RESUMO
We have developed an optical telemetry system for recording electrical signals associated with muscle and neuronal activities from freely walking crayfish under water. The device was made from conventional electronic parts which are commercially available, utilizing infrared light (880 nm) for signal transmission. Two or four channels of biological signals were multiplexed, the voltage of each data point modulated to the duration of subcarrier pulses and further to the interval of narrower carrier pulses that directly drove the infrared light emission diode (IRLED) under water. The light-pulse modulated signals were received by photodiodes and demodulated to restore the original two or four channel signals. Electrical recordings using wired electrodes and conventional amplifiers revealed that the optically transmitted signals were consistent with the wire-transmitted ones. In order to test the performance of this system, we recorded electromyograms (EMGs) from the second and third walking legs on each side of crayfish together with the neuronal activity in the ventral nerve cord. The results confirmed our previous observation in tethered crayfish that the background tonus of leg muscles showed an increase preceding their rhythmic activation.
