RESUMO
The localization of an ouabain-sensitive potassium-dependent p-nitrophenylphosphatase part of the Na+,K(+)-ATPase complex in the white rat's brain has been studied at the ultrastructural level. The physiological pH of incubation medium highly increases the specificity of ultracytochemical enzyme demonstration. The main characteristics of the enzymatic p-NPP hydrolysis used for this methodological technique are discussed.
Assuntos
4-Nitrofenilfosfatase/metabolismo , Córtex Cerebral/enzimologia , Ouabaína/farmacologia , Potássio/metabolismo , ATPase Trocadora de Sódio-Potássio/metabolismo , 4-Nitrofenilfosfatase/efeitos dos fármacos , Animais , Transporte Biológico Ativo/efeitos dos fármacos , Córtex Cerebral/efeitos dos fármacos , Córtex Cerebral/ultraestrutura , Histocitoquímica , Concentração de Íons de Hidrogênio , Masculino , Microscopia Eletrônica , Fibras Nervosas Mielinizadas/efeitos dos fármacos , Fibras Nervosas Mielinizadas/enzimologia , Fibras Nervosas Mielinizadas/ultraestrutura , Ratos , ATPase Trocadora de Sódio-Potássio/efeitos dos fármacosRESUMO
The authors discuss the results of electron-microscopic study of the synaptic contacts in the brain of rabbits during 4 months after experimental brain concussion. A complex of ultrastructural changes of the cerebral synaptic apparatus with a characteristic dynamics of development in time and space is described, and its peculiarities and features distinguishing it from other types of pathology as well as its role in the pathogenesis of craniocerebral injury are discussed.
Assuntos
Concussão Encefálica/patologia , Encéfalo/ultraestrutura , Sinapses/ultraestrutura , Animais , Hipotálamo Posterior/ultraestrutura , Masculino , Microscopia Eletrônica , Córtex Motor/ultraestrutura , Coelhos , Córtex Somatossensorial/ultraestrutura , Fatores de TempoRESUMO
Electron microscopy of two hundred brain tumours of various origin allowed the authors to elaborate the main principles of electron microscopic diagnosis. A comprehensive approach to determining the comparative diagnostic value of the morphological signs of the tumour and the initial normal tissue and the high resolving capacity of the electron microscope provide for trustworthy identification of tissue origin of the new growth that is examined. The electron microscopic method is particularly important when diagnostic difficulties arise on the light-optic level of the morphological examination.
Assuntos
Neoplasias Encefálicas/ultraestrutura , Astrócitos/ultraestrutura , Astrocitoma/ultraestrutura , Neoplasias Cerebelares/ultraestrutura , Neoplasias do Ventrículo Cerebral/ultraestrutura , Plexo Corióideo , Ependimoma/ultraestrutura , Hemangioendotelioma/ultraestrutura , Hemangiossarcoma/ultraestrutura , Humanos , Microscopia Eletrônica , Papiloma/ultraestruturaRESUMO
Electron-microscopy of tumours, diagnosed by light optics as ependymoastrocytomas, helped to ascertain the common features in the ultrastructural organization of their tumorous tissue: a complex of organ-specific signs of ependymomas is constantly manifested in all the scrutinized parts of the tumorous nodes. This makes it possible to regard ependymoastrocytomas as a variant of ependymomas and not tumours of a mixed origin.