[TOTRP EXPERIENCE IN TREATING WOMEN'S STRESS CONTINUITY IN WOMEN].

The aim of the study was to evaluate the efficacy and safety of the TOTRP (tension-free vaginal tape-obturator retropublic) methodology, as well as the frequency of de novo urgency symptoms. A retrospective study included 32 women with stressful and mixed urinary incontinence with a predominance of the stress component without concomitant genital prolapse, as well as without surgical intervention for a history of urinary incontinence and pelvic prolapse. Patients underwent surgical intervention according to the TOTRP method using Lintex UroSling® polypropylene tape from February to October 2018. According to the results of the ICIQ-SF and OAB awareness Tool questionnaires, 14 (43.7%) patients were registered prior to surgical treatment. with an average degree of stress urinary incontinence, 18 (56.3%) - severe; 19 (59%) patients with mild bladder hyperactivity, 4 (12.5%) - moderate. Through surgical intervention, stress urinary incontinence was completely stopped in 26 (81.25%) and transferred to mild degree in 2 (6.25%), in 5 (15.6%) - moderate degree. So, the effectiveness of the TOTRP technique was 87.5%. Postoperative urgency was 8 (25%), of which de novo - 5 (15.63%). The treatment was characterized by the complete absence of intraoperative complications.

[NON-RESECTOSCOPIC ABLATION OF ENDOMETRY IN BENIGN UTERINE DISEASES (REVIEW)].

Objective - to conduct a systematic analysis of the data available in the modern literature on non-rectoscopic endometrial ablation (NRAE). MATERIALS AND METHODS: The review includes data from world studies for the last 7 years. RESULTS: second generation systems based on different surgical energies and parameters of their performance. Presents the results of evaluating the effectiveness of devices and patient satisfaction; studies of possible complications, frequency of repeated operations, contraception. CONCLUSION: Analysis of the literature data showed that the devices have high efficiency indicators, in particular, up to 97.1%, undesirable consequences are rare. NRAE in case it is an alternative to hysterectomy. It is necessary to introduce NRAE into practical health care as a method capable of providing organ-preserving treatment to a woman.

[Ways of apoptosis development in human lymphocytes, induced by UV-irradiation].

The level of DNA damage and cytochrome c content in human lymphocytes in the dynamics of apoptosis induced by UV-light (240-390 nm) at doses of 151, 1510 and 3020 J/m2 is studied. DNA fragmentation is revealed in 20 h after UV-irradiation of lymphocytes at doses mentioned above. It is shown that DNA damages (single strand breaks) appear immediately after UV-irradiation of lymphocytes at doses of 1510 and 3020 J/m2 (comets of C1 type) and reach their maximum 6 h after cell modification (comets of C2 and C3 types). It is concluded that p53-dependent and receptor caspase pathways are involved in apoptosis development in the human lymphocytes, modified after UV-irradiation.

[Ways of realizing apoptosis of human lymphocytes induced by UV-light and reactive oxygen species].

Changes of DNA structural condition, the level of membrane Fas-receptor expression, caspase-3 functional activity, concentrations of Ca2+, p53 and cytochrome c proteins of human lymphocytes in dynamics of apoptosis development induced by UV-light (240-390 nm) at doses 151, 1510, 3020 J/m2 and reactive oxygen species (superoxide anion-radical, hydroxyl radicals, hydrogen peroxide, singlet oxygen) have been studied. UV-light and reactive oxygen species have been established to induce fragmentation of lymphocyte DNA after 20 h incubation of the modified cells. It has been shown, that the increase in the expression level of membrane death Fas-receptors is observed during 1-5 h after exposure oflymphocytes to UV-light and ROS compared with intact cells. Also revealed is augmentation of lymphocyte caspase-3 functional activity 4 h after generation of singlet oxygen, hydroxyl radical and hydrogen peroxide addition, as well as 8 and 24 and 6 and 8 h after UV-irradiation of the cells at doses 151 and 1510 J/m2, correspondingly. Using DNA-comet method made it possible to tape that DNA damages (single-strand breaks) appear 15-20 min after lymphocyte UV-irradiation at doses 1510 and 3020 J/m and addition of hydrogen peroxide in concentration 10(-6) mol/l (C1 type comet) and reach their maximum 6 h after modification of the cells (C2 and C3 type comets). It has been observed, that 6 h after exposure oflymphocytes to hydrogen peroxide and UV-light at doses 1510 and 3020 J/m2, the p53 level of investigated cells raises. It has also been shown that the higher level of calcium in lymphocyte cytosol in conditions of UV-light exposure (1510 J/m2) and exogenous generation of reactive oxygen species is caused by Ca2+ exit from intracellular depots as a result of activating the components of the phosphoinositide mechanism for transferring information into a cell. Ideas about correlation between alterations of the calcium level and initiation of programmed cellular destruction of human lymphocytes after exposure to UV-irradiation and ROS is proposed. The authors come to the conclusion about the leading role of receptor-mediated (Fas-dependent) caspase- and p53-dependent ways of realizing apoptosis oflymphocytes induced by UV-light at doses 151 and 1510 J/m2 and active oxygen metabolites. The pattern of the possible intracellular events leading to apoptotic destruction of lymphocytes after their UV-irradiation is offered.

