Assuntos
Psoríase , Humanos , Doença Aguda , Causalidade , Doença Crônica , Psoríase/epidemiologia , Psoríase/genética , Pirina/genéticaAssuntos
Interleucina-8/sangue , Linfoma Anaplásico de Células Grandes/patologia , Neutrófilos/patologia , Neoplasias Cutâneas/patologia , Adulto , Humanos , Antígeno Ki-1/metabolismo , Linfócitos/metabolismo , Linfoma Anaplásico de Células Grandes/sangue , Masculino , Regressão Neoplásica Espontânea , Neutrófilos/metabolismo , Neoplasias Cutâneas/sangueRESUMO
BACKGROUND: Rhododendrol (rhododenol), an inhibitor of tyrosinase activity, is used as a skin-whitening component. Many cases of leukoderma after the application have been reported, termed rhododenol-induced leukoderma (RIL). The aim of this study was to clarify the pathogenesis of RIL morphologically through comparison with vitiligo. METHODS: We examined 14 cases of RIL and 15 cases of vitiligo using routine histopathology and immunohistochemistry. Thirteen cases of RIL, six cases of vitiligo and specimens of the RIL mouse model were evaluated by electron microscopy. RESULTS: There were common findings in RIL and vitiligo at the light-microscopic level: (a) vacuolar changes in the dermo-epidermal junction, (b) melanophages in the papillary dermis, (c) perifollicular lymphocyte infiltration, (d) loss or decrease of basal melanin pigment and (e) decrease of melanocytes in the lesions. The ultrastructural observations showed specific findings of RIL: (a) remaining melanocytes in depigmented lesions, (b) inhomogeneous melanization in melanocytes and (c) degenerated melanosomes in melanocytes. Some of the findings were observed in a RIL mouse model. Furthermore, it is notable that cell organelles of melanocytes were intact in our RIL cases. CONCLUSION: Morphological changes of RIL targeting melanosomes in melanocytes without degeneration of organelles reflect the reversible clinical course of most cases.
Assuntos
Butanóis/efeitos adversos , Melanócitos , Nevo , Neoplasias Cutâneas , Vitiligo/tratamento farmacológico , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Butanóis/administração & dosagem , Feminino , Humanos , Melanócitos/metabolismo , Melanócitos/patologia , Camundongos , Pessoa de Meia-Idade , Neoplasias Experimentais/induzido quimicamente , Neoplasias Experimentais/metabolismo , Neoplasias Experimentais/patologia , Nevo/induzido quimicamente , Nevo/metabolismo , Nevo/patologia , Neoplasias Cutâneas/induzido quimicamente , Neoplasias Cutâneas/metabolismo , Neoplasias Cutâneas/patologia , Vitiligo/metabolismo , Vitiligo/patologiaAssuntos
Pustulose Exantematosa Aguda Generalizada/etiologia , Extratos Vegetais/efeitos adversos , Pustulose Exantematosa Aguda Generalizada/diagnóstico , Pustulose Exantematosa Aguda Generalizada/patologia , Adulto , Feminino , Humanos , Ativação Linfocitária , Panax , Testes do Emplastro , Pele/patologia , Zanthoxylum , ZingiberaceaeRESUMO
We report a case of adenocarcinoma affecting the chin of a 48-year-old man. The tumor showed signs of apocrine differentiation and had infiltrated the muscle. The patient had no history or clinical evidence of breast cancer. We made a diagnosis of cutaneous apocrine adenocarcinoma. Apocrine adenocarcinoma rarely arises in areas with scarce apocrine glands. We reviewed the literature on apocrine adenocarcinoma of the face in areas other than the eyelids and auditory canal, where specialized apocrine glands are present.
