[Features of assay and decomposition dynamics of 2-methoxy-4-(1-propenyl)hydroxybenzene in biological material]. / Osobennosti opredeleniya i dinamiki razlozheniya 2-metoksi-4-(1-propenil)gidroksibenzola v biologicheskom materiale.

The objective was to study the features of assay and dynamics of decomposition of 2-methoxy-4-(1-propenyl)hydroxybenzene in biological material. Extraction, semi-preparation chromatography, TLC, HPLC, GC-MS and UV-spectrophotometry were used as test methods. 2-Methoxy-4-(1-propenyl)hydroxybenzene was extracted from the biological material by double infusion (45 min each) with ethyl acetate at a 2:1 mass ratio of isolating agent and biomatrix. Purification was performed by extraction and chromatography in a semi-preparative (190×10 mm) L 40/100 µm silica gel column using a hexane-dioxane (7:3) eluent. The analyte was determined by TLC methods (Sorbfil plates, hexane-acetone 9:1 as a mobile phase), HPLC [Discovery C18 HPLC Column (250×4.6 mm), acetonitrile-acetate buffer pH 5.5 (5:5) as a mobile phase], GC-MS [DB-5MS EVIDEX (25 m×0.2 mm) column with 5%-phenyl-95% dimethyl polysiloxane as a stationary phase], UV-spectrophotometry (95% ethanol as a solvent). The proposed assay method for 2-methoxy-4-(1-propenyl)hydroxybenzene in biomaterial (liver tissue) is validated for linearity, selectivity, accuracy and precision. The study results showed that the decomposition rate of the analyte increases as the store temperature increases. At 0-2 °C, 8-10 °C and 18-22 °C 2-methoxy-4-(1-propenyl)hydroxybenzene is stable for 480, 390 and 260 days respectively.

[The specific features of the distribution of 2,4- and 2,6-di--butylhydroxybenzenes on the body of the warm-blooded animals]. / Osobennosti raspredeleniia 2,4- i 2,6-di--butilgidroksibenzola v organizme teplokrovnykh zhivotnykh.

The present study was designed to elucidate the character of the distribution of 2,4- and 2,6-di-tret-butylhydroxybenzenes (2,4-DTBHOB and 2,6-DTBHOB respectively) in the body of the warm-blooded animals (rats) following the administration of the three-fold LD50 dose into the stomach. Both 2,4-DTBHOB and 2,6-DTBHOB were extracted from the blood and the organs of the perished animals by means of two-fold incubation of the sampled tissues in ethyl acetate with the subsequent purification of the isolates by passing the extracts through a L 40/100 mcm silicagel column using hexane:dioxane (8.5:1.5) for 2,4-DTBHOB and hexane:dioxane (97.5:2.5) for 2,6-DTBHOB as eluants. The compounds of interest were identified and quantified by means of TLC, HPLC, and UV-spectrometry. The study has shown that both 2,4-DTBHOB and 2,6-DTBHOB were present in the organs and blood of the poisoned animals in the unmetabolized form. Their largest amounts (mg/100 g) were found in the contents of the stomach, the small intestines with the contents and in the spleen.

[The peculiar features of the distribution of 2-metoxy-4-(1-propenyl)-hydroxybenzene in the organism of the warm-blooded animals]. / Osobennosti raspredeleniia 2-metoksi-4-(1-propenil)-gidroksibenzola v organizme teplokrovnykh zhivotnykh.

The present study was designed to elucidate the distribution patterns of 2-metoxy-4-(1-propenyl)-hydroxybenzene in the organism of the warm-blooded animals (rats) after its intragastric administration at a dose equivalent to three 50% lethal doses (LD-50) for this compound. 2-metoxy-4-(1-propenyl)-hydroxybenzene was extracted in the unmodified form from the organs and blood of the dead animals after the dualfold infusion of the tissues with ethyl-acetate and purification by chromatography on a macrocolumn with silica gel L 40/100 mcm making use of the hexane-acetone mixture (7:3) mobile phase as the eluent. TLC, HPLC, and UV-spectrometry were employed to identify and quantify the analyte. The study has demonstrated the presence of unmetabolized 2-metoxy-4-(1-propenyl)-hydroxybenzene in the organs and blood of the poisoned animals. The largest amounts of this compound (expressed in milligrams per 100 g of the tissue) were accumulated in the stomach with its contents (236.22±28.21), small intestine with their contents (122.29±15.55), lungs (44.28±2.10), and spleen (44.00±4.70).

