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1.
Ecotoxicol Environ Saf ; 188: 109912, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31706240

RESUMO

Synthetic progestins are emerging contaminants of the aquatic environment with endocrine disrupting potential. The main aim of the present study was to investigate the effects of the synthetic progestins gestodene, and drospirenone on sex differentiation in common carp (Cyprinus carpio) by histological analysis. To gain insights into the mechanisms behind the observations from the in vivo experiment on sex differentiation, we analyzed expression of genes involved in hypothalamus-pituitary-gonad (HPG) and hypothalamus-pituitary-thyroid (HPT) axes, histology of hepatopancreas, and in vitro bioassays. Carp were continuously exposed to concentrations of 2 ng/L of single progestins (gestodene or drospirenone) or to their mixture at concentration 2 ng/L of each. The exposure started 24 h after fertilization of eggs and concluded 160 days post-hatching. Our results showed that exposure of common carp to a binary mixture of drospirenone and gestodene caused increased incidence of intersex (32%) when compared to clean water and solvent control groups (both 3%). Intersex most probably was induced by a combination of multiple modes of action of the studied substances, namely anti-gonadotropic activity, interference with androgen receptor, and potentially also with HPT axis or estrogen receptor.


Assuntos
Androstenos/toxicidade , Carpas/crescimento & desenvolvimento , Disruptores Endócrinos/toxicidade , Norpregnenos/toxicidade , Diferenciação Sexual/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Relação Dose-Resposta a Droga , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Gônadas/efeitos dos fármacos , Hepatopâncreas/efeitos dos fármacos , Hipotálamo/efeitos dos fármacos , Hipófise/efeitos dos fármacos , Diferenciação Sexual/genética
2.
Environ Toxicol Pharmacol ; 67: 87-93, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30769281

RESUMO

The purpose of our experiment was to evaluate the effect of enrofloxacin on biotransformation, oxidative stress and mRNA expression of related genes in fish as a non-target organisms. Zebrafish (Danio rerio) juveniles were treated with enrofloxacin at concentrations of 5, 10 and 500 µg/L for 14 days. A three-day-long test caused changes of catalytic activity of glutathione peroxidase and glutathione-S-transferase. Moreover, lipid peroxidation was observed at the highest concentration. No significant changes either in catalytic activity of antioxidant enzymes or elevated lipid peroxidation were observed from sampling day 7 on. mRNA expression of genes encoding antioxidant enzymes was also not affected by enrofloxacin after a 14-day exposure. This suggests the ability of D. rerio juveniles to adapt to enrofloxacin in a short time period. Moreover, enrofloxacin was not shown to affect collagen, cathepsin K, optic atrophy 1 and pyruvate kinase L/R mRNA expression in this study.


Assuntos
Antibacterianos/toxicidade , Enrofloxacina/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Adaptação Fisiológica , Animais , Regulação da Expressão Gênica/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Peixe-Zebra
3.
Sci Total Environ ; 651(Pt 2): 2235-2246, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30326456

RESUMO

Synthetic musk compounds are extensively used in personal care and cosmetic products around the world. Because they are not completely removed in sewage treatment plants, they eventually end up in aquatic environments. The aim of this review was to summarize published information on effects of polycyclic musks on aquatic organisms and to discuss whether the experimental design of toxicological studies involving these substances could influence the results obtained. With the exception of one study run in a flow-through system, all published toxicological studies on synthetic polycyclic musks have been conducted in semi-static or even static systems. Based upon data in the literature and our own results, we conclude that in toxicological tests with semi-static set-ups, concentrations of polycyclic musks decrease with time between bath exchanges, and, as a result, tested organisms are not being exposed to stable concentrations but rather to concentration pulses. The duration and character of these pulses are influenced mainly by aeration of experimental baths, as polycyclic musks have a tendency to volatilize from water baths. Under semi-static conditions, tested organisms may be subjected to lower concentration of the tested substance for relatively long periods. Those levels may even fall below the limits of quantification. During these periods, some level of detoxification and/or elimination (depuration) of the toxicant may reduce toxic effect of the previous exposures. Consequently, toxicity of polycyclic musk substances for aquatic organisms obtained under these conditions may be underestimated. Based upon existing data in the literature, therefore, it is very difficult to correctly estimate risk of polycyclic musks to aquatic organisms.


