RESUMO
OBJECTIVE: To observe the ultrastructure of bone marrow stromal cells (BMSCs) cultured in coralline hydroxyapatite (CHA) and evaluate their biocompatibility. METHODS: BMSCs isolated from dogs were cultured with CHA as the scaffold, and the morphologies of the cells were observed with phase-contrast microscope and scanning electron microscope. RESULTS AND CONCLUSION: BMSCs grew well with good attachment to the CHA scaffold and performed normal function, demonstrating CHA as one of useful biocarrier materials for bone tissue engineering.
Assuntos
Células da Medula Óssea/citologia , Cerâmica/química , Hidroxiapatitas/química , Células Estromais/citologia , Animais , Células da Medula Óssea/ultraestrutura , Substitutos Ósseos/química , Técnicas de Cultura de Células , Células Cultivadas , Cães , Masculino , Microscopia Eletrônica de Varredura , Microscopia de Contraste de Fase , Células Estromais/ultraestrutura , Engenharia Tecidual/métodos , Alicerces Teciduais/químicaRESUMO
OBJECTIVE: To induce the differentiation of bone marrow stromall cells (BMSCs) isolated from Beagles into osteoblasts in vitro and identify the osteogenic potential and bioactivity of the BMSCs. METHODS: Primary cultured BMSCs isolated from Beagles were subcultured in mineralization medium to induce their differentiation into osteoblasts, whose morphological characteristics and proliferation status were observed by phase-contrast microscope. The osteogenic activity of the cells was evaluated with von Kossa staining of the mineralized nodules and determination of the alkaline phosphatase activity. RESULT: BMSCs cultured in vitro showed obvious osteogenic capacity in DMEM. Von Kossa staining of the mineralized nodules and alkaline phosphatase detection of the passaged cells both yielded positive results. CONCLUSION: BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.
