Assuntos
Cromatos/intoxicação , Glutationa Transferase/urina , Rim/efeitos dos fármacos , Intoxicação por Mercúrio/urina , Dicromato de Potássio/intoxicação , Toxinas Biológicas , Animais , Glutationa Transferase/sangue , Glutationa Transferase/metabolismo , Rim/patologia , Masculino , Radioimunoensaio , Ratos , Uremia/metabolismoRESUMO
The development of sensitive and specific radioimmunoassay for ligandin, a protein present in abundance in the hepatic cytoplasm, has permitted measurement of ligandin in rat plasma. Plasma ligandin levels were estimated in normal, carbon tetrachloride (CCl4)-treated, and bile duct-ligated rats, with parallel estimation of serum glutamic oxaloacetic transaminase (SGOT). Plasma ligandin levels rose at a greater rate and to higher levels than did SGOT in CCl4-treated rats and showed a more linear increment to increasing doses of CCl4. A marked depletion of hepatic, but not renal ligandin, was associated with CCl4 poisoning. Twelve hours after acute bile duct ligation, both plasma ligandin and SGOT were moderately elevated, plasma ligandin returning to normal by 24 hr, at which time SGOT was still raised. The findings of this study indicate that plasma ligandin is a sensitive index of experimental hepatocellular necrosis.
Assuntos
Intoxicação por Tetracloreto de Carbono/diagnóstico , Doença Hepática Induzida por Substâncias e Drogas/diagnóstico , Glutationa Transferase/sangue , Cirrose Hepática Biliar/diagnóstico , Radioimunoensaio/métodos , Animais , Aspartato Aminotransferases/sangue , Coleta de Amostras Sanguíneas , Tetracloreto de Carbono/administração & dosagem , Intoxicação por Tetracloreto de Carbono/sangue , Doença Hepática Induzida por Substâncias e Drogas/sangue , Cromatografia em Gel , Ducto Colédoco/cirurgia , Relação Dose-Resposta a Droga , Estudos de Avaliação como Assunto , Ligadura , Cirrose Hepática Biliar/sangue , Masculino , Necrose , Ratos , Fatores de TempoAssuntos
Glutationa Transferase/análise , Animais , Animais Recém-Nascidos/metabolismo , Especificidade de Anticorpos , Cromatografia em Gel , Feminino , Glutationa Transferase/imunologia , Glutationa Transferase/metabolismo , Rim/metabolismo , Fígado/metabolismo , Masculino , Fenobarbital/farmacologia , Radioimunoensaio/métodos , Ratos , Distribuição TecidualRESUMO
Purified ligandin (Y-protein) a 46000-dalton protein, has been shown to consist of two subunit species (mol. wts. 22 000 and 24 000) on discontinuous polyacrylamide gel electrophoresis in sodium dodecyl sulphate. This technique was used to define further the nature of these subunits. The Y sulphobromophthalein-binding fraction of rat hepatic cytosol was shown to contain three major subunit bands designated subunit Ya, subunit Yb and subunit Yc in ascending order of size. Purified ligandin was found to comprise Ya and Yc subunit species, and also gave two bands on isoelectric focusing. The two subunit species in purified ligandin were partially separated by an additional purification step. Antiserum to ligandin reacted mono-specifically with the purified protein, as well as hepatic, renal and small intestinal mucosa cytosol, but gave lines of identity and partial identity with cytosol from testis, ovary and adrenal gland. The Y fraction of testis was found to contain only Yb and Yc species, while all three major bands were found in liver, kidney and small intestinal mucosa. Phenobarbital treatment increased the concentration of Ya and Yb in the liver, but had little effect on Yc. These findings suggest that the Ya and Yc ligandin subunits are the monomers of two proteins: YaYa and YcYc.
