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J Clin Virol ; 46(1): 15-9, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19505847

RESUMO

BACKGROUND: The superantigen, encoded by the envelope gene (env) of the human endogenous retrovirus (HERV)-K18, may be involved in autoimmunity. Its expression is transactivated in B cells during infection with Epstein-Barr virus and in peripheral blood mononuclear cells (PBMCs) that are treated with IFN-alpha. OBJECTIVES: We investigated whether HHV-6B infection was able to induce the expression of HERV-K18 env. STUDY DESIGN: The expression of HERV-K18 env gene was measured by real-time quantitative PCR in HHV-6B-infected PBMC. RESULTS: Infection of PBMCs with HHV-6B resulted in a rapid and dose-dependent induction of HERV-K18 env gene expression, predominantly in monocytes. Induction was dependent on the interaction of glycoprotein H with CD46, but did not require viral transcription or DNA synthesis. Cycloheximide inhibited both the induction and basal expression of HERV-K18 env, indicating that de novo synthesis of proteins was necessary. CONCLUSIONS: HHV-6B induced transcriptional activation of the endogenous superantigen HERV-K18 independently of virus replication, which may have consequences for the development of autoimmunity.


Assuntos
Retrovirus Endógenos/fisiologia , Perfilação da Expressão Gênica , Herpesvirus Humano 6/fisiologia , Proteínas de Membrana/biossíntese , Superantígenos/biossíntese , Células Cultivadas , Humanos , Leucócitos Mononucleares/virologia , Proteínas de Membrana/genética , Superantígenos/genética
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