[Fragmentation of DNA lymphocytes the human in dynamics of development apoptosis, induced influence of UV-radiation and reactive oxygen species].

It is established that UV-light (240-390 nm) in doses of 151, 1510 and 3020 J/m2 and reactive oxygen species and singlet oxygen induce DNA fragmentation lymphocytes cells of the human 20 h after influence. Using a method of DNA-comets it is revealed that DNA damages (single strand breaks) are found out right after UV-irradiations of lymphocytes in doses of 1510 and 3020 J/m2 and additions hydrogen peroxide in concentration of 10-6 mol/l (a comet of type C1) and reach a maximum through 6 h after influence on of cells UV-light and ROS (comets of types C2 and C3). Assumption about the leading part of a p53-dependent way in realization apoptosis human lymphocytes in the conditions of influence of UV-light and reactive oxygen species is put forward.

Second messengers cAMP, Ca2+, and NO modulate functional properties of human lymphocytes under conditions of exposure to UV light.

We studied the effect of UV light (240-390 nm) in doses of 75.5-4530 J/m(2) on functional properties and level of intracellular messengers (cAMP, Ca(2+), NO) in lymphocytic cells of the peripheral blood. Correcting effects of UV light in a dose of 151 J/m(2) on cytotoxic activity of lymphocytes against Ehrlich ascetic carcinoma and calcium concentration in lymphocyte cytosol were observed. We found that UV light reduces the cAMP content and increases NO concentration (151-4530 J/m(2)) in photomodified cells. The involvement of adenylate cyclase and phosphoinositide signaling pathway into the mechanisms of modulation of functional properties of lymphocytes after exposure to UV light was demonstrated.

[Receptor caspase-dependent and caspase-independent ways of human lymphocytes apoptosis induced by UV-radiation].

Changes of a structural condition of DNA, expression level of the membrane Fas-receptor and of functional activity of the human lymphocytes caspase-3 in dynamics of apoptosis development, induced by influence of UV-light (240-390 nm) in doses 151, 1510 and 3020 J/m2 are studied. It is established, that the UV-irradiation of lymphocytes in doses of 151 and 1510 J/m2 causes realisation of receptor caspase-dependent apoptosis way. The assumption about switching possibility of receptor caspase-dependent way apoptosis on receptor caspase-independent way at increase of a dose of UV-light to 3020 J/m2 is made.

[Structurally functional modifications of the human lymphocytes induced by influence of UV-light].

The influence of UV-light (240-390 nm) in dose 75.5-1510 J/m2 on the structural functional qualities (cytotoxic activity, antibody-produced ability, a level of expression of superficial receptors, a level cAMP) of lymphocytes peripheral blood of donors was investigated. Corregative action of UV-radiation on cytotoxic activity of lymphocytes in relation to cells of Ehrlich ascite carcinoma was revealed. The interrelation of modification types of plasmatic membrane components with a level of functional activity of lymphocytes was established. Was shown, that UV-light induces the decrease in a secondary level of messenger cAMP in cytosol of the photomodified cells. It is revealed, that UV-radiation causes stimulation of antibody-independent mechanism of cytotoxic lymphocytes, which is connected with activation of T-cells and with inhibition of antibody-dependent mechanism of cytotoxic activity.