[The determination of 2,4-di-tret-butyl hydroxybenzene for the purpose of the chemical toxicological study of the biological materials]. / Opredelenie 2,4-di-tret-butilgidroksibenzola pri khimiko-toksikologicheskom issledovanii biologicheskogo materiala.

The objective of this work was to study peculiarities of identification of 2,4-di-tert-butyl hydroxybenzene (2,4-DTBHOB) in blood and the tissues of various organs with the use TLC, UR- and UV-spectrophotometry. The results of the study suggest the expediency of the application of ethylacetate as the extractive agent for the isolation of 2,4-DTBHOB from the biological materials. The optimal conditions for 2,4-DTBHOB extraction by this method were developed based on the decontamination of the analyte by means of removal of the organic substances of biological matrices using the L 40/100 mcm sorbent columns with the hexan/dioxane mixture (8.5:1.5) as the mobile phase. The method for the determination of 2,4-di-tert-butyl hydroxybenzene in the tissues of various organs (namely, liver) and blood has been developed. The proposed method allows to determine the minimal quantities of 2,4-DTBHOB present in the biological objects equivalent to 0.36 mg and 0.28 mg in the hepatic tissue and blood respectively.

[The peculiarities of determination of 2-chloro-1,4-dihydrooxybenzene in the biological material]. / Osobennosti opredeleniya 2-khlor-1,4-digidroksibenzola v biologicheskom materiale.

The objective of the present study was to elucidate the peculiarities of the determination of 2-chloro-1,4-dihydrooxybenzene in the tissues of different organs and blood. The analytical methods used in the study included TLC, GC-MS, and UF-spectrophotometry. The study has demonstrated the usefulness of using acetone as an insulating solvent for the extraction of 2-chloro-1,4-dihydrooxybenzene from the biological materials. It was shown that the compound being analyzed can be separated from the endogenous substances contained in the biological matrices on the "Silasorb C-18" columns, 30 mcm. The method for the identification of 2-chloro-1,4-dihydrooxybenzene in the blood and organ tissues (such as normal and putrefied renal tissues) was developed. The method permits to determine 0.26 mg and 0.30 mg of the substance of interest in 100 g of the normal and putrefied renal tissues respectively; in addition, it measures 0.22 mg of 2-chloro-1,4-dihydrooxybenzene in the blood.

[The specific features of the distribution of 4-metoxyhydroxybenzene in the organism of the warm-blooded animals suffering lethal intoxication]. / Osobennosti raspredeleniya 4-metoksigidroksibenzola v organizme teplokrovnykh zhivotnykh pri letal'nykh otravleniyakh.

This work was designed to study the distribution of 4-metoxyhydroxybenzene in the organism of the omnivorous warm-blooded animals (rats) after the intragastric administration of this poisonous compound at a dose three-fold greater than the LD50 value. The administered 4-metoxyhydroxybenzene was isolated from the organs and blood of the experimental animals by exposing the biological tissues to acetone with subsequent purification on a silica gel L 40/100 mcm using a hexane:dioxane:propanol-2 (20:5:1) as the mobile phase. The identification and quantitation of 4-metoxyhydroxybenzene were carried out with the use of TLC, GC-MS, and UF-spectrophotometry. It was shown that the administered 4-metoxyhydroxybenzene remained unmetabolized in the internal organs and blood of the poisoned experimental animals. The largest amounts of 4-metoxyhydroxybenzene were found in the stomach contents (2584,92±117,47), brain (59.49±6.05), contents of small intestines (28.21±3.77), and kidneys (26.13±1.64).

[The specific features of the distribution of 2,6-di-tret-buthyl-4-methlhydroxybenzole in the organism of the warm-blooded animals].

We have studied the specific features of the distribution of 2,6-di-tret-buthyl-4-methlhydroxybenzole in the organism of the omnivorous warm-blooded animals (rats) following the intragastric administration of the three-fold lethal dose of the poisonous substance. 2,6-di-tret-buthyl-4-methlhydroxybenzole was isolated from the blood and various organs of the animals by means of acetone extraction. It was further purified on the 40/100 mcm L silicagel column with the use of hexane/acetone for elution. TLC, GC-MS, and UV-spectrophotometry were employed to identify and quantify the material of interest. It was shown that 2,6-di-tret-buthyl-4-methlhydroxybenzole undergoes modification in the internal organs and blood of the poisoned animals. In was present in the highest amounts (100 mg/g) in the stomach and small intestine contents (787.78±52.31 and 18.31±3.47 respectively), spleen (12.46±1.02), and kidneys (8.48±0.61).