Assuntos
Organismos Aquáticos/efeitos dos fármacos , Produtos Domésticos/efeitos adversos , Compostos Policíclicos/efeitos adversos , Poluentes Químicos da Água/efeitos adversos
4.
Environ Sci Pollut Res Int ; 24(28): 22251-22257, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28799007

RESUMO

Synthetic musk compounds are extensively used in personal care and cosmetic products all over the world. Afterwards, they are discharged into the environment mainly because they are not completely removed in wastewater treatment plants. The aim of this study was to investigate if a passive sampler is applicable for the monitoring of tonalide, a polycyclic musk compound, in the aquatic environment and to compare the levels of tonalide in pesticide-polar organic chemical integrative sampler (POCIS) and biota. For this purpose, four sampling localities on the three biggest rivers in the Czech Republic were selected. Tonalide was determined in POCIS at all sampling sites in the concentration ranging from 9 ng/POCIS (Labe River, Hradec Králové) to 25 ng/POCIS (Morava River, Blatec). The locality with the most frequent occurrence of tonalide in biota samples was the Morava River which well corresponded with the highest tonalide concentration in POCIS among sampling sites. The highest number of positive tonalide detections among all studied biota samples was found in fish plasma. To the best of our knowledge, this is the first evidence that tonalide bioaccumulates in fish blood. Tonalide levels were below the limit of quantification in benthos samples at all sampling sites.


Assuntos
Organismos Aquáticos/química , Cosméticos/análise , Monitoramento Ambiental/métodos , Rios/química , Tetra-Hidronaftalenos/análise , Poluentes Químicos da Água/análise , Animais , Biota , República Tcheca , Monitoramento Ambiental/instrumentação , Limite de Detecção , Praguicidas/análise , Águas Residuárias/química
5.
Environ Sci Technol ; 49(5): 2625-38, 2015 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-25611781

RESUMO

Around 20 progestins (also called gestagens, progestogens, or progestagens) are used today in assisting a range of medical conditions from endometrial cancer to uterine bleeding and as an important component of oral contraception. These progestins can bind to a wide range of receptors including progestin, estrogen, androgen, glucocorticoid, and mineralocorticoid receptor, as well as sex hormone and corticosteroid binding globulins. It appears that only five of these (four synthetic and one natural) progestins have so far been studied in sewage effluent and surface waters. Analysis has reported values as either nondetects or low nanograms per liter in rivers. Seven of the progestins have been examined for their effects on aquatic vertebrates (fish and frogs). The greatest concern is associated with levonorgestrel, norethisterone, and gestodene and their ability to reduce egg production in fish at levels of 0.8-1.0 ng/L. The lack of environmental measurements, and some of the contradictions in existing values, however, hampers our ability to make a risk assessment. Only a few nanograms per liter of ethynodiol diacetate and desogestrel in water would be needed for fish to receive a human therapeutic dose for these progestins according to modeled bioconcentration factors. But for the other synthetic progestins levels would need to reach tens or hundreds of nanograms per liter to achieve a therapeutic dose. Nevertheless, the wide range of compounds, diverse receptor targets, and the effect on fish reproduction at sub-nanogram-per-liter levels should prompt further research. The ability to impair female reproduction at very low concentrations makes the progestins arguably the most important pharmaceutical group of concern after ethinylestradiol.


Assuntos
Ecotoxicologia/métodos , Ecotoxicologia/normas , Progestinas/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Peixes
6.
Biomed Res Int ; 2014: 107373, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24860807

RESUMO

The effects of Nexide (a.i. gamma-cyhalothrin 60 g L(-1)) on cumulative mortality, growth indices, and ontogenetic development of embryos and larvae of common carp (Cyprinus carpio L.) were studied. Levels of oxidative stress parameters glutathione reductase (GR), glutathione peroxidase (GPx), catalase (CAT), glutathione-S-transferase (GST), and lipid peroxidation were determined. Eggs of newly fertilised common carp were exposed to Nexide at concentrations 5, 25, 50, 100, and 250 µ g L(-1) (0.3, 1.5, 3, 6, and 15 µ g L(-1) gamma-cyhalothrin). All organisms exposed to concentrations higher than 50 µ g L(-1) died soon after hatching; at 25 µ g L(-1), 95% mortality was recorded. Larvae exposed to 5 µ g L(-1) showed significantly lower growth and retarded ontogenetic development compared to control. Histological examination of the livers of larvae from the exposed group revealed dystrophic changes. The value of detoxification enzyme GST of organisms from the exposed group was significantly higher compared to the control and the value of defensive enzyme GPx was significantly lower compared to the control. The results of our investigation confirmed that contamination of aquatic environment by pesticides containing cyhalothrin may impair growth and development of early life stages of carp and cause disbalance of defensive enzymes.


Assuntos
Carpas/embriologia , Carpas/crescimento & desenvolvimento , Desenvolvimento Embrionário/efeitos dos fármacos , Estágios do Ciclo de Vida/efeitos dos fármacos , Nitrilas/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Piretrinas/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Relação Dose-Resposta a Droga , Dose Letal Mediana , Peroxidação de Lipídeos/efeitos dos fármacos , Nitrilas/administração & dosagem , Praguicidas/toxicidade , Piretrinas/administração & dosagem , Sobrevida , Poluentes Químicos da Água/administração & dosagem
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