RESUMO
We studied the effect of restraint stress upon the degree of lipidic peroxidation in the fronto-temporal cerebral cortex, diencephalon, thymus and gastric mucosa. We determined the malon-dialdehyde (MDA) by spectrocolorimetry, the percentage of animals with stress gastric ulcer and the surface of gastric ulcerations. The results demonstrated an important increase of the MDA correlated with frequency and intensity of the gastric ulcer. In the second stage of the experiment, we studied the influence of the ubiquinone and melatonin on the same parameters studied in the first part. In every structures these two substances had antioxidative effect. For ubiquinone, results showed significant variations in the thymus and gastric mucosa, while at the cerebral level the results were doubtful. As antioxidant and antiulcerogenic factor, melatonin was more effective than ubiquinone, because determined significant decrease, or even disappearance of the peroxidative process in the cerebral cortex and the thymus and significantly diminished both peroxidation and ulcerogenesis in the gastric mucosa.
Assuntos
Antioxidantes/metabolismo , Melatonina/metabolismo , Ubiquinona/metabolismo , Animais , Antioxidantes/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Malondialdeído/metabolismo , Melatonina/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/fisiologia , Ratos , Ratos Wistar , Restrição Física , Úlcera Gástrica/etiologia , Úlcera Gástrica/metabolismo , Estresse Psicológico/etiologia , Estresse Psicológico/metabolismo , Ubiquinona/farmacologiaRESUMO
The effect of a chronic stress upon the primary immune response on mice was evaluated by using the plaque-forming cells (PFC)-direct technique, which allowed us to record a serious suppression of the immune response in the exposed animals. In the next phase of the experiment a second group was initially treated with Tanakan (EGb 761--Ginkgo biloba extract) and then it was submitted to the same chronic stress: this procedure did not bring about the increase of the number of lymphocytes but it significantly improved the function of these cells.
Assuntos
Reações Antígeno-Anticorpo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Estresse Psicológico/tratamento farmacológico , Animais , Doença Crônica , Avaliação Pré-Clínica de Medicamentos , Ginkgo biloba , Técnica de Placa Hemolítica , Imunização/métodos , Camundongos , Extratos Vegetais/uso terapêutico , Restrição Física , Baço/efeitos dos fármacos , Baço/imunologia , Estresse Psicológico/imunologia , Fatores de TempoRESUMO
The Doppler method (pulsatile and continuous) was used for finding out and semiquantitatively evaluating the mitral insufficiency of various etiologies: inflammatory (rheumatic); prolapse of mitral valve; obstructive hypertrophic cardiomyopathy; dilatative cardiomyopathy; calcification of valvular ring. The Doppler parameters obtained after automatic processing of the image (speed transvalvular pressure gradient, flow period and acceleration) offer data on the diastolic performance of the left ventricle. Registration of the aortic flow makes possible the calculation of the cardiac flow (the diameter of the aorta is measured in echo-B), and of the aortic flow permits the noninvasive evaluation of the lung arterial pressure. The method offers a large vista in the noninvasive evaluation of the patients suffering from mitral insufficiency.
