RESUMO
Protease-targeted inhibitors have been promising pharmaceuticals. Here, we combined a yeast cell surface display system with a yeast cell chip for the high-throughput screening of protease inhibitors, and succeeded in improving the activity of a protease inhibitor.
Assuntos
Ensaios de Triagem em Larga Escala/métodos , Inibidores de Proteases/farmacologia , Avaliação Pré-Clínica de Medicamentos , Cadeias Leves de Imunoglobulina/química , Metaloendopeptidases/antagonistas & inibidores , Proteínas Recombinantes de Fusão/farmacologia , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/metabolismoRESUMO
Silica-based one-dimensional photonic crystal (1D-PC) is fabricated by use of a high-spatial-frequency grating with input and output surfaces tilted with respect to its periodic direction. An incident beam is coupled with the first photonic band in the second Brillouin zone of the 1D-PC. The output beam angle changes 3 degrees with a wavelength change of 1%. A prototype of an ultrasmall demultiplexer is demonstrated by use of a silica slab waveguide with 1D-PC.
RESUMO
A compact and monolithic four-channel demultiplexer with 20-nm spacing was fabricated by use of a high-spatial-frequency transmission grating buried in a silica waveguide. The grating was successfully buried in a silica slab waveguide by overcladding by plasma-enhanced chemical-vapor deposition. The device's size was approximately 5.1 mm x 9.2 mm. The average insertion loss was 13 dB. The average cross talk was -15 dB for adjacent channels and -19 dB for nonadjacent channels. The polarization-dependent loss was estimated to be as low as 0.7 dB.
