RESUMO
In this study, we used the next-generation sequencing method to deduce two complete mitogenomes of Woodhead's angelfish (Centropyge woodheadi) and Herald's angelfish (Centropyge heraldi) for the first time. The assembled mitogenome, consisting of 16 863 and 16 836 bp, shows 95.3% identity to each other. Both mitogenome follow a typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop contains 1158 bp in C. woodheadi and 1129 bp in C. heraldi and locates between tRNA-Pro and tRNA-Phe. The overall GC content is 47.9% for C. woodheadi and 47.5% for C. heraldi. The complete mitogenomes of C. woodheadi and C. heraldi provide an essential and important DNA molecular data for phylogenetic and evolutionary analysis for marine angelfish.
Assuntos
Peixes/genética , Genoma Mitocondrial , Animais , Composição de Bases , Evolução Molecular , Proteínas de Peixes/genética , Sequenciamento de Nucleotídeos em Larga Escala , Proteínas Mitocondriais/genética , Filogenia , RNA Ribossômico/genética , RNA de Transferência/genética , Sequenciamento Completo do GenomaRESUMO
In this study, we used the next-generation sequencing method to deduce two complete mitogenomes of Cocos lemonpeel angelfish (Centropyge flavissima) and Eibl's angelfish (Centropyge eibli) for the first time. The assembled mitogenome, consisting of 17 010 bp and 17 101 bp, showing 98.3% identity each other. Both mitogenomes follow the typical vertebrate arrangement, including 13 protein coding genes, 22 transfer RNAs, two ribosomal RNA genes, and a non-coding control region of D-loop. D-loop contains 1302 bp in C. flavissima and 1392 bp in C. eibli and locates between tRNA-Pro and tRNA-Phe. The overall GC content is 45.2% for C. flavissima and 44.9% for C. eibli. Complete mitogenomes of C. flavissima and C. eibli provide essential and important DNA molecular data for phylogenetic and evolutionary analysis for marine angelfishes.
Assuntos
Genoma Mitocondrial , Perciformes/genética , Animais , Composição de Bases , Proteínas de Peixes/genética , Perciformes/classificação , Filogenia , RNA Ribossômico/genética , RNA de Transferência/genética , Homologia de SequênciaRESUMO
In this study, we used the next-generation sequencing method to deduce two complete mitogenomes of Barred angelfish (Paracentropyge multifasciata) and Purplemask angelfish (Paracentropyge venusta) for the first time. The assembled mitogenome, consisting of 16 502 bp and 16 641 bp, showing 88.6% identity each other. Both mitogenome follow the typical vertebrate arrangement, including 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop contains 787 bp in P. multifasciata and 923 bp in P. venusta and locates between tRNA-Pro and tRNA-Phe. The overall GC content is 46.5% for P. multifasciata and 46.2% for P. venusta. Complete mitogenomes of P. multifasciata and P. venusta provide essential and important DNA molecular data for phylogenetic and evolutionary analysis for marine angelfish.
Assuntos
Peixes/classificação , Peixes/genética , Genoma Mitocondrial , Animais , Composição de Bases , Genes Mitocondriais , Tamanho do Genoma , Fases de Leitura Aberta , Filogenia , Análise de Sequência de DNA , Sequenciamento Completo do GenomaRESUMO
Whitetail Angelfish (Centropyge flavicauda) and Orangeback Angelfish (Centropyge acanthops) are known as pygmy angelfish due to their small body size (usually less than two inches). In this study, we used the next-generation sequencing method to deduce two complete mitogenomes of C. flavicauda and C. acanthops for the first time. The assembled mitogenome, consisting of 16 747 bp and 16 748 bp, was showing 99.2% identity each other. Both mitogenome follow the typical vertebrate arrangement, including 13 protein coding genes, 22 transfer RNAs, two ribosomal RNAs genes and a non-coding control region of D-loop. D-loop contains 895 bp in C. flavicauda and 896 bp in C. acanthops and locates between tRNA-Pro and tRNA-Phe. The overall GC content is 46.4% for C. flavicauda and C. acanthops. Complete mitogenomes of C. flavicauda and C. acanthops provide essential and important DNA molecular data for phylogenetic and evolutionary analysis for marine angelfish.
