, subunit enzyme glyceraldehyde-3-phosphatedehydrogenase was treated with bifunctional reagents--aliphatic diacids, activated by water-soluble carbodiimide. Gel-electrophoresis in the presence of natrium dodecyl sulfate showed the presence of a linkage between the subunits. The maximal thermostability was observed in the preparations treated with amber and glutaric acid. These preparations also contained the maximal amount of the enzymatic fraction, which did not dissociate into subunits. The data of EPR-spectroscopy showed that the preparations of the enzyme with the introduced intersubunit linkages had a lowered molecular mobility.