[Beta-galactosidase of the superthermophilic anaerobic bacteria Thermoanaerobium sp. 2905 and its immobilization]. / Beta-galaktozidaza supertermofil'noi anaérobnoi bakterii Thermoanaerobium sp. 2905 i ee immobilizatsiia.

The composition of nutrition medium for cultivating the extreme thermophilic anaerobe Thermoanaerobium sp. 2905 was optimized, which enabled the bacterial beta-galactosidase production to be substantially enhanced. Xylan and ammonium phosphate were selected as optimal carbon and nitrogen sources, respectively. The enzyme was purified fivefold by precipitation of the aqueous cell extract with alcohol (1:1, v/v), and crude preparation with a specific activity of 46 U per mg protein was obtained. Cells of the extreme thermophile were entrapped into natural or synthetic latex.

[Optimization of the conditions for beta-galactosidase biosynthesis in eukaryotes]. / Optimizatsiia uslovii biosinteza beta-galaktozidavy u éukariotov.

Liquid whey can be subsituted by dry whey in the growth medium for Saccharomyces fragilis producing endocellular beta-galactosidase. The total biosynthesis of beta-galactosidase by the yeast on the medium containing dry whey can be increased by 40-50 percent as a result of additional stepwise introduction of lactose into the medium or optimization of the medium by mathematical planning of the experiment. Constructive metabolism of the yeast is not correlated with the rate of biosynthesis of beta-galactosidase. Damages in constructive metabolism of facultative anaerobes - yeast cultures - caused by the limitation of aeration result in an increase of the rate of beta-galactosidase biosynthesis. Such a correlation between the rate of the enzyme biosynthesis and the degree of aeration of the culture is not found in strict aerobes - fungi Alternaria tenuis and Curvularia inaequalis.