Assuntos
Dermatite Esfoliativa/tratamento farmacológico , Fármacos Dermatológicos/uso terapêutico , Toxidermias/tratamento farmacológico , Infliximab/uso terapêutico , Idoso , Aminoquinolinas/efeitos adversos , Antineoplásicos/efeitos adversos , Dermatite Esfoliativa/induzido quimicamente , Toxidermias/etiologia , Feminino , Humanos , ImiquimodeAssuntos
Adenocarcinoma Mucinoso/patologia , Neoplasias Primárias Múltiplas/patologia , Neoplasias Primárias Desconhecidas/patologia , Doença de Paget Extramamária/patologia , Idoso , Neoplasias do Ânus/química , Neoplasias do Ânus/patologia , Feminino , Humanos , Neoplasias Primárias Múltiplas/química , Neoplasias Primárias Desconhecidas/química , Doença de Paget Extramamária/química , Neoplasias Cutâneas/química , Neoplasias Cutâneas/patologia , Neoplasias Uretrais/química , Neoplasias Uretrais/patologia , Neoplasias da Bexiga Urinária/química , Neoplasias da Bexiga Urinária/patologia , Neoplasias do Colo do Útero/química , Neoplasias do Colo do Útero/patologia , Neoplasias Vulvares/química , Neoplasias Vulvares/patologiaAssuntos
Artrite Reumatoide/tratamento farmacológico , DNA Bacteriano/genética , Glucocorticoides/efeitos adversos , Técnicas de Diagnóstico Molecular , Infecções por Mycobacterium não Tuberculosas/diagnóstico , Mycobacterium chelonae/genética , Mycobacterium chelonae/isolamento & purificação , Infecções Oportunistas/diagnóstico , Dermatopatias Bacterianas/diagnóstico , Pele/microbiologia , Idoso , Antibacterianos/uso terapêutico , Artrite Reumatoide/diagnóstico , Artrite Reumatoide/imunologia , Biópsia , Quimioterapia Combinada , Feminino , Humanos , Hospedeiro Imunocomprometido , Infecções por Mycobacterium não Tuberculosas/tratamento farmacológico , Infecções por Mycobacterium não Tuberculosas/imunologia , Infecções por Mycobacterium não Tuberculosas/microbiologia , Mycobacterium chelonae/efeitos dos fármacos , Mycobacterium chelonae/imunologia , Infecções Oportunistas/tratamento farmacológico , Infecções Oportunistas/imunologia , Infecções Oportunistas/microbiologia , Reação em Cadeia da Polimerase , Valor Preditivo dos Testes , Ribotipagem , Pele/efeitos dos fármacos , Pele/imunologia , Pele/patologia , Dermatopatias Bacterianas/tratamento farmacológico , Dermatopatias Bacterianas/imunologia , Dermatopatias Bacterianas/microbiologiaRESUMO
There is currently an urgent need to develop safe and effective adjuvants for enhancing vaccine-induced antigen-specific immune responses. We demonstrate here that intranasal immunization with clinically used polypeptide antibiotics, polymyxin B (PMB) and colistin (CL), along with ovalbumin (OVA), increases OVA-specific humoral immune responses in a dose-dependently manner at both mucosal and systemic compartments. Enhanced immunity by boosting was found to persist during 8 months of observation. Moreover, mice intranasally immunized with OVA plus various doses of PMB or CL showed neither inflammatory responses in the nasal cavity and olfactory bulbs nor renal damages, compared to those given OVA alone. These data suggest that polymyxins may serve as novel and safe mucosal adjuvants to induce humoral immune responses. The polymyxin adjuvanticity was found to be independent of endotoxins liberated by its bactericidal activity, as indicated by similar enhancing effects of PMB in lipopolysaccharide (LPS)-hyporesponsive and LPS-susceptible mice. However, despite the presence of preexisting anti-PMB antibodies, we observed no reduction in the adjuvant function of polymyxins when they were given intranasally. Furthermore, the titers of OVA-specific Abs in mice intranasally immunized with OVA plus PMB or CL were significantly higher than those in mice administered with polymyxin analogues, such as polymyxin B nonapeptide and colistin methanesulfonate. The levels of released ß-hexosaminidase and histamine in mast cell culture supernatants stimulated by PMB or CL were also significantly higher than those stimulated by their analogues. These results suggest that both the hydrophobic carbon chain and hydrophilic cationic cyclic peptide contribute to the mucosal adjuvanticity of PMB and CL.
Assuntos
Adjuvantes Imunológicos/farmacologia , Colistina/farmacologia , Imunidade Humoral/efeitos dos fármacos , Ovalbumina/farmacologia , Polimixina B/farmacologia , Adjuvantes Imunológicos/administração & dosagem , Administração Intranasal , Animais , Colistina/administração & dosagem , Relação Dose-Resposta a Droga , Ensaio de Imunoadsorção Enzimática , Feminino , Histamina/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Ovalbumina/administração & dosagem , Polimixina B/administração & dosagem , Estatísticas não Paramétricas , beta-N-Acetil-Hexosaminidases/metabolismoRESUMO
Solar lentigines (SL) are hyperpigmented lesions generally seen in elderly people. Their pathogenesis has not been completely elucidated. We examined 75 cases of SL using routine histopathology and immunohistochemistry. In addition, seven cases were evaluated by electron microscopy. Histopathologically, we observed vacuolar changes in the dermoepidermal junction in 85% of the cases. Dermal melanophages were seen in 77% of the cases. The immunohistochemical expression rates in the epidermis for cytokeratin (CK)15, CK14, CK10, p63 and nestin were 76%, 100%, 100%, 100% and 17%, respectively. In 58 cases showing dermal melanophages, expression rates of CD163 and factor XIIIa on melanophages were 79% and 83%, respectively. Double positivity for both proteins was identified in 44 cases (75%). Ultrastructurally, vacuolar structures were seen in the cytoplasm of basal cells and upper dermis in all cases examined. We observed elimination processes of damaged basal keratinocytes, which were probably produced by ultraviolet (UV) irradiation, into the papillary dermis. The segregated damaged cell bodies containing melanin granules seemed to be phagocytosed by poorly immunostimulatory macrophages labeled immunohistochemically by CD163 and factor X IIIa, contributing to prolonged pigmentation of SL. In addition, repeated basal keratinocyte damages may be in association with altered CK and p63 expression patterns in the constituent cells of SL.