RESUMO
We tested by electron microscopy 7,530 samples from children admitted to different hospitals in Sofia between February 1981 and February 1986; from these, rotaviruses were found in 725 (9.6%) faecal samples. Electron microscopic analysis of 264 samples from 181 children admitted to the hospital of infectious diseases in Shumen between December 1984 to February 1986 revealed rotaviruses in 120 (66.6%) of the tested children. A part of the samples positive by electron microscopy was tested by ELISA and polyacrylamide gel electrophoresis for rotavirus RNA segment patterns. Rotaviruses with eight different electropherotypes were found in Bulgaria. The seasonal culmination of the rotavirus gastroenteritis in the winter months has been confirmed. Rotavirus antibodies were found in 73% of the sera from 152 children tested.
Assuntos
Gastroenterite/epidemiologia , Infecções por Rotavirus/epidemiologia , Bulgária , Criança , Pré-Escolar , Métodos Epidemiológicos , Fezes/microbiologia , Gastroenterite/etiologia , Humanos , Lactente , Recém-Nascido , Microscopia Eletrônica , Rotavirus/isolamento & purificação , Estações do AnoRESUMO
The incorporation of labelled precursors of DNA, RNA and proteins in F. cells infected with Coxsackie B4 virus has been traced both in the presence and absence of total histone from nucleated erythrocytes during the infectious process. In comparison with the virus infection alone, the exogenous histone supressed the incorporation of 3H-thymidine in early hours and the incorporation of 3H-uridine and 14C-leucine in the 9th hour after virus infection. The established repression of the incorporation of the labelled precurssors for RNA and proteins correlates with a drop in the order of 90 to 99% in the yield of infectious virus.
