The Impact of a Self-Avatar, Hand Collocation, and Hand Proximity on Embodiment and Stroop Interference.

Understanding the effects of hand proximity to objects and tasks is critical for hand-held and near-hand objects. Even though self-avatars have been shown to be beneficial for various tasks in virtual environments, little research has investigated the effect of avatar hand proximity on working memory. This paper presents a between-participants user study investigating the effects of self-avatars and physical hand proximity on a common working memory task, the Stroop interference task. Results show that participants felt embodied when a self-avatar was in the scene, and that the subjective level of embodiment decreased when a participant's hands were not collocated with the avatar's hands. Furthermore, a participant's physical hand placement was significantly related to Stroop interference: proximal hands produced a significant increase in accuracy compared to non-proximal hands. Surprisingly, Stroop interference was not mediated by the existence of a self-avatar or level of embodiment.

Attempted use of PACE for riboswitch discovery generates three new translational theophylline riboswitch side products.

OBJECTIVE: The purpose of this project was to use an in vivo method to discover riboswitches that are activated by new ligands. We employed phage-assisted continuous evolution (PACE) to evolve new riboswitches in vivo. We started with one translational riboswitch and one transcriptional riboswitch, both of which were activated by theophylline. We used xanthine as the new target ligand during positive selection followed by negative selection using theophylline. The goal was to generate very large M13 phage populations that contained unknown mutations, some of which would result in new aptamer specificity. We discovered side products of three new theophylline translational riboswitches with different levels of protein production. RESULTS: We used next generation sequencing to identify M13 phage that carried riboswitch mutations. We cloned and characterized the most abundant riboswitch mutants and discovered three variants that produce different levels of translational output while retaining their theophylline specificity. Although we were unable to demonstrate evolution of new riboswitch ligand specificity using PACE, we recommend careful design of recombinant M13 phage to avoid evolution of "cheaters" that short circuit the intended selection pressure.