RESUMO
The effect of the hepatoprotective drug silymarin (Carsil) on the incorporation rate of 14C-leucine into total proteins and on the biosynthesis of UDP-N-acetylhexosamine and microsomal glycoproteins using 14C-glucose of rat liver with D-galactosamine hepatitis was studied. It was found that i.p. treatment with Carsil in a dose of 140 mg/kg applied for 4 consecutive days partly abolishes the inhibitory effect of galactosamine on protein and glycoprotein biosynthesis. The specific activity of 14C-labelled UDP-N-acetylhexosamine is higher in the liver of D-galactosamine treated rats, compared with the specific activity of the nucleotide from liver pretreated with Carsil and then injected with galactosamine. This fact supports the suggestion that Carsil probably activates the metabolic conversion of UDP-hexosamine to the acetylated metabolite-UDP-N-acetylhexosamine, which is the normal liver cell metabolite, in liver cells.
Assuntos
Flavonoides/farmacologia , Glicoproteínas/biossíntese , Hepatite Animal/metabolismo , Microssomos Hepáticos/efeitos dos fármacos , Biossíntese de Proteínas , Silimarina/farmacologia , Animais , Feminino , Galactosamina , Glucose/metabolismo , Hepatite Animal/induzido quimicamente , Leucina/metabolismo , Microssomos Hepáticos/metabolismo , Ratos , Ratos Endogâmicos , Açúcares de Uridina Difosfato/biossínteseAssuntos
Encéfalo/efeitos dos fármacos , Proteínas do Tecido Nervoso/metabolismo , Neuroglia/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Piracetam/farmacologia , Pirrolidinonas/farmacologia , Animais , Encéfalo/metabolismo , Citoplasma/efeitos dos fármacos , Citoplasma/metabolismo , Leucina/metabolismo , Neuroglia/metabolismo , Neurônios/metabolismo , Ratos , Ratos EndogâmicosRESUMO
The hepatoprotective effect of carsil (generic name silymarin) on a model of liver intoxication with D-galactosamine in rats is studied. The changes in the activity of the serum enzymes GOT, GPT, MDH, SDH, ICDH, AP. AhE and the total protein as well as the UDP-sugars content in the liver is investigated. Histochemical and electronmicroscopical investigations of the liver are carried out simultaneously. It is obvious that carsil prevents to a considerable degree the increase of the serum enzymes activity caused by a D-galactosamine injury, enhances the metabolic conversion of the UDP-hexosamine into UDP-acetylhexosamine in the liver and hastens the normalizing of the UDP-glucuronic acid content in the liver of rats. The biochemical and morphological changes under the influence of carsil and the possible biochemical mechanism of the drug action is discussed.
