1.
Chem Commun (Camb)
; 57(37): 4568-4571, 2021 May 07.
Artigo
em Inglês
| MEDLINE
| ID: mdl-33955974
RESUMO
We generated a novel G-quadruplex (G4)-specific endonuclease by fusing a G4 recognition domain of the RHAU helicase with a cleavage domain of the Fok1 nuclease. The fusion protein can specifically bind a parallel G4 and cleave a double-stranded DNA (dsDNA) next to it. The new endonuclease could be used to detect a G4 in a long dsDNA, providing a useful tool for mapping the formation of G4s in the genome.