Assuntos
Peixes/classificação , Peixes/genética , Genoma Mitocondrial , Animais , Composição de Bases , Rearranjo Gênico , Genes Mitocondriais , Tamanho do Genoma , Fases de Leitura Aberta , Filogenia , Análise de Sequência de DNA , Sequenciamento Completo do GenomaRESUMO
In this study, the complete mitogenome sequence of the cryptic "lineage A" big-fin reef squid, Sepioteuthis lessoniana (Cephalopoda: Loliginidae) has been sequenced by the next-generation sequencing method. The assembled mitogenome consists of 16,605 bp, which includes 13 protein-coding genes, 22 transfer RNAs, and 2 ribosomal RNAs genes. The overall base composition of "lineage A" S. lessoniana is 37.5% for A, 17.4% for C, 9.1% for G, and 35.9% for T and shows 87% identities to "lineage C" S. lessoniana. It is also noticed by its high T + A content (73.4%), two non-coding regions with TA tandem repeats. The complete mitogenome of the cryptic "lineage A" S. lessoniana provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for big-fin reef squid species complex.
Assuntos
Decapodiformes/classificação , Decapodiformes/genética , Genoma Mitocondrial , Animais , Composição de Bases , Códon , Evolução Molecular , Genes Mitocondriais , Tamanho do Genoma , Fases de Leitura Aberta , Filogenia , Análise de Sequência de DNA , Sequenciamento Completo do GenomaRESUMO
In this study, we used the next-generation sequencing method to determine the complete mitogenome of Japanese swallow angelfish (Genicanthus semifasciatus) and Ornate angelfish (Genicanthus bellus) for the first time. The assembled mitogenome, 16,722 bp and 16,734 bp in length, shows 95% identity to each other. Both mitogenomes follow the typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop contains length of 1013 bp in G. semifasciatus and 1030 bp in G. bellus, and is located between tRNA-Pro and tRNA-Phe. The overall GC content is 46.4% for G. semifasciatus and 46.6% for G. bellus. The complete mitogenome of Japanese swallow angelfish and Ornate angelfish determined in this study provides essential and important DNA molecular data for further phylogenetic and evolutionary analyses for marine angelfish.
Assuntos
Cipriniformes/genética , Genoma Mitocondrial/genética , Animais , Composição de Bases/genética , Cipriniformes/classificação , Ordem dos Genes/genética , Sequenciamento de Nucleotídeos em Larga Escala , Filogenia , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
The Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) are distributed in the Indian and West Pacific Ocean and considered as complex species based on morphological and molecular evidences. In this study, we used the next-generation sequencing method to decode two complete mitogenomes of H. leoparda and N. kuhlii. The assembled mitogenome, consisting lengths of 17,690 bp for H. leoparda and 17,974 bp for N. kuhlii, shows 78% identity to each other. Both mitogenomes follow the typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop with the lengths 1931 bp (H. leoparda) and 2243 bp (N. kuhlii) is located between tRNA-Pro and tRNA-Phe. The overall GC content is 40.3% for H. leoparda and 39.8% for N. kuhlii. The complete mitogenome of H. leoparda and N. kuhlii provides essential and important DNA molecular data for further phylogenetic and evolutionary analyses for stingray species complex.
Assuntos
Genoma Mitocondrial/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Rajidae , Animais , Composição de Bases/genética , Filogenia , RNA Ribossômico/genética , RNA de Transferência/genética , Rajidae/genéticaRESUMO
In this study, we used the next-generation sequencing method to deduce two complete mitogenomes of Spotbreast angelfish (Genicanthus melanospilos) and Blackstriped angelfish (Genicanthus lamarck) for the first time. The assembled mitogenome, consisting of 16 736 bp and 16 616 bp, showing 91.8% identity each other. Both mitogenome follow the typical vertebrate arrangement, including 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop contains 1026 bp in G. melanospilos and 912 bp in G. lamarck and locates between tRNA-Pro and tRNA-Phe. The overall GC content is 46.2% for G. melanospilos and 46.3% for G. lamarck. Complete mitogenomes of G. melanospilos and G. lamarck provide essential and important DNA molecular data for phylogenetic and evolutionary analysis for marine angelfishes.
Assuntos
Peixes/classificação , Peixes/genética , Genoma Mitocondrial , Animais , Composição de Bases , Genes Mitocondriais , Tamanho do Genoma , Fases de Leitura Aberta , Filogenia , Análise de Sequência de DNA , Sequenciamento Completo do GenomaRESUMO
In this study, the complete mitogenome sequence of largescale mullet (Teleostei: Mugilidae) has been sequenced by the next-generation sequencing method. The assembled mitogenome, consisting of 16,832 bp, had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs genes, and a non-coding control region of D-loop. D-loop which has a length of 1094 bp is located between tRNA-Pro and tRNA-Phe. The overall base composition of largescale mullet is 27.8% for A, 30.1% for C, 16.2% for G, and 25.9% for T. The complete mitogenome may provide essential and important DNA molecular data for further phylogenetic and evolutionary analysis for Mugilidae.
